过氧化尿素与微生物联合修复石油污染土壤

doi:10.16085/j.issn.1000-6613.2021-2444

化工进展 ›› 2022, Vol. 41 ›› Issue (9): 5085-5093.DOI: 10.16085/j.issn.1000-6613.2021-2444

过氧化尿素与微生物联合修复石油污染土壤

郑瑾¹^,²(), 韩瑞瑞¹^,²^,³(), 李丹丹¹^,², 王馨妤¹^,²^,⁴, 高春阳¹^,², 杜显元¹^,²(), 张晓飞¹^,², 邹德勋³

^1.石油石化污染物控制与处理国家重点实验室，北京 102206
^2.中国石油集团安全环保技术研究院有限公司，北京 102206
^3.北京化工大学化学工程学院，北京 100029
^4.中国石油大学（北京）化学工程与环境学院，北京 102249

收稿日期:2021-11-29 修回日期:2022-03-16 出版日期:2022-09-25 发布日期:2022-09-27
通讯作者: 杜显元
作者简介:郑瑾（1985—），女，博士，高级工程师，研究方向为土壤修复、环境生物技术。E-mail：zhengjin2810@163.com
韩瑞瑞（1995—），男，硕士研究生，研究方向为土壤修复。E-mail：hanrr2020@163.com。
基金资助:
国家重点研发计划(2019YFC1806004);基础科学和战略储备技术研究基金(2020D-5008)

Joint remediation of petroleum contaminated soil by urea peroxide with microorganism

ZHENG Jin¹^,²(), HAN Ruirui¹^,²^,³(), LI Dandan¹^,², WANG Xinyu¹^,²^,⁴, GAO Chunyang¹^,², DU Xianyuan¹^,²(), ZHANG XiaoFei¹^,², ZOU Dexun³

^1.State Key Lab of Petroleum Pollution Control, Beijing 102206, China
^2.CNPC Research Institute of Safety & Environmental Technology, Beijing 102206, China
^3.Beijing University of Chemical Technology, School of Chemical Engineering, Beijing 100029, China
^4.China University of Petroleum-Beijing, College of Chemical Engineering and Environment, Beijing 102249, China

Received:2021-11-29 Revised:2022-03-16 Online:2022-09-25 Published:2022-09-27
Contact: DU Xianyuan

摘要/Abstract

摘要：

为揭示化学氧化法与微生物法联合修复技术在石油污染土壤修复中应用可行性，本研究采用正交实验设计和联合修复实验，以过氧化尿素（UHP）为氧化剂，Fe²⁺为激活剂，研究了UHP去除石油烃的条件及其与微生物联合修复土壤石油污染物的效果。结果表明，利用UHP氧化土壤中石油烃的最佳条件为：UHP添加质量分数4%、UHP∶Fe²⁺（摩尔比）=30∶1及水∶土=3∶2。过氧化尿素氧化法与微生物法联合作用下，石油烃降解率比单一微生物修复法和过氧化尿素氧化修复法分别提高了35.45%~47.26%和3.35%~15.16%。过氧化尿素与微生物的修复顺序优化结果显示，过氧化尿素预氧化联合微生物修复比先进行微生物修复再进行过氧化尿素氧化对土壤中石油烃的降解率提高了11.81%。研究结果证明过氧化尿素联合微生物修复对降解土壤中石油烃污染的可行性。

关键词: 过氧化尿素, 土壤修复, 正交实验, 活化（作用）, 石油污染

Abstract:

In order to certificate the feasibility of joint remediation of chemical oxidation method with microorganism method for petroleum-contaminated soil. Using urea peroxide as the oxidant and Fe²⁺ as the activator, joint repair experiments and the orthogonal experiment design were used to investigate the conditions for urea peroxide (UHP) to remove petroleum pollutants in soil and the effect of UHP combined with microorganism on the remediation of petroleum contaminated soil. The optimal conditions for the chemical oxidation experiment were as followings: 4% mass fraction of UHP, UHP∶Fe²⁺ (molar ratio) = 30∶1 and water∶soil = 3∶2. Compared to the single microbial remediation method and single urea peroxide oxidation remediation method, the degradation rate of petroleum hydrocarbons with the urea peroxide combined with microbial methods for remediation of petroleum contaminated soils was increased by 35.45%—47.26% and 3.35%—15.16%, respectively. The sequence of urea peroxide and microorganism remediation was optimized. Compared to microbial remediation followed by urea peroxide remediation, the urea peroxide remediation followed by microbial remediation increased the degradation rate of petroleum hydrocarbons by 11.81% . This study shows that urea peroxide combined with microbial remediation is a good way to degrade petroleum in the soil.

Key words: urea peroxide, soil remediation, orthogonal experimental, activation, petroleum contamination

中图分类号:

郑瑾, 韩瑞瑞, 李丹丹, 王馨妤, 高春阳, 杜显元, 张晓飞, 邹德勋. 过氧化尿素与微生物联合修复石油污染土壤[J]. 化工进展, 2022, 41(9): 5085-5093.

ZHENG Jin, HAN Ruirui, LI Dandan, WANG Xinyu, GAO Chunyang, DU Xianyuan, ZHANG XiaoFei, ZOU Dexun. Joint remediation of petroleum contaminated soil by urea peroxide with microorganism[J]. Chemical Industry and Engineering Progress, 2022, 41(9): 5085-5093.

图/表 11

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指标名称	值
石油烃/mg·kg^-1	44191
含水率/%	1.54
pH	8.17
有机碳/g·kg^-1	71.19
（总氮/总磷）/g·kg^-1	1.19/0.57
氨氮/mg·kg^-1	2.72
饱和烃/%	56.12
芳香烃/%	23.60
胶质/%	20.28

指标名称	值
石油烃/mg·kg^-1	44191
含水率/%	1.54
pH	8.17
有机碳/g·kg^-1	71.19
（总氮/总磷）/g·kg^-1	1.19/0.57
氨氮/mg·kg^-1	2.72
饱和烃/%	56.12
芳香烃/%	23.60
胶质/%	20.28

水平	UHP质量分数（A）/%	UHP∶Fe²⁺ （E）	水∶土（C）
1	1	10∶1	1∶2
2	2	30∶1	1∶1
3	4	50∶1	3∶2

水平	UHP质量分数（A）/%	UHP∶Fe²⁺ （E）	水∶土（C）
1	1	10∶1	1∶2
2	2	30∶1	1∶1
3	4	50∶1	3∶2

实验序号	水平组合	实验条件
实验序号	水平组合	UHP质量分数（A）/%	UHP∶Fe ²⁺（E）	水∶土（C）
1	A₁E₁C₁	1	10∶1	1∶2
2	A₁E₂C₂	1	30∶1	1∶1
3	A₁E₃C₃	1	50∶1	3∶2
4	A₂E₁C₂	2	10∶1	1∶1
5	A₂E₂C₃	2	30∶1	3∶2
6	A₂E₃C₁	2	50∶1	1∶2
7	A₃E₁C₃	4	10∶1	3∶2
8	A₃E₂C₁	4	30∶1	1∶2
9	A₃E₃C₂	4	50∶1	1∶1

过氧化尿素与微生物联合修复石油污染土壤

Joint remediation of petroleum contaminated soil by urea peroxide with microorganism

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图/表 11

参考文献 38

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Metrics

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处理组	实验方法
CK	菌剂及氧化剂无添加
B	10% 的菌剂
UF	UHP（4%，质量分数）、UHP∶Fe ²⁺ （摩尔比）=30∶1
UF+B	UF为UHP（4%，质量分数）、UHP∶Fe ²⁺ （摩尔比）= 30∶1；B为10%的菌剂
B+UF	B为10%的菌剂；UF为UHP（4%，质量分数）、 UHP∶Fe ²⁺ （摩尔比）=30∶1

实验序号	实验条件			石油降解率/%
实验序号	UHP质量分数（A）	UHP∶Fe ²⁺ （E）	水∶土（C）	石油降解率/%
1	1%	10∶1	1∶2	27.85
2	1%	30∶1	1∶1	31.66
3	1%	50∶1	3∶2	29.39
4	2%	10∶1	1∶1	33.58
5	2%	30∶1	3∶2	34.34
6	2%	50∶1	1∶2	29.39
7	4%	10∶1	3∶2	38.53
8	4%	30∶1	1∶2	40.81
9	4%	50∶1	1∶1	34.34
k₁	87.98	99.50	97.39
k₂	97.06	106.25	99.21
k₃	112.90	92.18	101.35
k₁	29.33	33.17	32.46
k₂	32.35	35.42	33.07
k₃	37.63	30.73	33.78
R	8.31	4.69	1.32

组别	Ace	Chao	Shannon	Simpson	Coverage
CK	1028	1038	4.95	0.018	0.997
UF	903	876	3.85	0.073	0.993
B	839	826	3.48	0.110	0.996
UF+B	825	844	4.11	0.041	0.997
B+UF	961	828	3.72	0.051	0.996

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