大肠杆菌调控因子工程及其菌株耐受性研究进展

doi:10.16085/j.issn.1000-6613.2017-1696

化工进展 ›› 2018, Vol. 37 ›› Issue (07): 2780-2789.DOI: 10.16085/j.issn.1000-6613.2017-1696

大肠杆菌调控因子工程及其菌株耐受性研究进展

宋鑫^1,2, 张双虹^2,3,4, 陈涛^2,3,4, 刘训理¹, 王智文^2,3,4

1 山东农业大学林学院, 山东泰安 271018;
2 天津大学化工学院, 天津 300350;
3 天津大学系统生物工程教育部重点实验室, 天津 300350;
4 天津化学化工协同创新中心, 天津 300350

收稿日期:2017-08-11 修回日期:2017-09-03 出版日期:2018-07-05 发布日期:2018-07-05
通讯作者: 刘训理,教授,研究方向为微生物资源与应用;王智文,副教授,研究方向为微生物代谢工程与合成生物学。
作者简介:宋鑫(1991-),女,硕士研究生。
基金资助:
国家自然科学基金（21776209，21576200，21621004，21390201）、天津市自然科学基金（15JCQNJC06000）、国家农业公益性行业专项（201503112-10）和山东省“双一流”建设项目。

Research progress of regulator engineering and stress tolerances with Escherichia coli

SONG Xin^1,2, ZHANG Shuanghong^2,3,4, CHEN Tao^2,3,4, LIU Xunli¹, WANG Zhiwen^2,3,4

1 College of Forestry, Shandong Agricultural University, Taian 271018, Shandong, China;
2 School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, China;
3 Key Laboratory of Systems Bioengineering(Ministry of Education), Tianjin University, Tianjin 300350, China;
4 SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering(Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, China

Received:2017-08-11 Revised:2017-09-03 Online:2018-07-05 Published:2018-07-05

摘要/Abstract

摘要： 大肠杆菌具有金字塔式的基因表达等级调控网络，调控因子的自动调控、共调控和交叉调控，构成了复杂而又精细的转录调控网络。微生物通过扰动和优化这个高效的调控网络快速地响应环境变化，而适应新的耐受条件。微生物的耐受性是由多基因控制的复杂表型，通过大肠杆菌调控因子工程，可以大尺度重构调控网络，显著改进菌株耐受性，成为了近几年的研究热点。本文总结了大肠杆菌调控因子及其工程方法，综述了通过大肠杆菌调控因子工程重构代谢网络来提高菌株耐受性的最新研究进展，展望了通过大肠杆菌调控因子工程提高菌株鲁棒性的发展方向。

关键词: 大肠杆菌, 调控因子, 鲁棒性, 耐受性, 调控网络

Abstract: Escherichia coli (E.coli) has intricate pyramid-shaped hierarchical regulatory networks regulated by regulators. The auto-regulation, co-regulation, and cross-talk regulation among regulators further complicate this regulatory network. Microbes can quickly respond to transitory environmental changes and optimize their metabolism to adapt to new conditions by modifying this intricate and efficient transcriptional regulatory network. In recent years, more and more researchers pay attention to engineer regulators of E. coli to improve its stress tolerance. This paper summarized the regulators of E. coli and their engineering methods, and reviewed the current state-of-the-art stress tolerance improvement with E. coli induced by rewriting its metabolic network through regulator engineering. While current regulator engineering mainly focuses on limited global regulators, novel engineering tools are needed to be developed and more regulators should be mutated for their corresponding genotype-phenotype analysis for further robust strain construction.

Key words: E. coli, regulator, robust, stress tolerance, regulatory network

中图分类号:

Q756
Q815

宋鑫, 张双虹, 陈涛, 刘训理, 王智文. 大肠杆菌调控因子工程及其菌株耐受性研究进展[J]. 化工进展, 2018, 37(07): 2780-2789.

SONG Xin, ZHANG Shuanghong, CHEN Tao, LIU Xunli, WANG Zhiwen. Research progress of regulator engineering and stress tolerances with Escherichia coli[J]. Chemical Industry and Engineering Progress, 2018, 37(07): 2780-2789.

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大肠杆菌调控因子工程及其菌株耐受性研究进展

Research progress of regulator engineering and stress tolerances with Escherichia coli

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