化工进展 ›› 2022, Vol. 41 ›› Issue (12): 6531-6539.DOI: 10.16085/j.issn.1000-6613.2022-0459
收稿日期:
2022-03-23
修回日期:
2022-04-12
出版日期:
2022-12-20
发布日期:
2022-12-29
通讯作者:
诸葛斌
作者简介:
郭超(1996—),男,硕士研究生,研究方向为微生物代谢工程。E-mail:13008078449@163.com。
GUO Chao(), FENG Ao, LU Xinyao, ZONG Hong, ZHUGE Bin()
Received:
2022-03-23
Revised:
2022-04-12
Online:
2022-12-20
Published:
2022-12-29
Contact:
ZHUGE Bin
摘要:
1,2,4-丁三醇(1,2,4-butantriol,BT)属于非天然化学品,是军工材料1,2,4-丁三醇三硝酸酯的前体。在重组大肠杆菌中,木糖经脱氢、脱水、脱羧和还原合成BT。但途径各反应不平衡使得中间代谢物积累限制菌体生长和产物合成。本研究首先通过CRISPR/Cas9敲除基因yjhG和yqhD构建无本底表达宿主菌,随后利用不同启动子组合调节BT合成途径中基因xdh、yjhG和yqhD的表达。结果发现,以P inv 表达醇脱氢酶基因yqhD使BT产量达到14.4g/L;以P tac 表达脱氢酶基因xdh和P rrnH P1表达脱水酶基因yjhG的组合方式,BT产量达到15.6g/L,比对照菌株KXW3009分别增加5.9%和14.7%。本研究通过对中间代谢物木糖酸合成和代谢的组合优化,促进了BT的合成,为后续研究提供了借鉴。
中图分类号:
郭超, 冯奥, 陆信曜, 宗红, 诸葛斌. 重组大肠杆菌1,2,4-丁三醇合成途径的平衡优化[J]. 化工进展, 2022, 41(12): 6531-6539.
GUO Chao, FENG Ao, LU Xinyao, ZONG Hong, ZHUGE Bin. Balanced optimization of the 1,2,4-butanetriol synthesis pathway in recombinant Escherichia coli[J]. Chemical Industry and Engineering Progress, 2022, 41(12): 6531-6539.
菌株和质粒 | 相关特性 | 来源 |
---|---|---|
E.coli W3009(W3009) | E.coli W3110 ΔxylABΔyagEΔyjhHΔptsHIΔmgsA | 实验室保存 |
E.coli W3010(W3010) | E.coli W3009 ΔyjhGΔyqhD | 本研究 |
E.coli KXW3009(KXW3009) | E.coli W3009,pEtac-kivD-tac-xdh | 实验室保存 |
E.coli KXW3010(KXW3010) | E.coli W3010,pEtac-kivD-tac-xdh | 本研究 |
E.coli KXW3010P0(P0) | E.coli KXW3010,pRSFDuet-P yjhG -yjhG | 本研究 |
E.coli KXW3010P1(P1) | E.coli KXW3010,pRSFDuet-P yjhG -yjhG-P yqhD-yqhD | 本研究 |
E.coli KXW3010P2(P2) | E.coli KXW3010,pRSFDuet-P yjhG -yjhG-tac-yqhD | 本研究 |
E.coli KXW3010P3(P3) | E.coli KXW3010,pRSFDuet-P yjhG -yjhG-inv-yqhD | 本研究 |
E.coli KXW3010P4(P4) | E.coli KXW3010,pRSFDuet-P yjhG -yjhG-dnaKJ-yqhD | 本研究 |
E.coli KXW3010P5(P5) | E.coli KXW3010,pRSFDuet-P yjhG -yjhG-grpE-yqhD | 本研究 |
E.coli KXW3010P3-12(P12) | E.coli W3010,pRSF-P yjhG -yjhG-inv-yqhD,pEtac-kivD-rpsA P1-xdh | 本研究 |
E.coli KXW3010P3-13(P13) | E.coli W3010,pRSF-P yjhG -yjhG-inv-yqhD,pEtac-kivD-rpsT P1-xdh | 本研究 |
E.coli KXW3010P3-14(P14) | E.coli W3010,pRSF-P yjhG -yjhG-inv-yqhD,pEtac-kivD-inv-xdh | 本研究 |
E.coli KXW3010P3-15(P15) | E.coli W3010,pRSF-P yjhG -yjhG-inv-yqhD,pEtac-kivD-dnaKJ-xdh | 本研究 |
E.coli KXW3010P3-16(P16) | E.coli W3010,pRSF-P yjhG -yjhG-inv-yqhD,pEtac-kivD-grpE-xdh | 本研究 |
E.coli KXW3010P3-21(P21) | E.coli W3010,pRSFtac-yjhG-inv-yqhD,pEtac-kivD-tac-xdh | 本研究 |
E.coli KXW3010P3-22(P22) | E.coli W3010,pRSFtac-yjhG-inv-yqhD,pEtac-kivD-rpsA P1-xdh | 本研究 |
E.coli KXW3010P3-23(P23) | E.coli W3010,pRSFtac-yjhG-inv-yqhD,pEtac-kivD-rpsT P1-xdh | 本研究 |
E.coli KXW3010P3-24(P24) | E.coli W3010,pRSFtac-yjhG-inv-yqhD,pEtac-kivD-inv-xdh | 本研究 |
E.coli KXW3010P3-25(P25) | E.coli W3010,pRSFtac-yjhG-inv-yqhD,pEtac-kivD-dnaKJ-xdh | 本研究 |
E.coli KXW3010P3-26(P26) | E.coli W3010,pRSFtac-yjhG-inv-yqhD,pEtac-kivD-grpE-xdh | 本研究 |
E.coli KXW3010P3-31(P31) | E.coli W3010,pRSFrrnC P1-yjhG-inv-yqhD,pEtac-kivD-tac-xdh | 本研究 |
E.coli KXW3010P3-32(P32) | E.coli W3010,pRSFrrnC P1-yjhG-inv-yqhD,pEtac-kivD-rpsA P1-xdh | 本研究 |
E.coli KXW3010P3-33(P33) | E.coli W3010,pRSFrrnC P1-yjhG-inv-yqhD,pEtac-kivD-rpsT P1-xdh | 本研究 |
E.coli KXW3010P3-34(P34) | E.coli W3010,pRSFrrnC P1-yjhG-inv-yqhD,pEtac-kivD-inv-xdh | 本研究 |
E.coli KXW3010P3-35(P35) | E.coli W3010,pRSFrrnC P1-yjhG-inv-yqhD,pEtac-kivD-dnaKJ-xdh | 本研究 |
E.coli KXW3010P3-36(P36) | E.coli W3010,pRSFrrnC P1-yjhG-inv-yqhD,pEtac-kivD-grpE-xdh | 本研究 |
E.coli KXW3010P3-41(P41) | E.coli W3010,pRSFrrnH P1-yjhG-inv-yqhD,pEtac-kivD-tac-xdh | 本研究 |
E.coli KXW3010P3-42(P42) | E.coli W3010,pRSFrrnH P1-yjhG-inv-yqhD,pEtac-kivD-rpsA P1-xdh | 本研究 |
E.coli KXW3010P3-43(P43) | E.coli W3010,pRSFrrnH P1-yjhG-inv-yqhD,pEtac-kivD-rpsT P1-xdh | 本研究 |
E.coli KXW3010P3-44(P44) | E.coli W3010,pRSFrrnH P1-yjhG-inv-yqhD,pEtac-kivD-inv-xdh | 本研究 |
E.coli KXW3010P3-45(P45) | E.coli W3010,pRSFrrnH P1-yjhG-inv-yqhD,pEtac-kivD-dnaKJ-xdh | 本研究 |
E.coli KXW3010P3-46(P46) | E.coli W3010,pRSFrrnH P1-yjhG-inv-yqhD,pEtac-kivD-grpE-xdh | 本研究 |
E.coli KXW3010P3-51(P51) | E.coli W3010,pRSFdnaKJrpsT -yjhG-inv-yqhD,pEtac-kivD-tac-xdh | 本研究 |
E.coli KXW3010P3-52(P52) | E.coli W3010,pRSFdnaKJrpsT -yjhG-inv-yqhD,pEtac-kivD-rpsA P1-xdh | 本研究 |
E.coli KXW3010P3-53(P53) | E.coli W3010,pRSFdnaKJrpsT -yjhG-inv-yqhD,pEtac-kivD-rpsT P1-xdh | 本研究 |
E.coli KXW3010P3-54(P54) | E.coli W3010,pRSFdnaKJrpsT -yjhG-inv-yqhD,pEtac-kivD-inv-xdh | 本研究 |
E.coli KXW3010P3-55(P55) | E.coli W3010,pRSFdnaKJrpsT -yjhG-inv-yqhD,pEtac-kivD-dnaKJ-xdh | 本研究 |
E.coli KXW3010P3-56(P56) | E.coli W3010,pRSFdnaKJrpsT -yjhG-inv-yqhD,pEtac-kivD-grpE-xdh | 本研究 |
pCas9 | P-cas9, Kanr | 实验室保存 |
pTargetF-PMB | pTarget added sgRNA, Sper | 实验室保存 |
pEtac-kivD-tac-xdh | Kanr, tac启动子 | 实验室保存 |
表1 本研究使用的菌株和质粒
菌株和质粒 | 相关特性 | 来源 |
---|---|---|
E.coli W3009(W3009) | E.coli W3110 ΔxylABΔyagEΔyjhHΔptsHIΔmgsA | 实验室保存 |
E.coli W3010(W3010) | E.coli W3009 ΔyjhGΔyqhD | 本研究 |
E.coli KXW3009(KXW3009) | E.coli W3009,pEtac-kivD-tac-xdh | 实验室保存 |
E.coli KXW3010(KXW3010) | E.coli W3010,pEtac-kivD-tac-xdh | 本研究 |
E.coli KXW3010P0(P0) | E.coli KXW3010,pRSFDuet-P yjhG -yjhG | 本研究 |
E.coli KXW3010P1(P1) | E.coli KXW3010,pRSFDuet-P yjhG -yjhG-P yqhD-yqhD | 本研究 |
E.coli KXW3010P2(P2) | E.coli KXW3010,pRSFDuet-P yjhG -yjhG-tac-yqhD | 本研究 |
E.coli KXW3010P3(P3) | E.coli KXW3010,pRSFDuet-P yjhG -yjhG-inv-yqhD | 本研究 |
E.coli KXW3010P4(P4) | E.coli KXW3010,pRSFDuet-P yjhG -yjhG-dnaKJ-yqhD | 本研究 |
E.coli KXW3010P5(P5) | E.coli KXW3010,pRSFDuet-P yjhG -yjhG-grpE-yqhD | 本研究 |
E.coli KXW3010P3-12(P12) | E.coli W3010,pRSF-P yjhG -yjhG-inv-yqhD,pEtac-kivD-rpsA P1-xdh | 本研究 |
E.coli KXW3010P3-13(P13) | E.coli W3010,pRSF-P yjhG -yjhG-inv-yqhD,pEtac-kivD-rpsT P1-xdh | 本研究 |
E.coli KXW3010P3-14(P14) | E.coli W3010,pRSF-P yjhG -yjhG-inv-yqhD,pEtac-kivD-inv-xdh | 本研究 |
E.coli KXW3010P3-15(P15) | E.coli W3010,pRSF-P yjhG -yjhG-inv-yqhD,pEtac-kivD-dnaKJ-xdh | 本研究 |
E.coli KXW3010P3-16(P16) | E.coli W3010,pRSF-P yjhG -yjhG-inv-yqhD,pEtac-kivD-grpE-xdh | 本研究 |
E.coli KXW3010P3-21(P21) | E.coli W3010,pRSFtac-yjhG-inv-yqhD,pEtac-kivD-tac-xdh | 本研究 |
E.coli KXW3010P3-22(P22) | E.coli W3010,pRSFtac-yjhG-inv-yqhD,pEtac-kivD-rpsA P1-xdh | 本研究 |
E.coli KXW3010P3-23(P23) | E.coli W3010,pRSFtac-yjhG-inv-yqhD,pEtac-kivD-rpsT P1-xdh | 本研究 |
E.coli KXW3010P3-24(P24) | E.coli W3010,pRSFtac-yjhG-inv-yqhD,pEtac-kivD-inv-xdh | 本研究 |
E.coli KXW3010P3-25(P25) | E.coli W3010,pRSFtac-yjhG-inv-yqhD,pEtac-kivD-dnaKJ-xdh | 本研究 |
E.coli KXW3010P3-26(P26) | E.coli W3010,pRSFtac-yjhG-inv-yqhD,pEtac-kivD-grpE-xdh | 本研究 |
E.coli KXW3010P3-31(P31) | E.coli W3010,pRSFrrnC P1-yjhG-inv-yqhD,pEtac-kivD-tac-xdh | 本研究 |
E.coli KXW3010P3-32(P32) | E.coli W3010,pRSFrrnC P1-yjhG-inv-yqhD,pEtac-kivD-rpsA P1-xdh | 本研究 |
E.coli KXW3010P3-33(P33) | E.coli W3010,pRSFrrnC P1-yjhG-inv-yqhD,pEtac-kivD-rpsT P1-xdh | 本研究 |
E.coli KXW3010P3-34(P34) | E.coli W3010,pRSFrrnC P1-yjhG-inv-yqhD,pEtac-kivD-inv-xdh | 本研究 |
E.coli KXW3010P3-35(P35) | E.coli W3010,pRSFrrnC P1-yjhG-inv-yqhD,pEtac-kivD-dnaKJ-xdh | 本研究 |
E.coli KXW3010P3-36(P36) | E.coli W3010,pRSFrrnC P1-yjhG-inv-yqhD,pEtac-kivD-grpE-xdh | 本研究 |
E.coli KXW3010P3-41(P41) | E.coli W3010,pRSFrrnH P1-yjhG-inv-yqhD,pEtac-kivD-tac-xdh | 本研究 |
E.coli KXW3010P3-42(P42) | E.coli W3010,pRSFrrnH P1-yjhG-inv-yqhD,pEtac-kivD-rpsA P1-xdh | 本研究 |
E.coli KXW3010P3-43(P43) | E.coli W3010,pRSFrrnH P1-yjhG-inv-yqhD,pEtac-kivD-rpsT P1-xdh | 本研究 |
E.coli KXW3010P3-44(P44) | E.coli W3010,pRSFrrnH P1-yjhG-inv-yqhD,pEtac-kivD-inv-xdh | 本研究 |
E.coli KXW3010P3-45(P45) | E.coli W3010,pRSFrrnH P1-yjhG-inv-yqhD,pEtac-kivD-dnaKJ-xdh | 本研究 |
E.coli KXW3010P3-46(P46) | E.coli W3010,pRSFrrnH P1-yjhG-inv-yqhD,pEtac-kivD-grpE-xdh | 本研究 |
E.coli KXW3010P3-51(P51) | E.coli W3010,pRSFdnaKJrpsT -yjhG-inv-yqhD,pEtac-kivD-tac-xdh | 本研究 |
E.coli KXW3010P3-52(P52) | E.coli W3010,pRSFdnaKJrpsT -yjhG-inv-yqhD,pEtac-kivD-rpsA P1-xdh | 本研究 |
E.coli KXW3010P3-53(P53) | E.coli W3010,pRSFdnaKJrpsT -yjhG-inv-yqhD,pEtac-kivD-rpsT P1-xdh | 本研究 |
E.coli KXW3010P3-54(P54) | E.coli W3010,pRSFdnaKJrpsT -yjhG-inv-yqhD,pEtac-kivD-inv-xdh | 本研究 |
E.coli KXW3010P3-55(P55) | E.coli W3010,pRSFdnaKJrpsT -yjhG-inv-yqhD,pEtac-kivD-dnaKJ-xdh | 本研究 |
E.coli KXW3010P3-56(P56) | E.coli W3010,pRSFdnaKJrpsT -yjhG-inv-yqhD,pEtac-kivD-grpE-xdh | 本研究 |
pCas9 | P-cas9, Kanr | 实验室保存 |
pTargetF-PMB | pTarget added sgRNA, Sper | 实验室保存 |
pEtac-kivD-tac-xdh | Kanr, tac启动子 | 实验室保存 |
引物名称 | 引物序列(5′→3′) | 质粒构建或功能 |
---|---|---|
Sg-yjhG-F | tcaaaaaggcatcaccacccGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTC | pTargetF-PMB(yjhG) |
Sg-yjhG-R | gggtggtgatgcctttttgaACTAGTATTATACCTAGGACTGAGCTAGCTGTCAAG | |
Sg-yqhD-F | gtgatctcccgtaaaaccacGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTC | pTargetF-PMB(yqhD) |
Sg-yqhD-R | gtggttttacgggagatcacACTAGTATTATACCTAGGACTGAGCTAGCTGTCAAG | |
dornor-yjhG-F | TGGCGGGAGATCGCCAGAGCTTCCGCGCGAGCTGCGCTGAACCTATATTGCGCC | 融合片段dornor(yjhG) |
dornor-yjhG-R | TCAATGGCGGTGGATTTGATCACCGAACCTTCTGGCGCAATATAGGTTCAGCGCAG | |
dornor-yqhD-F | TGTGTGCTGACGCTGCCAGCAACCGGTTCAGAATCCAACGCAGGCATCACTGAAGG | 融合片段dornor(yqhD) |
dornor-yqhD-R | GGCAATCGCGGCGTCAATACGCTCATCATCGGAACCTTCAGTGATGCCTGCGTTGG | |
yjhG-F | aatttcacacaggaaacaGTGATCCTGTACAACTTTCCGG | pRSFDuet-P yjhG -yjhG |
yjhG-R | cctgcaggcgcgccgGGTACCAGAAGAATTAAACCGACTTCG | |
yqhD-F | cggcgcgcctgcaggGTCCATCAACAGTTCCTGTAACTGC | pRSFDuet-P yjhG -yjhG-P yqhD-yqhD |
yqhD-R | tcgacttaagcattatgcAGTGGTGATGAACAGTTCTTCTCTG | |
tac-yqhD-F | cggcgcgcctgcaggGAGGGAGCTTATCGACTGCACGG | pRSFDuet-P yjhG -yjhG-tac-yqhD |
tac-yqhD-R | TGTTTCCTGTGTGAAATTGTTATCCGCTCAC | |
yqhD-tac-F | aatttcacacaggaaacaGAAGTAATGAACAACTTTAATCTGCACACCCC | |
yqhD-tac-R | tcgacttaagcattatgcGAGGCGTAAAAAGCTTAGCGGGC | |
inv-yqhD-F | cggcgcgcctgcaggAACATTGAGGCAACGACAAGGTCGATTCTGCTATCCTTG AGGAGGCTCCTCGTAATGAACAACTTTAATCTGCACACCCC | pRSF-P yjhG -yjhG-inv-yqhD |
inv-yqhD-R | tcgacttaagcattatgcGAGGCGTAAAAAGCTTAGCGGGC | |
dnaKJ-yqhD-F | cggcgcgcctgcaggCAGGAAACATTTTTTGATGAATGCCTTGGC | pRSFDuet-P yjhG -yjhG-dnaKJ-yqhD |
dnaKJ-yqhD-R | CTAAACGTCTCCACTATATATTCGGTCATCATGTGG | |
yqhD-dnaKJ-F | cgaatatatagtggagacgtttagGTAATGAACAACTTTAATCTGCACACCCCAACC | |
yqhD-dnaKJ-R | tcgacttaagcattatgcGAGGCGTAAAAAGCTTAGCGGGC | |
grpE-yqhD-F | cggcgcgcctgcaggGCCTTCCAGCACATCGGCTAACTGTTGC | pRSFDuet-P yjhG -yjhG-grpE-yqhD |
grpE-yqhD-R | GAATTTCTCCGCGTTTTTTTCGCATTCATCTC | |
yqhD-grpE-F | gaaaaaaacgcggagaaattcGTAATGAACAACTTTAATCTGCACACCCCAACC | |
yqhD-grpE-R | tcgacttaagcattatgcGAGGCGTAAAAAGCTTAGCGGGC | |
rpsA P1-xdh-F | aaatcataagcggccgcaTGAAAATTTTCCTTGACGCCTCCTCGG | pEtac-kivD-rpsA P1-xdh |
rpsA P1-xdh-R | gggatagatggctgaggacatgaaTTTGATCTCGGCCAAAAGGCGC | |
xdh-rpsA P1-F | gccgagatcaaaTTCATGTCCTCAGCCATCTATCCCCAGCTG | |
xdh-rpsA P1-R | tttgttagcagccggatcTCAACGCCAGCCGGCGTC | |
rpsT P1-xdh-F | aaatcataagcggccgcaTCATTGCCATGGCGCAAATCACGG | pEtac-kivD-rpsT P1-xdh |
rpsT P1-xdh-R | ggatagatggctgaggacatgaaACTCGTTACGTAGTGATGGCGCAG | |
xdh-rpsT P1-F | tacgtaacgagtTTCATGTCCTCAGCCATCTATCCCCAGCTG | |
xdh-rpsT P1-R | tttgttagcagccggatcTCAACGCCAGCCGGCGTC | |
inv-xdh-F | taagcggccgcaAACATTGAGGCAACGACAAGGTCGATTCTGCTATCCTTG AGGAGGCTCCTCTTCATGTCCTCAGCCATCTATCCC | pEtac-kivD-inv-xdh |
inv-xdh-R | tttgttagcagccggatcTCAACGCCAGCCGGCGTC | |
dnaKJ-xdh-F | aaatcataagcggccgcaTTTTTTGATGAATGCCTTGGCTGCGATTCATTC | pEtac-kivD-dnaKJ-xdh |
dnaKJ-xdh-R | CTAAACGTCTCCACTATATATTCGGTCATCATGTGGTTG | |
xdh-dnaKJ-F | cgaatatatagtggagacgtttagGAATTCATGTCCTCAGCCATCTATCCCCAG | |
xdh-dnaKJ-R | tttgttagcagccggatcTCAACGCCAGCCGGCGTC | |
grpE-xdh-F | aaatcataagcggccgcaAACGTTTCTCGCTGATGTAGTGGCC | pEtac-kivD-grpE-xdh |
grpE-xdh-R | GAATTTCTCCGCGTTTTTTTCGCATTCATCTCG | |
xdh-grpE-F | gaaaaaaacgcggagaaattcTTCATGTCCTCAGCCATCTATCCCCAGC | |
xdh-grpE-R | tttgttagcagccggatcTCAACGCCAGCCGGCGTC | |
rrnC P1-yjhG-F | aatttcacacaggaaacaCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCC | pRSFrrnC P1-yjhG-inv-yqhD |
rrnC P1-yjhG-R | gcaaaaatattgcgaacagacatgagTGGTGGCGCATTATAGGGAGTTATTCC | |
yjhG-rrnC P-F | aatgcgccaccaCTCATGTCTGTTCGCAATATTTTTGCTGACG | |
yjhG-rrnC P1-R | cctgcaggcgcgccgCTTTCAGTTTTTATTCATAAAATCGCGCAAAGCC | |
rrnH P1-yjhG-F | aatttcacacaggaaacaAAAGTGCTGGTGCGTACGGG | pRSFrrnH P1-yjhG-inv-yqhD |
rrnH P1-yjhG-R | caaaaatattgcgaacagacatgagTGGAGGCGCATTATAGGGAGTCGG | |
yjhG-rrnH P-F | gcgcctccaCTCATGTCTGTTCGCAATATTTTTGCTGACG | |
yjhG-rrnH P-R | cctgcaggcgcgccgCTTTCAGTTTTTATTCATAAAATCGCGCAAAGCC | |
tac-yjhG-F | aatttcacacaggaaacaGGAGCTTATCGACTGCACGGTGC | pRSFtac-yjhG-inv-yqhD |
tac-yjhG-R | TGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTC | |
yjhG-tac-F | gataacaatttcacacaggaaacaCTCATGTCTGTTCGCAATATTTTTGCTGACGAG | |
yjhG-tac-R | cctgcaggcgcgccgCTTTCAGTTTTTATTCATAAAATCGCGCAAAGCC | |
dnaKJ-rpsT-F | aatttcacacaggaaacaGCACAAAAAATTTTTGCATCTCCCCC | pRSFdnaKJrpsT-yjhG-inv-yqhD |
dnaKJ-rpsT-R | ttacgtagtgatggtATTCGGTCATCATGTGGTTGTGAG | |
rpsT-dnaKJ-F | ccacatgatgaccgaatACCATCACTACGTAACGAGTGCC | |
rpsT-dnaKJ-R | caaaaatattgcgaacagacatgagGGTCCAACTCCCAAATGTGTTCTATATGG | |
yjhG-KJT-F | agttggaccCTCATGTCTGTTCGCAATATTTTTGCTGACG | |
yjhG-KJT-R | cctgcaggcgcgccgCTTTCAGTTTTTATTCATAAAATCGCGCAAAGCC |
表2 本研究使用引物
引物名称 | 引物序列(5′→3′) | 质粒构建或功能 |
---|---|---|
Sg-yjhG-F | tcaaaaaggcatcaccacccGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTC | pTargetF-PMB(yjhG) |
Sg-yjhG-R | gggtggtgatgcctttttgaACTAGTATTATACCTAGGACTGAGCTAGCTGTCAAG | |
Sg-yqhD-F | gtgatctcccgtaaaaccacGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTC | pTargetF-PMB(yqhD) |
Sg-yqhD-R | gtggttttacgggagatcacACTAGTATTATACCTAGGACTGAGCTAGCTGTCAAG | |
dornor-yjhG-F | TGGCGGGAGATCGCCAGAGCTTCCGCGCGAGCTGCGCTGAACCTATATTGCGCC | 融合片段dornor(yjhG) |
dornor-yjhG-R | TCAATGGCGGTGGATTTGATCACCGAACCTTCTGGCGCAATATAGGTTCAGCGCAG | |
dornor-yqhD-F | TGTGTGCTGACGCTGCCAGCAACCGGTTCAGAATCCAACGCAGGCATCACTGAAGG | 融合片段dornor(yqhD) |
dornor-yqhD-R | GGCAATCGCGGCGTCAATACGCTCATCATCGGAACCTTCAGTGATGCCTGCGTTGG | |
yjhG-F | aatttcacacaggaaacaGTGATCCTGTACAACTTTCCGG | pRSFDuet-P yjhG -yjhG |
yjhG-R | cctgcaggcgcgccgGGTACCAGAAGAATTAAACCGACTTCG | |
yqhD-F | cggcgcgcctgcaggGTCCATCAACAGTTCCTGTAACTGC | pRSFDuet-P yjhG -yjhG-P yqhD-yqhD |
yqhD-R | tcgacttaagcattatgcAGTGGTGATGAACAGTTCTTCTCTG | |
tac-yqhD-F | cggcgcgcctgcaggGAGGGAGCTTATCGACTGCACGG | pRSFDuet-P yjhG -yjhG-tac-yqhD |
tac-yqhD-R | TGTTTCCTGTGTGAAATTGTTATCCGCTCAC | |
yqhD-tac-F | aatttcacacaggaaacaGAAGTAATGAACAACTTTAATCTGCACACCCC | |
yqhD-tac-R | tcgacttaagcattatgcGAGGCGTAAAAAGCTTAGCGGGC | |
inv-yqhD-F | cggcgcgcctgcaggAACATTGAGGCAACGACAAGGTCGATTCTGCTATCCTTG AGGAGGCTCCTCGTAATGAACAACTTTAATCTGCACACCCC | pRSF-P yjhG -yjhG-inv-yqhD |
inv-yqhD-R | tcgacttaagcattatgcGAGGCGTAAAAAGCTTAGCGGGC | |
dnaKJ-yqhD-F | cggcgcgcctgcaggCAGGAAACATTTTTTGATGAATGCCTTGGC | pRSFDuet-P yjhG -yjhG-dnaKJ-yqhD |
dnaKJ-yqhD-R | CTAAACGTCTCCACTATATATTCGGTCATCATGTGG | |
yqhD-dnaKJ-F | cgaatatatagtggagacgtttagGTAATGAACAACTTTAATCTGCACACCCCAACC | |
yqhD-dnaKJ-R | tcgacttaagcattatgcGAGGCGTAAAAAGCTTAGCGGGC | |
grpE-yqhD-F | cggcgcgcctgcaggGCCTTCCAGCACATCGGCTAACTGTTGC | pRSFDuet-P yjhG -yjhG-grpE-yqhD |
grpE-yqhD-R | GAATTTCTCCGCGTTTTTTTCGCATTCATCTC | |
yqhD-grpE-F | gaaaaaaacgcggagaaattcGTAATGAACAACTTTAATCTGCACACCCCAACC | |
yqhD-grpE-R | tcgacttaagcattatgcGAGGCGTAAAAAGCTTAGCGGGC | |
rpsA P1-xdh-F | aaatcataagcggccgcaTGAAAATTTTCCTTGACGCCTCCTCGG | pEtac-kivD-rpsA P1-xdh |
rpsA P1-xdh-R | gggatagatggctgaggacatgaaTTTGATCTCGGCCAAAAGGCGC | |
xdh-rpsA P1-F | gccgagatcaaaTTCATGTCCTCAGCCATCTATCCCCAGCTG | |
xdh-rpsA P1-R | tttgttagcagccggatcTCAACGCCAGCCGGCGTC | |
rpsT P1-xdh-F | aaatcataagcggccgcaTCATTGCCATGGCGCAAATCACGG | pEtac-kivD-rpsT P1-xdh |
rpsT P1-xdh-R | ggatagatggctgaggacatgaaACTCGTTACGTAGTGATGGCGCAG | |
xdh-rpsT P1-F | tacgtaacgagtTTCATGTCCTCAGCCATCTATCCCCAGCTG | |
xdh-rpsT P1-R | tttgttagcagccggatcTCAACGCCAGCCGGCGTC | |
inv-xdh-F | taagcggccgcaAACATTGAGGCAACGACAAGGTCGATTCTGCTATCCTTG AGGAGGCTCCTCTTCATGTCCTCAGCCATCTATCCC | pEtac-kivD-inv-xdh |
inv-xdh-R | tttgttagcagccggatcTCAACGCCAGCCGGCGTC | |
dnaKJ-xdh-F | aaatcataagcggccgcaTTTTTTGATGAATGCCTTGGCTGCGATTCATTC | pEtac-kivD-dnaKJ-xdh |
dnaKJ-xdh-R | CTAAACGTCTCCACTATATATTCGGTCATCATGTGGTTG | |
xdh-dnaKJ-F | cgaatatatagtggagacgtttagGAATTCATGTCCTCAGCCATCTATCCCCAG | |
xdh-dnaKJ-R | tttgttagcagccggatcTCAACGCCAGCCGGCGTC | |
grpE-xdh-F | aaatcataagcggccgcaAACGTTTCTCGCTGATGTAGTGGCC | pEtac-kivD-grpE-xdh |
grpE-xdh-R | GAATTTCTCCGCGTTTTTTTCGCATTCATCTCG | |
xdh-grpE-F | gaaaaaaacgcggagaaattcTTCATGTCCTCAGCCATCTATCCCCAGC | |
xdh-grpE-R | tttgttagcagccggatcTCAACGCCAGCCGGCGTC | |
rrnC P1-yjhG-F | aatttcacacaggaaacaCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCC | pRSFrrnC P1-yjhG-inv-yqhD |
rrnC P1-yjhG-R | gcaaaaatattgcgaacagacatgagTGGTGGCGCATTATAGGGAGTTATTCC | |
yjhG-rrnC P-F | aatgcgccaccaCTCATGTCTGTTCGCAATATTTTTGCTGACG | |
yjhG-rrnC P1-R | cctgcaggcgcgccgCTTTCAGTTTTTATTCATAAAATCGCGCAAAGCC | |
rrnH P1-yjhG-F | aatttcacacaggaaacaAAAGTGCTGGTGCGTACGGG | pRSFrrnH P1-yjhG-inv-yqhD |
rrnH P1-yjhG-R | caaaaatattgcgaacagacatgagTGGAGGCGCATTATAGGGAGTCGG | |
yjhG-rrnH P-F | gcgcctccaCTCATGTCTGTTCGCAATATTTTTGCTGACG | |
yjhG-rrnH P-R | cctgcaggcgcgccgCTTTCAGTTTTTATTCATAAAATCGCGCAAAGCC | |
tac-yjhG-F | aatttcacacaggaaacaGGAGCTTATCGACTGCACGGTGC | pRSFtac-yjhG-inv-yqhD |
tac-yjhG-R | TGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTC | |
yjhG-tac-F | gataacaatttcacacaggaaacaCTCATGTCTGTTCGCAATATTTTTGCTGACGAG | |
yjhG-tac-R | cctgcaggcgcgccgCTTTCAGTTTTTATTCATAAAATCGCGCAAAGCC | |
dnaKJ-rpsT-F | aatttcacacaggaaacaGCACAAAAAATTTTTGCATCTCCCCC | pRSFdnaKJrpsT-yjhG-inv-yqhD |
dnaKJ-rpsT-R | ttacgtagtgatggtATTCGGTCATCATGTGGTTGTGAG | |
rpsT-dnaKJ-F | ccacatgatgaccgaatACCATCACTACGTAACGAGTGCC | |
rpsT-dnaKJ-R | caaaaatattgcgaacagacatgagGGTCCAACTCCCAAATGTGTTCTATATGG | |
yjhG-KJT-F | agttggaccCTCATGTCTGTTCGCAATATTTTTGCTGACG | |
yjhG-KJT-R | cctgcaggcgcgccgCTTTCAGTTTTTATTCATAAAATCGCGCAAAGCC |
图2 敲除基因yjhG和yqhD的验证及对菌体生长和BT产量的影响M—2501 DNA marker;1—PCR验证菌株KXW3009中基因yjhG;2—PCR验证菌株KXW3010中基因yjhG;3—PCR验证菌株KXW3009中基因yqhD;4—PCR验证菌株KXW3010中基因yqhD;KXW3009—E. coli W3009, pEtac-kivD-tac-xdh;KXW3010—E. coli W3010, pEtac-kivD-tac-xdh
图3 重组菌中YqhD酶活菌株KXW3009—pEtac-kivD-tac-xdh;菌株P1—pRSFDuet-P yjhG -yjhG-P yqhD-yqhD;菌株P2—pRSFDuet-P yjhG -yjhG-P tac-yqhD;菌株P3—pRSFDuet-P yjhG -yjhG-P inv-yqhD;菌株P4—pRSFDuet-P yjhG -yjhG-P dnaKJ-yqhD;菌株P5—pRSFDuet-P yjhG -yjhG-P grpE-yqhD
菌株 | 启动子(yqhD) | OD600 | 木糖转化率/% | BT/g·L-1 |
---|---|---|---|---|
KXW3009 | 未改造 | 13.9 | 63.3 | 13.6 |
P1 | P yqhD (野生型启动子) | 15.4 | 58.3 | 9.1 |
P2 | P tac | 13.1 | 64.0 | 10.2 |
P3 | P inv | 10.4 | 73.1 | 14.4 |
P4 | P dnaKJ | 12.0 | 66.4 | 14.2 |
P5 | P grpE | 11.5 | 70.0 | 14.1 |
表3 不同强度醇脱氢酶表达菌株在48 h时的发酵参数
菌株 | 启动子(yqhD) | OD600 | 木糖转化率/% | BT/g·L-1 |
---|---|---|---|---|
KXW3009 | 未改造 | 13.9 | 63.3 | 13.6 |
P1 | P yqhD (野生型启动子) | 15.4 | 58.3 | 9.1 |
P2 | P tac | 13.1 | 64.0 | 10.2 |
P3 | P inv | 10.4 | 73.1 | 14.4 |
P4 | P dnaKJ | 12.0 | 66.4 | 14.2 |
P5 | P grpE | 11.5 | 70.0 | 14.1 |
菌株 | 启动子(xdh) | 启动子(yjhG) | BT/g·L-1 | OD600 | 木糖消耗率/% | 菌株 | 启动子(xdh) | 启动子(yjhG) | BT/g·L-1 | OD600 | 木糖消耗率/% |
---|---|---|---|---|---|---|---|---|---|---|---|
P3 | P tac | P yjhG | 14.1 | 12.2 | 63.8 | P34 | P inv | P rrnC P1 | 3.2 | 8.0 | 22.4 |
P12 | P rpsA P1 | P yjhG | 9.2 | 9.9 | 44.5 | P35 | P dnaKJ | P rrnC P1 | 6.7 | 14.3 | 36.7 |
P13 | P rpsT P1 | P yjhG | 9.6 | 11.1 | 40.8 | P36 | P grpE | P rrnC P1 | 5.1 | 11.6 | 40.9 |
P14 | P inv | P yjhG | 11.4 | 9.2 | 47.9 | P41 | P tac | P rrnH P1 | 15.6 | 12.3 | 65.7 |
P15 | P dnaKJ | P yjhG | 10.1 | 11.4 | 40.4 | P42 | P rpsA P1 | P rrnH P1 | 7.7 | 10.6 | 24.4 |
P16 | P grpE | P yjhG | 5.8 | 11.8 | 35.9 | P43 | P rpsT P1 | P rrnH P1 | 8.1 | 10.8 | 25.0 |
P21 | P tac | P tac | 7.6 | 12.0 | 47.3 | P44 | P inv | P rrnH P1 | 10.2 | 9.6 | 48.1 |
P22 | P rpsA P1 | P tac | 8.2 | 9.5 | 24.6 | P45 | P dnaKJ | P rrnH P1 | 8.0 | 13.4 | 28.8 |
P23 | P rpsT P1 | P tac | 4.4 | 11.2 | 19.6 | P46 | P grpE | P rrnH P1 | 5.23 | 10.5 | 34.2 |
P24 | P inv | P tac | 11.1 | 9.2 | 47.7 | P51 | P tac | P dnaKJrpsT | 9.6 | 12.7 | 43.0 |
P25 | P dnaKJ | P tac | 7.4 | 12.1 | 27.2 | P52 | P rpsA P1 | P dnaKJrpsT | 6.2 | 9.4 | 24.0 |
P26 | P grpE | P tac | 5.0 | 11.1 | 33.5 | P53 | P rpsT P1 | P dnaKJrpsT | 5.7 | 11.7 | 21.9 |
P31 | P tac | P rrnC P1 | 6.4 | 12.9 | 29.3 | P54 | P inv | P dnaKJrpsT | 2.9 | 13.0 | 17.0 |
P32 | P rpsA P1 | P rrnC P1 | 6.7 | 9.6 | 30.4 | P55 | P dnaKJ | P dnaKJrpsT | 7.8 | 12.2 | 25.2 |
P33 | P rpsT P1 | P rrnC P1 | 5.2 | 12.4 | 35.9 | P56 | P grpE | P dnaKJrpsT | 5.7 | 10.8 | 35.9 |
表4 不同菌株在48h时的发酵参数
菌株 | 启动子(xdh) | 启动子(yjhG) | BT/g·L-1 | OD600 | 木糖消耗率/% | 菌株 | 启动子(xdh) | 启动子(yjhG) | BT/g·L-1 | OD600 | 木糖消耗率/% |
---|---|---|---|---|---|---|---|---|---|---|---|
P3 | P tac | P yjhG | 14.1 | 12.2 | 63.8 | P34 | P inv | P rrnC P1 | 3.2 | 8.0 | 22.4 |
P12 | P rpsA P1 | P yjhG | 9.2 | 9.9 | 44.5 | P35 | P dnaKJ | P rrnC P1 | 6.7 | 14.3 | 36.7 |
P13 | P rpsT P1 | P yjhG | 9.6 | 11.1 | 40.8 | P36 | P grpE | P rrnC P1 | 5.1 | 11.6 | 40.9 |
P14 | P inv | P yjhG | 11.4 | 9.2 | 47.9 | P41 | P tac | P rrnH P1 | 15.6 | 12.3 | 65.7 |
P15 | P dnaKJ | P yjhG | 10.1 | 11.4 | 40.4 | P42 | P rpsA P1 | P rrnH P1 | 7.7 | 10.6 | 24.4 |
P16 | P grpE | P yjhG | 5.8 | 11.8 | 35.9 | P43 | P rpsT P1 | P rrnH P1 | 8.1 | 10.8 | 25.0 |
P21 | P tac | P tac | 7.6 | 12.0 | 47.3 | P44 | P inv | P rrnH P1 | 10.2 | 9.6 | 48.1 |
P22 | P rpsA P1 | P tac | 8.2 | 9.5 | 24.6 | P45 | P dnaKJ | P rrnH P1 | 8.0 | 13.4 | 28.8 |
P23 | P rpsT P1 | P tac | 4.4 | 11.2 | 19.6 | P46 | P grpE | P rrnH P1 | 5.23 | 10.5 | 34.2 |
P24 | P inv | P tac | 11.1 | 9.2 | 47.7 | P51 | P tac | P dnaKJrpsT | 9.6 | 12.7 | 43.0 |
P25 | P dnaKJ | P tac | 7.4 | 12.1 | 27.2 | P52 | P rpsA P1 | P dnaKJrpsT | 6.2 | 9.4 | 24.0 |
P26 | P grpE | P tac | 5.0 | 11.1 | 33.5 | P53 | P rpsT P1 | P dnaKJrpsT | 5.7 | 11.7 | 21.9 |
P31 | P tac | P rrnC P1 | 6.4 | 12.9 | 29.3 | P54 | P inv | P dnaKJrpsT | 2.9 | 13.0 | 17.0 |
P32 | P rpsA P1 | P rrnC P1 | 6.7 | 9.6 | 30.4 | P55 | P dnaKJ | P dnaKJrpsT | 7.8 | 12.2 | 25.2 |
P33 | P rpsT P1 | P rrnC P1 | 5.2 | 12.4 | 35.9 | P56 | P grpE | P dnaKJrpsT | 5.7 | 10.8 | 35.9 |
图5 重组菌中Xdh和YqhD的酶活及SDS-PAGE电泳分析M—蛋白marker;菌株KXW3009—pEtac-kivD-tac-xdh;菌株P41—pRSFrrnH P1-yjhG-inv-yqhD, pEtac-kivD-tac-xdh;菌株P24—pRSFtac-yjhG-inv-yqhD, pEtac-kivD-inv-xdh;菌株P14—pRSF-P yjhG -yjhG-inv-yqhD, pEtac-kivD-inv-xdh;菌株W3010—菌株KXW3009 ΔyjhGΔyqhD
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