伪蛋白生物材料的分类、合成及其应用

doi:10.16085/j.issn.1000-6613.2023-0626

摘要/Abstract

摘要：

伪蛋白是一种新型合成生物可降解材料，以氨基酸为基本结构单元，它与蛋白质不同的是除了含有肽键外，还含有其他连接基团，这种结构使其既具有蛋白质的优良特性，又具有良好的力学性能、热性能、可调节的物理化学性能以及优异的细胞相容性和3D微孔结构等性质，因此伪蛋白能够有效应用于载体、伤口愈合、组织工程等生物医药领域。本文首先综述了目前所研究的伪蛋白的类型，包括非功能性和功能性伪蛋白两类；之后详细阐述了缩聚合成伪蛋白的方法，包括溶液缩聚、界面聚合、熔融缩聚、开环聚合四种方法；接着归纳了伪蛋白生物材料在药物载体、基因载体、组织工程、创面敷料等方面的应用；最后，对伪蛋白生物材料在合成路线及应用方面未来的发展进行了探讨和展望。

关键词: 伪蛋白, 合成, 聚合物, 载体, 组织工程

Abstract:

Pseudo-protein is a new synthetic biodegradable material. It takes amino acids as the basic structural unit. Unlike protein, pseudo-protein has other linking groups in addition to peptide bonds, which gives it not only the excellent properties of proteins, but also the good mechanical properties, thermal properties, adjustable physicochemical properties, excellent cytocompatibility, 3D microporous structure, etc. Therefore, pseudo-proteins can be effectively applied in carriers, wound healing, tissue engineering, etc. Firstly, this paper reviews the types of pseudo-proteins studied so far, including non-functional and functional pseudo-proteins, followed by a detailed description of the methods for the synthesis of pseudo-proteins by condensation, including solution polycondensation, interfacial polymerization, melt polycondensation and ring-opening polymerization, and then summarizes the applications of pseudo-protein biomaterials in drug carrier, gene carrier, tissue engineering and wound dressing. Finally, the future development of synthetic routes and applications of pseudo-protein biomaterials is prospected.

Key words: pseudo-protein, synthesis, polymers, carrier, tissue engineering

中图分类号:

O621.3

薛云娇, 张璇, 刘洋, 陈玉焕, 房静, 杨芳. 伪蛋白生物材料的分类、合成及其应用[J]. 化工进展, 2024, 43(4): 2001-2016.

XUE Yunjiao, ZHANG Xuan, LIU Yang, CHEN Yuhuan, FANG Jing, YANG Fang. Pseudo-protein biomaterials: Classification, synthesis and application[J]. Chemical Industry and Engineering Progress, 2024, 43(4): 2001-2016.

图/表 16

参考文献 104

1	ABBAS Manzar, ZOU Qianli, LI Shukun, et al. Self-assembled peptide- and protein-based nanomaterials for antitumor photodynamic and photothermal therapy[J]. Advanced Materials, 2017, 29(12): 1605021.
2	DEFRATES Kelsey G, MOORE Robert, BORGESI Julia, et al. Protein-based fiber materials in medicine: A review[J]. Nanomaterials, 2018, 8(7): 457.
3	MISAWA Nobuo, OSAKI Toshihisa, TAKEUCHI Shoji. Membrane protein-based biosensors[J]. Journal of the Royal Society‍, Interface, 2018, 15(141): 20170952.
4	FERRIS Cristina, VIOLANTE DE PAZ M, ZAMORA Francisca, et al. Dithiothreitol-based polyurethanes. Synthesis and degradation studies[J]. Polymer Degradation and Stability, 2010, 95(9): 1480-1487.
5	RUANO Guillem, Angélica DíAZ, TONONI Jordi, et al. Biohydrogel from unsaturated polyesteramide: Synthesis, properties and utilization as electrolytic medium for electrochemical supercapacitors[J]. Polymer Testing, 2020, 82: 106300.
6	IKADA Yoshito, TSUJI Hideto. Biodegradable polyesters for medical and ecological applications[J]. Macromolecular Rapid Communications, 2000, 21(3): 117-132.
7	HE Mingyu, POTUCK Alicia, ZHANG Yi, et al. Arginine-based polyester amide/polysaccharide hydrogels and their biological response[J]. Acta Biomaterialia, 2014, 10(6): 2482-2494.
8	HE Mingyu, POTUCK Alicia, KOHN Julie C, et al. Self-assembled cationic biodegradable nanoparticles from pH-responsive amino-acid-based poly(ester urea urethane)s and their application as a drug delivery vehicle[J]. Biomacromolecules, 2016, 17(2): 523-537.
9	CHU C C. Novel biodegradable functional amino acid-based poly(ester amide) biomaterials: Design, synthesis,property and biomedical applications[J]. Journal of Fiber Bioengineering and Informatics, 2018, 5(1): 1-31.
10	HORWITZ Joshua A, SHUM Katrina M, BODLE Josephine C, et al. Biological performance of biodegradable amino acid-based poly(ester amide)s: Endothelial cell adhesion and inflammation in vitro[J]. Journal of Biomedical Materials Research A, 2010, 95A(2): 371-380.
11	YIN Maoli, WAN Shuangshuang, REN Xuehong, et al. Development of inherently antibacterial, biodegradable, and biologically active chitosan/pseudo-protein hybrid hydrogels as biofunctional wound dressings[J]. ACS Applied Materials & Interfaces, 2021, 13(12): 14688-14699.
12	KATSARAVA R, BERIDZE V, ARABULI N, et al. Amino acid based bioanalogous polymers. Synthesis and study of regular poly (ester amide)s based on bis(a-amino acid) α, ω-alkylene diesters and aliphatic dicarboxylic acids[J]. Journal of Polymer Science A: Polymer Chemistry, 1999, 37: 391-407.
13	CHU Chih-Chang, KATSARAVA R. Elastomeric functional biodegradable copolyester amides and copolyester urethanes: US7408018[P]. 2008-08-05.
14	HE Mingyu, CHU Chih-Chang. A new family of functional biodegradable arginine-based polyester urea urethanes: Synthesis, chracterization and biodegradation[J]. Polymer, 2013, 54(16): 4112-4125.
15	WU Jun, WU Dequn, MUTSCHLER Martha A, et al. Cationic hybrid hydrogels from amino-acid-based poly(ester amide): Fabrication, characterization, and biological properties[J]. Advanced Functional Materials, 2012, 22(18): 3815-3823.
16	WU Jun, MUTSCHLER Martha A, CHU Chih-Chang. Synthesis and characterization of ionic charged water soluble arginine-based poly (ester amide) [J]. Journal of Materials Science Materials in Medicine, 2011, 22(3): 469-479.
17	DENG Mingxiao, WU Jun, REINHART-KING Cynthia A, et al. Synthesis and characterization of biodegradable poly(ester amide)s with pendant amine functional groups and in vitro cellular response[J]. Biomacromolecules, 2009, 10(11): 3037-3047.
18	GUO Kai, CHU Chih-Chang. Synthesis and characterization of poly(ε‍-caprolactone) containing amino acid-based poly(ether ester amide)s[J]. Journal of Applied Polymer Science, 2012, 125(1): 812-819.
19	YAO Kaili, LIU Zhanli, LI Ting, et al. Mesoscale structure-based investigation of polyurea dynamic modulus and shock-wave dissipation[J]. Polymer, 2020, 202: 122741.
20	FAN Jitang, CHEN Ang. Studying a flexible polyurethane elastomer with improved impact-resistant performance[J]. Polymers, 2019, 11(3): 467.
21	GUO Kai, CHU Chih-Chang. Copolymers of unsaturated and saturated poly(ether ester amide)s: Synthesis, characterization, and biodegradation[J]. Journal of Applied Polymer Science, 2008, 110(3): 1858-1869.
22	GUO Kai, CHU Chih-Chang. Synthesis, characterization, and biodegradation of copolymers of unsaturated and saturated poly(ester amide)s[J]. Journal of Polymer Sciencet A: Polymer Chemistry, 2007,45(9): 1595-1606.
23	GUO Kai, CHU Chih-Chang. Biodegradation of unsaturated poly(ester-amide)s and their hydrogels[J]. Biomaterials, 2007, 28(22): 3284-3294.
24	GUO Kai, CHU Chih-Chang. Controlled release of hydrophobic drugs from biodegradable unsaturated poly(ester amide)s/poly(ethylene glycol) diacrylate hydrogels[J]. Journal of Biomaterials Science, Polymer Edition, 2007, 18(5): 489-504.
25	TANG Lin, SHAO Shuren, WANG Ao, et al. Influence of fluorocarbon side chain on microphase separation and chemical stability of silicon-containing polycarbonate urethane[J]. Polymer, 2022, 242: 124538.
26	XU Cancan, HONG Yi. Rational design of biodegradable thermoplastic polyurethanes for tissue repair[J]. Bioactive Materials, 2022, 15: 250-271.
27	PANG Xuan, WU Jun, CHU Chih-Chang, et al. Development of an arginine-based cationic hydrogel platform: Synthesis, characterization and biomedical applications[J]. Acta Biomaterialia, 2014, 10(7): 3098-3107.
28	PANG Xuan, WU Jun, REINHART-KING C, et al. Synthesis and characterization of functionalized water soluble cationic poly(ester amide)s[J]. Journal of Polymer Science A: Polymer Chemistry, 2010, 48(17): 3758-3766.
29	JOKHADZE G, MACHAIDZE M, PANOSYAN H, et al. Synthesis and characterization of functional elastomeric poly(ester amide) co-polymers[J]. Biomaterials Science Polymer Edition, 2007, 18(4): 411-438.
30	PAREDES N, RODRÍGUEZ-GALÁN A, PUIGGALI J. Synthesis and characterization of a family of biodegradable poly(ester amide)s derived from glycine[J]. Journal of Polymer Science A: Polymer Chemistry, 1998, 36(8): 1271-1282.
31	ABDOLMALEKI Amir, MALLAKPOUR Shadpour, ESMAELI Ramtin Nazari, et al. Synthesis and structural characterization of novel nanostructured aromatic optically active poly(ester-amide)s derived from S-tyrosine containing symmetric diol and aromatic diacid chlorides[J]. Polymer-Plastics Technology and Engineering, 2016, 55(9): 911-919.
32	ABDOLMALEKI Amir, MALLAKPOUR Shadpour, BORANDEH Sedigheh. Effect of silane-modified ZnO on morphology and properties of bionanocomposites based on poly(ester-amide) containing tyrosine linkages[J]. Polymer Bulletin, 2012, 69: 15-28.
33	ATKINS Katelyn M, LOPEZ David, KNIGHT Darryl K, et al. A versatile approach for the syntheses of poly(ester amide)s with pendant functional groups[J]. Journal of Polymer Science A: Polymer Chemistry, 2009, 47(15): 3757-3772.
34	VERA Montserrat, FRANCO Lourdes, PUIGGALI Jordi. Synthesis of poly(ester amide)s with lateral groups from a bulk polycondensation reaction with formation of sodium chloride salts[J]. Journal of Polymer Science A: Polymer Chemistry, 2008, 46(2): 661-667.
35	Alfonso RODRIGUEZ-GALAN, FRANCO Lourdes, PUIGGALI Jordi. Degradable poly(ester amide)s for biomedical applications[J]. Polymers, 2010, 3(1): 65-99.
36	GUO Kai, CHU Chih-Chang, CHKHAIDZE E, et al. Synthesis and characterization of novel biodegradable unsaturated poly(ester amide)s[J]. Journal of Polymer Science A: Polymer Chemistry, 2005, 43(7): 1463-1477.
37	TSITLANADZE G, MACHAIDZE M, KVIRIA T, et al. Biodegradation of amino-acid-based poly(ester amide)s: In vitro weight loss and preliminary in vivo studies[J]. Journal of Biomaterials Science, Polymer Edition, 2004, 15(1): 1-24.
38	GUO Kai, CHU Chih-Chang. Synthesis of biodegradable amino-acid-based poly(ester amide)s and poly(ether ester amide)s with pendant functional groups[J]. Journal of Applied Polymer Science, 2010, 117(6): 3386-3394.
39	SONG H, CHU Chih-Chang. Synthesis and characterization of a new family of cationic amino acid-based poly(ester amide)s and their biological properties[J]. Journal of Applied Polymer Science, 2012, 124(5): 3840-3853.
40	GUO Kai, CHU Chih-Chang. Synthesis, characterization, and biodegradation of novel poly(ether ester amide)s based on l-phenylalanine and oligoethylene glycol[J]. Biomacromolecules, 2007, 8(9): 2851-2861.
41	YU Jiayi, LIN Fei, LIN Panpan, et al. Phenylalanine-based poly(ester urea): Synthesis, characterization, and in vitro degradation[J]. Macromolecules, 2014, 47(1): 121-129.
42	LI Mengna, LI Na, QIU Weiwang, et al. Phenylalanine-based poly(ester urea)s composite films with nitric oxide-releasing capability for anti-biofilm and infected wound healing applications[J]. Journal of Colloid and Interface Science, 2022, 607: 1849-1863.
43	PANG Xuan, CHU Chih-Chang. Synthesis, characterization and biodegradation of functionalized amino acid-based poly(ester amide)s[J]. Biomaterials 2010, 31(14): 3745-3754.
44	HE Mingyu, Lillian RO, LIU Jing, et al. Folate-decorated arginine-based poly(ester urea urethane) nanoparticles as carriers for gambogic acid and effect on cancer cells[J]. Journal of Biomedical Materials Research A, 2017, 105(2): 475-490.
45	DE WIT Matthew A, WANG Zixi, ATKINS Katelyn M, et al. Syntheses, characterization, and functionalization of poly(ester amide)s with pendant amine functional groups[J]. Journal of Polymer Science A: Polymer Chemistry, 2008, 46(19): 6376-6392.
46	HE Pan, TANG Zhaohui, LIN Lin, et al. Novel biodegradable and pH-sensitive poly(ester amide) microspheres for oral insulin delivery[J]. Macromolecular Bioscience, 2012, 12(4): 547-556.
47	ODIAN G. Principles of polymerization[M]. New Jersey: John Wiley & Sons Inc, 2004: 832.
48	WITTBECKER Emerson L, MORGAN Paul W. Interfacial polycondensation. Ⅰ. The formation of surface graft polymers on wool [J]. Journal of Polymer Science, 1959, 40(137): 289-297.
49	MORGAN Paul W, KWOLEK Stephanie L. Interfacial polycondensation: Ⅱ. Fundamentals of polymer formation at liquid interfaces[J]. Journal of Polymer Science, 1959, 40(137): 299–327.
50	KARIMI Pooneh, RIZKALLA Amin S, MEQUANINT Kibret. Versatile biodegradable poly(ester amide)s derived from α‍-amino acids for vascular tissue engineering[J]. Materials, 2010, 3(4): 2346-2368.
51	NATARAJAN Janeni, CHATTERJEE Kaushik, MADRAS Giridhar. Tailored degradation and dye released from poly(ester amides)[J]. Polymer-Plastics Technology and Engineering, 2017, 56(6): 635-646.
52	AL-TAYYEM Ban H, SWEILEH Bassam A. Synthesis, characterization and hydrolytic degradation of novel biodegradable poly(ester amide)s derived from Isosorbide and α-amino acids[J]. Journal of Polymer Research, 2020, 27: 120.
53	GAO Yaohua, CHILDERS Erin P, BECKER Matthew L. L-leucine-based poly(ester urea)s for vascular tissue engineering[J]. ACS Biomaterials Science & Engineering, 2015, 1(19): 795-804.
54	PETERSON Gregory I, CHILDERS Erin P, LI Hao, et al. Tunable shape memory polymers from α-amino acid-based poly(ester urea)s[J]. Macromolecules, 2017, 50(11): 4300-4308.
55	DREGER Nathan Z, FAN Zhaobo, ZANDER Zachary K, et al. Amino acid-based poly(ester urea) copolymer films for hernia-repair applications[J]. Biomaterials, 2018, 182: 44-57.
56	POLICASTRO Gina M, LIN Fei, SMITH CALLAHAN Laura A, et al. OGP functionalized phenylalanine-based poly(ester urea) for enhancing osteoinductive potential of human mesenchymal stem cells[J]. Biomacromolecules, 2015, 16(4): 1358-1371.
57	DE JONGH Patrick A J M, PAUL Prem K C, KHOSHDEL Ezat, et al. High T_g poly(ester amide)s by melt polycondensation of monomers from renewable resources; citric acid, D-glucono-δ-lactone and amino acids: A DSC study[J]. European Polymer Journal, 2017, 94: 11-19.
58	ASÍN L, ARMELIN E, MONTANÉ J, et al. Sequential poly(ester amide)s based on glycine, diols, and dicarboxylic acids: Thermal polyesterification versus interfacial polyamidation. Characterization of polymers containing stiff units[J]. Journal of Polymer Science A: Polymer Chemistry, 2001, 39(24): 4283-4293.
59	ABBES Marwa, SALHI Slim, LEFEVRE Lénaïg, et al. Poly(ester-amide)s derived from adipic acid, 1,4-butanediol and β-alanine[J]. Journal of Macromolecular Science A, 2015, 52(1): 56-63.
60	GUPTA Sachchidanand Soaham, MISHRA Vivek, MUKHERJEE Maumita Das, et al. Amino acid derived biopolymers: Recent advances and biomedical applications[J]. International Journal of Biological Macromolecules, 2021, 188: 542-567.
61	SUN Huanli, MENG Fenghua, DIAS Aylvin A, et al. α-Amino acid containing degradable polymers as functional biomaterials: Rational design, synthetic pathway, and biomedical applications[J]. Biomacromolecules, 2011, 12(6): 1937-1955.
62	IN’T VELD Peter J A, DIJKSTRA Pieter J, FEIJEN Jan. Synthesis of biodegradable polyesteramides with pendant functional groups[J]. Die Macromolekulare Chemie, 1992, 193(11): 2713-2730.
63	DENG Mingxiao, WU Jun, REINHART-KING Cynthia A, et al. Biodegradable functional poly(ester amide)s with pendant hydroxyl functional groups: Synthesis, characterization, fabrication and in vitro cellular response[J]. Acta Biomaterialia, 2011, 7(4): 1504-1515.
64	KNIGHT Darryl K, GILLIES Elizabeth R, MEQUANINT Kibret. Biomimetic L-aspartic acid-derived functional poly(ester amide)s for vascular tissue engineering[J]. Acta Biomaterialia, 2014, 10(8): 3484-3496.
65	VILLAMAGNA Ian J, GORDON Trent N, HURTIG Mark B, et al. Poly(ester amide) particles for controlled delivery of celecoxib[J]. Journal of Biomedical Materials Research A, 2019, 107(6):1235-1243.
66	GUO Kai, CHU Chih-Chang. Biodegradable and injectable paclitaxel-loaded poly(ester amide)s microspheres: Fabrication and characterization[J]. Journal of Biomedical Materials Research B: Applied Biomaterials, 2009, 89: 491-500.
67	MEMANISHVILI Tamar, ZAVRADASHVILI Nino, KUPATADZE Nino, et al. Arginine-based biodegradable ether-ester polymers with low cytotoxicity as potential gene carriers[J]. Biomacromolecules, 2014, 15(8): 2839-2848.
68	LIU Jing, WANG Pei, CHU Chih-Chang, et al. Arginine-leucine based poly (ester urea urethane) coating for Mg-Zn-Y-Nd alloy in cardiovascular stent applications[J]. Colloids and Surfaces B: Biointerfaces, 2017, 159(1): 78-88.
69	CAO Kai, FLEGG Derek S, LIN Shigang, et al. Fabrication and in situ cross-linking of carboxylic-acid-functionalized poly(ester amide) scaffolds for tissue engineering[J]. ACS Applied Polymer Materials, 2019, 1(9): 2360-2369.
70	JI Ying, SHAN Shuo, HE Mingyu, et al. A novel pseudo-protein-based biodegradable nanomicellar platform for the delivery of anticancer drugs[J]. Small, 2017, 13(1): 1601491.
71	ZHU Jie, HAN Hua, LI Faxue, et al. Self-assembly of amino acid-based random copolymers for antibacterial application and infection treatment as nanocarriers[J]. Journal of Colloid and Interface Science, 2019, 540: 634-646.
72	LANE D D, SU F Y, CHIU D Y, et al. Dynamic intracellular delivery of antibiotics via pH-responsive polymersomes[J]. Polymer Chemistry, 2015, 6(8): 1255-1266.
73	SADEGHI Ilin, KRONENBERG Jacob, ASATEKIN Ayse. Selective transport through membranes with charged nanochannels formed by scalable self-assembly of random copolymer micelles[J]. ACS Nano, 2018,12(1): 95-108.
74	KWAN Hiu Yee, XU Qinghua, GONG Ruihong, et al. Targeted Chinese medicine delivery by a new family of biodegradable pseudo-protein nanoparticles for treating triple-negative breast cancer: In vitro and in vivo study[J]. Frontiers in Oncology, 2020, 10: 600298.
75	JI Ying, ZHAO Jihui, CHU Chih-Chang. Biodegradable nanocomplex from hyaluronic acid and arginine based poly(ester amide)s as the delivery vehicles for improved photodynamic therapy of multidrug resistant tumor cells[J]. Journal of Biomedical Materials Research A, 2017, 105(5): 1487-1499.
76	YU Jianling, YANG Fang, LIU Zhihua, et al. Preparation and characterization of C₁₀-C₁₄ alkyl cellulose ester sulfate surfactant[J]. Journal of Surfactants and Detergents, 2014, 17(4): 647-653.
77	YANG Fang, LIU Yanan, YU Jianling, et al. Synthesis, micellization behavior and alcohol induced amphipathic cellulose film of cellulose-based amphiphilic surfactant[J]. Applied Surface Science, 2015, 345(1): 187-193.
78	YANG Fang, HAN Huaxin, FAN Hongxian, et al. Synthesis, characterization, and in vitro release analysis of a novel glucan based polymer carrier[J]. Colloid and Polymer Science, 2018, 296(8): 1401-1407.
79	YANG Fang, XIAO Dan, HAN Huaxin, et al. Amphiphilic polymeric micelles originating from 1,4,β,D-glucan-g-polyphenylene oxide as the carriers for delivery of docetaxel and the corresponding release behaviors[J]. International Journal of Biological Macromolecules, 2018, 114: 194-203.
80	WU Dequn, WU Jun, CHU Chih-Chang. A novel family of biodegradable hybrid hydrogels from arginine-based poly(ester amide) and hyaluronic acid precursors[J]. Soft Matter, 2013, 9(15): 3965-3975.
81	HOFFMAN A S. Hydrogels for biomedical applications[J]. Advanced Drug Delivery Reviews, 2012, 64: 18-23.
82	HERNANDEZ-GONZALEZ Aurora C, Lucía TELLEZ-JURADO, RODRIGUEZ-LORENZO Luis M. Alginate hydrogels for bone tissue engineering, from injectables to bioprinting: A review[J]. Carbohydrate Polymers, 2019, 229: 115514.
83	YANG Jun, SHEN Mingyue, WEN Huiliang, et al. Recent advance in delivery system and tissue engineering applications of chondroitin sulfate[J]. Carbohydrate Polymers, 2020, 230: 115650.
84	ZHANG S H, XIN P K, OU Q M, et al. Poly(ester amide)-based hybrid hydrogels for efficient transdermal insulin delivery[J]. Journal of Materials Chemistry B, 2018, 6(42): 6723-6730.
85	SINHA V R, TREHAN A. Biodegradable microspheres for parenteral delivery[J]. Critical Reviews In Therapeutic Drug Carrier Systems, 2005, 22(6): 535-602.
86	TAMBER H, JOHANSEN P, MERKLE H P, et al. Formulation aspects of biodegradable polymeric microspheres for antigen delivery[J]. Advanced Drug Delivery Reviews, 2005, 57(3): 357-376
87	NALDINI Luigi. Gene therapy returns to centre stage[J]. Nature, 2015, 526 (7573): 351-360.
88	SCHAFFERT D, WAGNER E. Gene therapy progress and prospects: Synthetic polymer-based systems[J]. Gene Therapy, 2008, 15 (16): 1131-1138.
89	YOU Xinru, GU Zhipeng, HUANG Jun, et al. Arginine-based poly(ester amide) nanoparticle platform: From structure-property relationship to nucleic acid delivery[J]. Acta Biomaterialia, 2018, 74:180-191.
90	DIAZ Angélica, DEL VALLE Luis J, TUGUSHI David, et al. New poly(ester urea) derived from L-leucine: Electrospun scaffolds loaded with antibacterial drugs and enzymes[J]. Materials Science & Engineering C, 2015, 46: 450-462.
91	SADAVA Emmanuel E, KRPATA David M, GAO Yue, et al. Wound healing process and mediators: Implications for modulations for hernia repair and mesh integration[J]. Journal of Biomedical Materials Research A, 2014, 102(1): 295-302.
92	ZHU Leiming, SCHUSTER Philipp, KLINGE Uwe. Mesh implants: An overview of crucial mesh parameters[J]. World Journal of Gastrointestinal Surgery, 2015, 7(10): 226-236.
93	DREGER Nathan Z, WANDEL Mary B, ROBINSON Lindsay L, et al. Preclinical in vitro and in vivo assessment of linear and branched L-valine-based poly(ester urea)s for soft tissue applications[J]. ACS Biomaterials Science & Engineering, 2018, 4(4): 1346-1356.
94	WITTE Frank. The history of biodegradable magnesium implants: A review[J]. Acta Biomaterialia, 2010, 6(5): 1680-1692.
95	WITTE Frank, HORT Norbert, VOGT Carla, et al. Degradable biomaterials based on magnesium corrosion[J]. Current Opinion in Solid State and Materials Science, 2008, 12(5/6): 63-72.
96	WANG Juan, HE Yonghui, MAITZ Manfred F, et al. A surface-eroding poly(1,3-trimethylene carbonate) coating for fully biodegradable magnesium-based stent applications: Toward better biofunction, biodegradation and biocompatibility[J]. Acta Biomaterialia, 2013, 9(10): 8678-8689.
97	LIU J, LIU X L, XI T F, et al. A novel pseudo-protein-based biodegradable coating for magnesium substrate: in vitro corrosion phenomena and cytocompatibility[J]. Journal of Materials Chemistry B, 2015, 3(5): 878-893.
98	WENDELS Sophie, AVEROUS Luc. Biobased polyurethanes for biomedical applications[J]. Bioactive Materials, 2021, 6(4): 1083-1106.
99	EMING Sabine A, WYNN Thomas A, MARTIN Paul. Inflammation and metabolism in tissue repair and regeneration[J]. Science, 2017, 356(6342): 1026-1030.
100	LU Lan, HU Wei, TIAN Zeru, et al. Developing natural products as potential anti-biofilm agents[J]. Chinese Medicine, 2019, 14(1): 11.
101	VERDEROSA Anthony D, TOTSIKA Makrina, Makrina FAIRFULL-SMITH. Bacterial biofilm eradication agents: A current review[J]. Frontiers in Chemistry, 2019, 7: 824.
102	ABEL Alexandra K, DREGER Nathan Z, NETTLETON Karissa, et al. Amino acid-based poly(ester urea)s as a matrix for extended release of entecavir[J]. Biomacromolecules, 2020, 21(2): 946-954.
103	BRIGHAM Natasha C, NOFSINGER Rebecca, LUO Xin, et al. Controlled release of etoricoxib from poly(ester urea) films for post-operative pain management[J]. Journal of Controlled Release, 2021, 329: 316-327.
104	LI Mengna, QIU Weiwang, WANG Qian, et al. Nitric oxide-releasing tryptophan-based poly(ester urea)s electrospun composite nanofiber mats with antibacterial and antibiofilm activities for infected wound healing[J]. ACS Applied Materials & Interfaces, 2022, 14(14): 15911-15926.

伪蛋白类型	合成方法
非功能性伪蛋白
AA-PEA	MP、IP、SP
AA-PEEA	SP
AA-PEU	IP、SP
功能性伪蛋白
AA-UPEA\\UPEEA AA-PEUU	IP、SP
AA-UPEA\\UPEEA AA-PEUU	SP
含有反应性侧链的伪蛋白	SP、ROP

伪蛋白类型	合成方法
非功能性伪蛋白
AA-PEA	MP、IP、SP
AA-PEEA	SP
AA-PEU	IP、SP
功能性伪蛋白
AA-UPEA\\UPEEA AA-PEUU	IP、SP
AA-UPEA\\UPEEA AA-PEUU	SP
含有反应性侧链的伪蛋白	SP、ROP

类型	聚合物	应用	参考文献	类型	聚合物	功能化	应用	参考文献
非功能性伪蛋白	Phe-PEA、Met-PEA	组织工程支架	[50]	功能性伪蛋白	Arg-UPEA	通过碳碳双键与普朗尼克丙烯酸（Pluronic-DA）前体光交联	水凝胶	[15]
	Phe-PEA	纳米颗粒	[65]		Arg-Leu-PEUU	自组装	纳米颗粒	[68]
	Phe-PEA	微球	[66]		Arg-Leu PEUU	马来酸官能化、引入交联烯烃与聚乙二醇二甲基丙烯酸酯原位交联	组织工程支架	[69]
	Arg-PEA	基因载体	[67]		PEA-Ser-OH	侧羟基丙烯化及光凝胶化	水凝胶	[63]
	Phe-PEU	软组织修复材料	[42]		PEA-Asp-COOH	偶联转化生长因子（TGF-b1）	组织工程	[64]
	Val-Phe-PEU	软组织修复材料	[55]		Lys-Arg-Phe-PEA	自组装	两亲性胶束	[70]

类型	聚合物	应用	参考文献	类型	聚合物	功能化	应用	参考文献
非功能性伪蛋白	Phe-PEA、Met-PEA	组织工程支架	[50]	功能性伪蛋白	Arg-UPEA	通过碳碳双键与普朗尼克丙烯酸（Pluronic-DA）前体光交联	水凝胶	[15]
	Phe-PEA	纳米颗粒	[65]		Arg-Leu-PEUU	自组装	纳米颗粒	[68]
	Phe-PEA	微球	[66]		Arg-Leu PEUU	马来酸官能化、引入交联烯烃与聚乙二醇二甲基丙烯酸酯原位交联	组织工程支架	[69]
	Arg-PEA	基因载体	[67]		PEA-Ser-OH	侧羟基丙烯化及光凝胶化	水凝胶	[63]
	Phe-PEU	软组织修复材料	[42]		PEA-Asp-COOH	偶联转化生长因子（TGF-b1）	组织工程	[64]
	Val-Phe-PEU	软组织修复材料	[55]		Lys-Arg-Phe-PEA	自组装	两亲性胶束	[70]

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