二氧化碳联合核桃壳提取液促进单针藻Monoraphidium sp. QLZ-3的生长和油脂积累

doi:10.16085/j.issn.1000-6613.2019-1220

摘要/Abstract

摘要：

以单针藻Monoraphidium sp. QLZ-3为对象，研究了CO₂对微藻在核桃壳提取液（walnut shell extracts，WSE）中生长及油脂积累的影响。结果显示，在12%的CO₂条件下，微藻在WSE中的生物量产率及油脂产率达到196.85mg/(L·d)和97.52mg/(L·d)，分别是对照组的1.33倍和1.57倍。WSE培养下，外源CO₂上调了微藻中核酮糖1,5-二磷酸羧化酶基因（ribulose 1,5-bisphosphate carboxylase/oxygenase，rbcL）的表达量，从而促进了CO₂的固定。此外，12% 的CO₂提高了微藻对WSE中多酚的利用，同时上调了乙酰辅酶A羧化酶（acetyl coenzyme A carboxylase，ACCase）和苹果酸酶（malic enzyme，ME）活性，下调了磷酸烯醇式丙酮酸羧化酶（phosphoenolpyruvate carboxylase，PEPC）活性。研究表明，CO₂可以提高WSE中微藻的生物量产率和油脂产率，降低培养成本，为核桃壳的资源化利用及微藻的工业化生产提供了新的理论基础。

关键词: 废物处理, 二氧化碳, 生物反应器, 生物柴油, 生物质

Abstract:

The effect of CO₂ on the cell growth and lipid accumulation of Monoraphidium sp. QLZ-3 in walnut shell extracts (WSE) was studied. Experimental results showed that the biomass and lipid productivities of QLZ-3 were 196.85mg/(L·d) and 97.52mg/(L·d) respectively under the presence of 12% CO₂, which were enhanced by 1.33-fold and 1.57-fold compared with the control group, respectively. The exogenous CO₂ upregulated the expression level of rbcL that was cultivated in WSE, and thus resulted in an increase in the CO₂ fixation. In addition, the 12% CO₂ increased the utilization of polyphenol, and increased the activities of the ACCase and ME but decreased those of PEPC of QLZ-3 in WSE. The results indicated that exogenous CO₂ could enhance the biomass and lipid productivity and reduce the cost of microalgae cultivation in WSE, which provided a new theoretical basis for the utilization of walnut shell and the industrial production of microalgae.

Key words: waste treatment, carbon dioxide, bioreactors, biodiesel, biomass

中图分类号:

邢海亮,董训赞,韩本勇,耿树香,宁德鲁,马婷,余旭亚. 二氧化碳联合核桃壳提取液促进单针藻Monoraphidium sp. QLZ-3的生长和油脂积累[J]. 化工进展, 2020, 39(4): 1575-1582.

Hailiang XING,Xunzan DONG,Benyong HAN,Shuxiang GENG,Delu NING,Ting MA,Xuya YU. Cell growth and lipid accumulation of Monoraphidium sp. QLZ-3 in walnut shell extracts with carbon dioxide[J]. Chemical Industry and Engineering Progress, 2020, 39(4): 1575-1582.

图/表 10

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引物	引物序列（5’-3’）	退火温度/℃
18S F	GGGAGTATGGTCGCAAGG	57
18S R	GACTATTTAGCAGGCTGAGGT
rbcL F	GTACCTGCCCAACCTCACCG	57
rbcL R	GCACCAGCATGGACACCACC

引物	引物序列（5’-3’）	退火温度/℃
18S F	GGGAGTATGGTCGCAAGG	57
18S R	GACTATTTAGCAGGCTGAGGT
rbcL F	GTACCTGCCCAACCTCACCG	57
rbcL R	GCACCAGCATGGACACCACC

脂肪酸	CO₂浓度
脂肪酸	对照组	4%	8%	12%	16%
C14∶0	0.28±0.0057	0.35±0.0099**	0.51±0.0021**	0.43±0.0014**	0.41±0.0057**
C16∶0	21.45±0.38	28.29±0.038**	30.55±0.049**	29.23±0.14**	29.30±0.049**
C16∶1	0.44±0.0085	0.14±0.0078**	0.17±0.0042**	0.20±0.0014**	0.28±0.013**
C18∶0	1.87±0.085	2.52±0.0078**	3.01±0.0042**	2.43±0.0035**	2.85±0.015**
C18∶1	24.06±0.089	29.05±0.012**	25.01±0.0057	24.24±0.0028	23.75±0.011
C18∶2	25.39±0.047	15.01±0.0049**	15.35±0.0085**	16.72±0.0071**	19.11±0.057**
C18∶3	22.76±0.12	19.18±0.0085**	20.33±0.0085*	21.76±0.0042	19.26±0.085**
C20∶0	0.16±0.014	0.22±0.0071**	0.24±0.0042**	0.21±0.0049**	0.24±0.0035**
C20∶1	1.44±0.022	1.89±0.0042**	1.47±0.0085	1.66±0.00071**	1.61±0.028**
C20∶3	0.026±0.0071	0.033±0.00071**	0.034±0.0035**	0.033±0.0014**	0.039±0.0028**
C22∶0	0.58±0.0028	0.9±0.028**	0.86±0.0078**	0.74±0.0049**	0.81±0.0085**
C22∶1	0.50±0.0092	0.75±0.0028**	0.69±0.0078**	0.66±0.0063**	0.63±0.0028**
C24∶0	0.35±0.0035	0.57±0.063**	0.59±0.011**	0.52±0.0063**	0.55±0.0028**
C24∶1	0.006±0.0014	0.028±0.0021**	0.029±0.00071**	0.008±0.0014**	0.026±0.0021**
SFA/%	24.69±0.47	32.85±0.048**	35.77±0.016**	33.57±0.023**	34.16±0.014**
MUFA/%	26.45±0.13	31.86±0.0078**	27.36±0.0028	26.77±0.013	26.29±0.021
PUFA/%	48.20±0.076	34.24±0.016**	35.71±0.011**	38.53±0.0099**	38.40±0.011**
DU/%	122.85±0.28	100.34±0.039**	98.77±0.020**	103.83±0.033**	103.10±0.044**
LCSF/%	4.81±0.069	6.80±0.076**	7.28±0.035**	6.51±0.0020**	6.91±00.024**
CFPP/℃	-16.32±0.0021	-16.3±0.0024	-16.25±0.0011	-16.27±0.0062	-16.26±0.0076

脂肪酸	CO₂浓度
脂肪酸	对照组	4%	8%	12%	16%
C14∶0	0.28±0.0057	0.35±0.0099**	0.51±0.0021**	0.43±0.0014**	0.41±0.0057**
C16∶0	21.45±0.38	28.29±0.038**	30.55±0.049**	29.23±0.14**	29.30±0.049**
C16∶1	0.44±0.0085	0.14±0.0078**	0.17±0.0042**	0.20±0.0014**	0.28±0.013**
C18∶0	1.87±0.085	2.52±0.0078**	3.01±0.0042**	2.43±0.0035**	2.85±0.015**
C18∶1	24.06±0.089	29.05±0.012**	25.01±0.0057	24.24±0.0028	23.75±0.011
C18∶2	25.39±0.047	15.01±0.0049**	15.35±0.0085**	16.72±0.0071**	19.11±0.057**
C18∶3	22.76±0.12	19.18±0.0085**	20.33±0.0085*	21.76±0.0042	19.26±0.085**
C20∶0	0.16±0.014	0.22±0.0071**	0.24±0.0042**	0.21±0.0049**	0.24±0.0035**
C20∶1	1.44±0.022	1.89±0.0042**	1.47±0.0085	1.66±0.00071**	1.61±0.028**
C20∶3	0.026±0.0071	0.033±0.00071**	0.034±0.0035**	0.033±0.0014**	0.039±0.0028**
C22∶0	0.58±0.0028	0.9±0.028**	0.86±0.0078**	0.74±0.0049**	0.81±0.0085**
C22∶1	0.50±0.0092	0.75±0.0028**	0.69±0.0078**	0.66±0.0063**	0.63±0.0028**
C24∶0	0.35±0.0035	0.57±0.063**	0.59±0.011**	0.52±0.0063**	0.55±0.0028**
C24∶1	0.006±0.0014	0.028±0.0021**	0.029±0.00071**	0.008±0.0014**	0.026±0.0021**
SFA/%	24.69±0.47	32.85±0.048**	35.77±0.016**	33.57±0.023**	34.16±0.014**
MUFA/%	26.45±0.13	31.86±0.0078**	27.36±0.0028	26.77±0.013	26.29±0.021
PUFA/%	48.20±0.076	34.24±0.016**	35.71±0.011**	38.53±0.0099**	38.40±0.011**
DU/%	122.85±0.28	100.34±0.039**	98.77±0.020**	103.83±0.033**	103.10±0.044**
LCSF/%	4.81±0.069	6.80±0.076**	7.28±0.035**	6.51±0.0020**	6.91±00.024**
CFPP/℃	-16.32±0.0021	-16.3±0.0024	-16.25±0.0011	-16.27±0.0062	-16.26±0.0076

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