一株耐盐菌的分离及其降解特性

doi:10.16085/j.issn.1000-6613.2017.03.037

化工进展 ›› 2017, Vol. 36 ›› Issue (03): 1047-1051.DOI: 10.16085/j.issn.1000-6613.2017.03.037

一株耐盐菌的分离及其降解特性

王丽娟, 钱子雯, 沈海波, 朱君, 吴彦霏, 陈孟君, 王利群

常州大学制药与生命科学学院, 江苏常州 213164

收稿日期:2016-06-08 修回日期:2016-09-01 出版日期:2017-03-05 发布日期:2017-03-05
通讯作者: 王利群,教授,硕士生导师,主要从事生物催化研究。
作者简介:王丽娟(1990-),女,硕士研究生。E-mail:1604383996@qq.com。

Separation and biodegradation characteristics of a halotolerant strain

WANG Lijuan, QIAN Ziwen, SHEN Haibo, ZHU Jun, WU Yanfei, CHEN Mengjun, WANG Liqun

School of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou 213164, Jiangsu, China

Received:2016-06-08 Revised:2016-09-01 Online:2017-03-05 Published:2017-03-05

摘要/Abstract

摘要：

煤化工废水是一种含盐量较高的难降解工业废水，为了提高其生化处理的效果，从某煤化工企业生产污水中分离得到一株耐盐菌CCZU-R6，经形态观察和16S rDNA序列分析，鉴定该菌株属于芽孢杆菌属（Bacillus sp.）。采用单因素实验方法考察了pH、温度、接种量、废水盐度等工艺条件对降解模拟含盐废水的影响，确定了菌株CCZU-R6处理模拟含盐废水的最适条件为pH 7.0、温度35℃，接种量500mg/L，能耐受的最高盐度约为11%。底物谱分析显示菌株CCZU-R6对苯酚、苯甲醇、甲醇等物质具有显著的降解效果。在最适降解条件下，菌株CCZU-R6处理实际煤化工含盐废水24h，COD去除率达到93%。表明该菌株对含盐煤化工废水有较强的降解能力，具有工业应用前景。

关键词: 耐盐菌, 分离, 降解, 含盐废水

Abstract:

Coal chemical industry wastewater is a kind of refractory industry wastewater with high salt content. In order to improve biodegradation efficiency of coal chemical industry wastewater,a halotolerant bacteria CCZU-R6 was isolated from the sewage of a coal chemical enterprise. It was identified as Bacillus sp. by its morphological properties and 16S rDNA sequence analysis. Using the method of single factor experiment,the effects of degradation conditions were studied. The optimum treatment conditions for simulated saline wastewater were pH 7.0,temperature 35℃,inoculum concentration 500mg/L,and the maximum salinity tolerance was about 11%. Substrate spectral analysis showed that CCZU-R6 could degrade phenol,benzyl alcohol,and methanol. The COD removal rate was up to 93% when the coal chemical industry saline wastewater was treated by CCZU-R6 for 24 hours under the optimal conditions. The results showed that CCZU-R6 could effectively degrade saline wastewater in coal chemical industry,with good commercial application potential.

Key words: halotolerant strain, separation, degradation, saline wastewater

中图分类号:

X703
X172

王丽娟, 钱子雯, 沈海波, 朱君, 吴彦霏, 陈孟君, 王利群. 一株耐盐菌的分离及其降解特性[J]. 化工进展, 2017, 36(03): 1047-1051.

WANG Lijuan, QIAN Ziwen, SHEN Haibo, ZHU Jun, WU Yanfei, CHEN Mengjun, WANG Liqun. Separation and biodegradation characteristics of a halotolerant strain[J]. Chemical Industry and Engineering Progress, 2017, 36(03): 1047-1051.

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一株耐盐菌的分离及其降解特性

Separation and biodegradation characteristics of a halotolerant strain

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