受激辐射损耗超分辨荧光成像探针研究进展

doi:10.16085/j.issn.1000-6613.2018-1145

化工进展 ›› 2019, Vol. 38 ›› Issue (02): 726-739.DOI: 10.16085/j.issn.1000-6613.2018-1145

受激辐射损耗超分辨荧光成像探针研究进展

刘毋凡(),陈楚芳,潘文慧,熊佳,屈军乐,杨志刚()

深圳大学光电工程学院，光电子器件与系统教育部/广东省重点实验室，广东深圳 518060

收稿日期:2018-05-30 修回日期:2018-10-01 出版日期:2019-02-05 发布日期:2019-02-05
通讯作者: 杨志刚
作者简介:<named-content content-type="corresp-name">刘毋凡</named-content>（1993—），男，硕士研究生，研究方向为受激辐射损耗超分辨成像及荧光寿命成像。E-mail：<email>749969710@qq.com</email>。|杨志刚，副教授，硕士生导师，研究方向为生物光子学影像材料、超分辨成像及荧光寿命成像。E-mail：<email>zhgyang@szu.edu.cn</email>。
基金资助:
深圳市基础科学研究自由探索项目(JCYJ20170818100931714);国家自然科学基金(61875131/21406125)

Research progress on stimulated emission depletion using fluorescent probes

Wufan LIU(),Chufang CHEN,Wenhui PAN,Jia XIONG,Junle QU,Zhigang YANG()

Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, Guangdong, China

Received:2018-05-30 Revised:2018-10-01 Online:2019-02-05 Published:2019-02-05
Contact: Zhigang YANG

摘要/Abstract

摘要：

受激辐射损耗超分辨成像可突破光学衍射极限的限制，获得纳米尺寸结构的超精细图像，荧光探针发挥了重要作用。本文主要介绍了受激辐射损耗超分辨显微成像的相关概念，包括基础光学概念、受激辐射损耗超分辨成像原理、成像系统，总结了受激辐射损耗超分辨成像荧光探针及其应用等研究进展，相信本工作能帮助化学、化工领域相关工作者了解受激辐射超分辨成像研究及其生物成像应用，尤其是可以用于该成像技术的荧光探针的相关知识，为设计、制备有效的受激辐射超分辨荧光探针提供设计思路。本文为荧光探针在生物医学光学领域的应用提出了新的需求与机遇，为化学、化工领域相关研究方向与光学成像领域的深度交叉与融合提供了新的发展契机。

关键词: 成像分辨率, 超分辨成像, 受激辐射, 受激辐射损耗超分辨成像, 超分辨成像探针

Abstract:

Stimulated emission depletion super-resolution microscopy(STED) can overcome the limit of optical diffraction to achieve the ultra-fine imaging of nanoscale of targets. The fluorescent probes play great roles in this technique. In this paper, the basic concept of STED microscopy was introduced, including the basic optical concept, principle of STED microscopy and imaging system, and STED fluorescent probes and the biological applications were summarized. This paper would be beneficial to the researchers from the fields of chemistry and chemical engineering in understanding of fundamental theory of superresolution imaging microscopy, which is helpful for the design of effective fluorescent probes in STED superresolution imaging. This review also provided the new requirement for fluorescent probes in biomedical imaging area, and the new opportunity for the joint disciplines in chemistry and chemical engineering with optical imaging.

Key words: imaging resolution, superresolution imaging, stimulated emission, stimulated emission depletion superresolution imaging microscopy, superresolution imaging probes

中图分类号:

刘毋凡, 陈楚芳, 潘文慧, 熊佳, 屈军乐, 杨志刚. 受激辐射损耗超分辨荧光成像探针研究进展[J]. 化工进展, 2019, 38(02): 726-739.

Wufan LIU, Chufang CHEN, Wenhui PAN, Jia XIONG, Junle QU, Zhigang YANG. Research progress on stimulated emission depletion using fluorescent probes[J]. Chemical Industry and Engineering Progress, 2019, 38(02): 726-739.

图/表 13

图1 瑞利判据区分两个艾里斑的示意图

图2 荧光分子的雅布伦斯基能级图

图3 共聚焦激光扫描显微镜（CLSM）简化图

图4 苝酰二亚胺化合物（1，2）的分子结构[23]

图5 荧光分子探针（3～6）的化学结构

图6 荧光探针（7a～7d）的化学结构式[25]

图7 硅杂罗丹明的生物正交标记高尔基体的实验过程[26]

图8 荧光探针（10～12）的化学结构

图9 化合物13～18的化学结构[35]

图10 聚集发光有机小分子探针结构及自组装纳米结构示意图[42]

图11 CdSe量子点用于STED超分辨成像中激发、损耗波长的选择[43]

图12 上转换纳米量子点的受激辐射机理及在不同损耗光能量激发下的成像分辨率[44]

图13 钙钛矿量子点的电镜表征与STED超分辨成像[45]

参考文献 45

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受激辐射损耗超分辨荧光成像探针研究进展

Research progress on stimulated emission depletion using fluorescent probes

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