小球藻在内光源气升式反应器中的培养工艺优化

doi:10.16085/j.issn.1000-6613.2017-0859

化工进展 ›› 2018, Vol. 37 ›› Issue (02): 525-532.DOI: 10.16085/j.issn.1000-6613.2017-0859

小球藻在内光源气升式反应器中的培养工艺优化

张芬芬^1,3, 马晓建^1,2, 方书起^1,2, 白净^1,2, 常春^1,2

1 郑州大学化工与能源学院, 河南郑州 450001;
2 生物质炼制技术与装备河南省工程实验室, 河南郑州 450001;
3 中航锂电(洛阳)有限公司, 河南洛阳 471000

收稿日期:2017-05-10 修回日期:2017-07-07 出版日期:2018-02-05 发布日期:2018-02-05
通讯作者: 常春,博士,副教授,研究方向为生物质能源化工。
作者简介:张芬芬(1991-),女,硕士研究生。
基金资助:
河南省自然科学基金（162300410247）及河南省基础与前沿技术项目（162300410007）。

Optimization of culture technology of Chlorella vulgaris in airlift reactors with internal light source

ZHANG Fenfen^1,3, MA Xiaojian^1,2, FANG Shuqi^1,2, BAI Jing^1,2, CHANG Chun^1,2

1 School of Chemical Engineering and Energy, Zhengzhou University, Zhengzhou 450001, Henan, China;
2 Engineering Laboratory of Henan Province for Biorefinery Technology and Equipment, Zhengzhou 450001, Henan, China;
3 China Aviation Lithium Battery Co., Ltd., Luoyang 471000, Henan, China

Received:2017-05-10 Revised:2017-07-07 Online:2018-02-05 Published:2018-02-05

摘要/Abstract

摘要： 为探索小球藻在内光源气升式光生物反应器中的培养工艺，本文采用自行设计的50L内光源气升式反应器为实验装置，以藻细胞密度为考察指标，采用单因素法分别考察了内置光源波长、光源强度、光暗周期以及二氧化碳供应量对小球藻生长的影响。在此基础上，利用响应面设计法对工艺条件进行了优化，优化结果为：反应器内置光源为红蓝光，光照强度9615lux，光暗周期17.5h：6.5h，二氧化碳通气量为30L/h。在该优化条件下，进行3次验证实验，经15天培养，小球藻的收获藻细胞密度均值为5.48×10⁷ cells/mL，与预测值5.5×10⁷ cells/mL相近。收获小球藻干重为1.21g/L，相较优化前提高了157%。该结果为内光源气升式反应器在微藻培养的应用提供了重要的参考。

关键词: 小球藻, 内光源气升式反应器, 响应面法, 藻细胞密度

Abstract: To explore the optimal cultivation conditions of Chlorella vulagris in the airlift photo-bioreactor, a custom-designed 50 L airlift reactor with an internal light source was developed, and the influence of light wavelength, intensity of light source, light and dark cycles and supply of carbon dioxide on the growth of Chlorella vulagris were investigated in this study. Single factor experiments were firstly conducted with algae cell density as the investigation index. Based on that, the response surface methodology was further adapted to optimize the process conditions. The optimal conditions were as follows:red-blue lights as light source, 9615 lux of light intensity, 17.5h:6.5h of light-dark cycles and 30L/h of carbon dioxide ventilation. Under the optimal conditions, three verification experiments were performed. After 15 days cultivation, the harvested cell density of Chlorella vulagris was 5.48×10⁷ cells/mL, which was close to the results predicted, and the dry weight was 1.21 g/L, increased by 157%. These results can provide an important reference for the application of internal light source airlift reactor in microalgae culture.

Key words: Chlorella vulagris, air-lift reactor with internal light source, response surface methodology, algae cell density

中图分类号:

张芬芬, 马晓建, 方书起, 白净, 常春. 小球藻在内光源气升式反应器中的培养工艺优化[J]. 化工进展, 2018, 37(02): 525-532.

ZHANG Fenfen, MA Xiaojian, FANG Shuqi, BAI Jing, CHANG Chun. Optimization of culture technology of Chlorella vulgaris in airlift reactors with internal light source[J]. Chemical Industry and Engineering Progress, 2018, 37(02): 525-532.

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小球藻在内光源气升式反应器中的培养工艺优化

Optimization of culture technology of Chlorella vulgaris in airlift reactors with internal light source

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