自污染染料中筛选的菌对颜料紫1的脱色

doi:10.16085/j.issn.1000-6613.2015.04.030

化工进展 ›› 2015, Vol. 34 ›› Issue (04): 1074-1079,1097.DOI: 10.16085/j.issn.1000-6613.2015.04.030

自污染染料中筛选的菌对颜料紫1的脱色

胡翠英, 沈玉婷, 李良智, 钱玮, 郭伟强

苏州科技学院化学生物与材料工程学院, 江苏苏州 215009;
江苏省环境功能材料重点实验室, 江苏苏州 215009

收稿日期:2014-09-11 修回日期:2014-10-13 出版日期:2015-04-05 发布日期:2015-04-05
通讯作者: 李良智,博士,副教授,研究方向为生物化工.E-mail liliangzhi01@163.com.
作者简介:胡翠英(1978—),女,硕士,实验师.E-mai 13151170848@163.com.
基金资助:
江苏高校优势学科建设工程(PAPD)及苏州市应用基础研究(SYN201321)项目.

Decoloration of pigment violent 1 by the strain isolated from stained dye

HU Cuiying, SHEN Yuting, LI Liangzhi, QIAN Wei, GUO Weiqiang

College of Chemistry, Biology and Materials Engineering, Suzhou University of Science and Technology, Suzhou 215009, Jiangsu, China;
Jiangsu Key Laboratory for Environment Functional Materials, Suzhou 215009, Jiangsu, China

Received:2014-09-11 Revised:2014-10-13 Online:2015-04-05 Published:2015-04-05

摘要/Abstract

摘要： 为研究颜料紫1的脱色问题,从一盒长满菌苔的广告浓缩染料(主要显色原料颜料紫1)中筛选菌株,通过摇瓶培养、以及平板分离等获得了单一纯种菌株、并进一步对该菌株进行了18S rDNA测序分析,从而鉴定出该菌为杂色曲霉(Aspergillus versicolor).在此基础上,系统地研究了该菌株对颜料紫1的脱色条件.综合单因素实验和响应面法优化实验的结果表明:该杂色曲霉菌对于颜料紫1脱色的最佳条件为碳源葡萄糖 30g/L,颜料紫1起始含量为250 mg/L,NaNO₃含量为0.3g/L,pH 值5.5,这时可获得最高的颜料紫1脱色率为84.64%.最后,通过傅里叶变换红外光谱仪(FTIR)分析,揭示了该杂色曲霉菌对颜料紫1的脱色机制主要为吸附脱色.

关键词: 染料脱色, 杂色曲霉菌, 颜料紫1, 响应面法, 脱色机制

Abstract: To investigate the decoloration problem of pigment violent 1,a strain was isolated from advertising dye,whose main composition was pigment violent 1. A single and pure strain was first obtained by flask shake and dish culture methods. 18S rDNA gene sequence analysis identified that the strain was Aspergillus versicolor. Then the decoloration conditions of pigment violent 1 were studied systematically by this novel strain. The optimal condition was found by mono factor experiment and response surface experiment as the glucose 30g/L,the original concentration of pigment violent 1 250 mg/L,the concentration of NaNO₃ 0.3g/L,and the pH 5.5. Under the optimal decoloration condition,the decoloration rate of pigment violent 1 reached 84.64% by the strain Aspergillus versicolor. The discoloration mechanism of pigment violent 1 was analyzed and characterized by FTIR,and the results indicated that the main mechanism of the decoloration was bio-adsorption.

Key words: dye decolorization, Aspergillus versicolor, pigment violent 1, response surface methodology, the discolored mechanism

中图分类号:

S216.2

胡翠英, 沈玉婷, 李良智, 钱玮, 郭伟强. 自污染染料中筛选的菌对颜料紫1的脱色[J]. 化工进展, 2015, 34(04): 1074-1079,1097.

HU Cuiying, SHEN Yuting, LI Liangzhi, QIAN Wei, GUO Weiqiang. Decoloration of pigment violent 1 by the strain isolated from stained dye[J]. Chemical Industry and Engineering Progree, 2015, 34(04): 1074-1079,1097.

参考文献

[1] Sharma P ,Kaur H ,Sharma M ,et al. A review on applicability of naturally available adsorbents for the removal of hazardous dyes from aqueous waste[J]. Environ. Monit. Assess.,2011,183:151-195.
[2] Chowdhury S,Chakraborty S,Saha P D. Response surface optimization of a dynamic dye adsorption process:A case study of crystal violet adsorption onto NaOH-modified rice husk[J]. Environ. Sci. Pollut. Res.,2013,20(3):1698-1705.
[3] Sun D,Zhang X ,Wu Y ,et al. Kinetic mechanism of competitive adsorption of disperse dye and anionic dye on fly ash[J]. Int. J. Environ. Sci. Technol.,2013,10(4):799-808.
[4] Yuan Y L,Wen Y Z,Li X Y,et al. Treatment of wastewater from dye manufacturing industry by coagulation[J]. J. Zhejiang Univ. Sci. A,2006,7(S2):340-344.
[5] Parsa J B,Chianeh F N. Evaluation of electro-coagulation method for decolorization and degradation of organic dyes in aqueous solutions[J]. Korean J. Chem. Eng.,2012,29(11):1585-1590.
[6] Bhowon M G,Laulloo S J,Wah H L K,et al. Chemistry Education in the ICT Age[M]. America:Springer Netherlands,2009:225-234.
[7] Vinodgopal K,Peller J. Hydroxyl radical-mediated advanced oxidation processes for textile dyes:A comparison of the radiolytic and sonolytic degradation of the monoazo dye Acid Orange 7[J]. Res. Chem. Intermed.,2003,29(3):307-316.
[8] 刘新颖,朱启忠,赵春媛,等. 双孢菇漆酶酶学性质及染料脱色初探[J]. 食用菌,2010(14):182-183.
[9] Kapdan I ,Kargi F,Mcmullan G,et al. Comparison of white-rot fungi cultures for decolorization of textile dyestuffs[J]. Bioprocess. Eng.,2000,22(4):347-351.
[10] Prasad A S A ,Rao K V B. Aerobic biodegradation of Azo dye by Bacillus cohnii MTCC 3616;an obligately alkaliphilic bacterium and toxicity evaluation of metabolites by different bioassay systems[J]. Appl. Microbiol. Biotechnol.,2013,97(16):7469-7481.
[11] Vijayalakshmidevi S R,Muthukumar K. Biodegradation of malachite green by Ochrobactrum sp.[J]. World J. Microbiol. Biotechnol.,2014,30(2):429-437.
[12] 李慧,曲媛媛,时胜男,等. 一株高效广谱染料降解细菌的分离鉴定及其脱色特性初探[J]. 微生物学通报,2011,38(4):523-530.
[13] Bhawana P,Fulekar M H. Bioremediation of dyestuff compounds using indigenous microorganism in a bioreactor[J]. Procedia APCBEE,2012,1(1):27-33.
[14] Dellai A,Dridi D,Lemorvan V,et al. Decolorization does not always mean detoxification:Case study of a newly isolated Pseudomonas peli for decolorization of textile wastewater[J]. Environ. Sci. Pollut. Res.,2013,20(8):5790-5796.
[15] 张朝晖,夏黎明,林建平,等. 黄孢原毛平革菌所产木素过氧化物酶系对染料降解的研究[J]. 环境污染与防治,2002,24(2):71-76.
[16] 李慧蓉,陈武,陈和谦. 黄孢原毛平革菌对三苯甲烷染料的生物脱色降解[J]. 上海环境科学,2003,22(11):738-742.

[17] Belofsky G N,Renner M K,Fenical W,et al. New cytotoxic sesquiterpenoid nitrobenzoyl esters from a marine isolate of the fungus Aspergillus versicolor[J]. Tetrahedron,1998,54(9):1715-1724.

[18] Lee Y M,Li H Y,Hong J,et al. Bioactive metabolites from the sponge-derived fungus Aspergillus versicolor[J]. Arch. Pharm. Res.,2010,33(2):231-235.

[19] Chung D H,Abouzied M M,Pestka J J. Immunochemical assay applied to mycotoxin biosynthesis:ELISA comparison of sterigmatocystin production by Aspergillus versicolor and Aspergillus nidulans[J]. Mycopathologia,1989,107(2-3):93-100.

[20] Carvalho M R,Barbosa L C A,Queiróz J H,et al. Novel lactones from Aspergillus versicolor; production of sterigmatocystin by Aspergillus versicolor QM 432 used in fungus resistance tests for United States military specifications[J]. Tetrahedron Lett.,2001,42(5):809-811.

[21] Bairagia H,Khan M M R,Ray L,et al. Adsorption profile of lead on Aspergillus versicolor:A mechanistic probing[J]. J. Hazard. Mater.,2011,186(1):756-764.

[22] Tastan B E,Ertuğrul S,Dönmez G. Effective bioremoval of reactive dye and heavy metals by Aspergillus versicolor[J]. Bioresour. Technol.,2010,101(3):870-876.

[23] Zhao J H,Wang X Q,Zeng J,et al. Biodegradation of poly(butylene succinate-co-butylene adipate) by Aspergillus versicolor[J]. Polym. Degrad. and Stabil.,2005,90(1):173-179.

[24] Krupiński M,Szewczyk R,Dlugoński J. Detoxification and elimination of xenoestrogen nonylphenol by the filamentous fungus Aspergillus versicolor[J]. Int. Biodeterior. and Biodegrad.,2013,82:59-66.

[25] Gül Ü D,Dönmez G. Effects of dodecyl trimethyl ammonium bromide surfactant on decolorization remazol blue by a living Aspergillus versicolor strain[J]. J. Surfactants Deterg.,2012,15(6):797-803.

[26] Asgher M. Biosorption of reactive dyes:A review[J]. Water Air Soil Pollut.,2012,223(5):2417-2435.

自污染染料中筛选的菌对颜料紫1的脱色

Decoloration of pigment violent 1 by the strain isolated from stained dye

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