高效功能菌群RR的筛选及其群落分析

doi:10.16085/j.issn.1000-6613.2015.07.027

化工进展 ›› 2015, Vol. 34 ›› Issue (07): 1973-1978,2022.DOI: 10.16085/j.issn.1000-6613.2015.07.027

高效功能菌群RR的筛选及其群落分析

朱玲玉, 谢学辉, 刘娜, 杨芳, 俞承志, 柳建设

东华大学环境科学与工程学院, 上海 201620

收稿日期:2014-12-01 修回日期:2014-12-10 出版日期:2015-07-05 发布日期:2015-07-05
通讯作者: 柳建设,博士,教授,博士生导师,主要研究方向为环境微生物。E-mail:liujianshe@dhu.edu.cn。
作者简介:朱玲玉(1989—),女,硕士,主要从事环境微生物技术方向研究。E-mail:skylar_dream@163.com。
基金资助:
国家自然科学基金面上项目(21377023)、教育部博士点新教师基金(20120075120014)、上海市自然科学基金青年项目(12ZR1440400)、上海市重点学科建设项目(B604)及中央高校基本科研业务费专项资金(2232015D3-22)。

Isolation of high performance bacterial consortium RR and its bacterial community composition

ZHU Lingyu, XIE Xuehui, LIU Na, YANG Fang, YU Chengzhi, LIU Jianshe

College of Environmental Science and Engineering, Donghua University, Shanghai 201620, China

Received:2014-12-01 Revised:2014-12-10 Online:2015-07-05 Published:2015-07-05

摘要/Abstract

摘要： 利用梯度浓度压力驯化法, 从厌氧反应器中筛选出对直接红28有具有良好脱色能力的混合菌群RR。在染料初始浓度为200mg/L, pH=7.0, 温度为35℃条件下, 经48h静止培养, 染料脱色率可达96.16%。进一步对其培养条件如pH值、温度、盐度、初始染料浓度等进行了脱色研究, 结果表明, 在pH=7、温度为45℃, 盐度为2mmol/L的情况下, 功能菌群脱色效果达到最佳。为了进一步适应工程无机条件, 筛选出以染料作为唯一氮源、碳源以及能量的功能菌群, 遂将培养基中葡萄糖去掉, 同样利用梯度浓度压力驯化法, 筛选出混合菌群RM, 并对其脱色性能及群落结构进行分析。混合菌群RM在染料初始浓度为50mg/L、温度为35℃、pH=7.0条件下, 48h后其脱色效率为20.05%。利用变性梯度凝胶电泳(DGGE)法, 对群落进行分析, 混合菌群RR主要为伯克霍尔德氏菌属(Burkholderia sp.)、链球菌属(Streptococcus)和克雷伯氏菌属(Klebsiella sp.), 菌群RM主要为伯克霍尔德氏菌属(Burkholderia sp.), 可见伯克霍尔德氏菌属菌株(Burkholderia sp.)可以适应工程无机环境, 并对直接红28存在一定的降解能力。

关键词: 染料脱色, 混合菌群, 群落分析, 变性梯度凝胶电泳

Abstract: A bacterial consortium capable of decoloring Direct Red 28 effectively was screened from a well-running textile printing wastewater biological treatment system, through the method of domestication with gradient concentrations. The bacterial consortium RR had the ability to decolorize Direct Red 28 (at 35℃, pH 7.0, initial dye concentration 200mg/L ) with the decolorization rate being 96.16% in 48h. The optimum pH, temperature, and NaCl concentration for the degradation of Direct Red 28 are 7.0, 45℃, and 2mmol/L, respectively. To screen the bacterial consortium RM which fed on Direct Red 28 was to ensure the bacterial consortium to adapt the inorganic conditions. The bacterial consortium RM degrading Direct Red 28 showed the decolorization efficiency of 20.05% (at 35℃, pH 7.0, initial dye concentration 50mg/L ).Through denaturing gradient gel electrophoresis(DGGE) to analyze the composition of microbial communities of the flora RR and RM. DGGE result showed that the dominant groups in the flora RR were Burkholderia bacteria, Streptococcus bacteria and Klebsiella bacteria and the dominant groups in the flora RM was Burkholderia bacteria. The results showed that the Burkholderia bacteria could survive in the inorganic environment and had the ability to degrade the Direct Red 28.

Key words: biological decoloring, bacterial consortium, composition of microbial communities, DGGE

中图分类号:

Q936

朱玲玉, 谢学辉, 刘娜, 杨芳, 俞承志, 柳建设. 高效功能菌群RR的筛选及其群落分析[J]. 化工进展, 2015, 34(07): 1973-1978,2022.

ZHU Lingyu, XIE Xuehui, LIU Na, YANG Fang, YU Chengzhi, LIU Jianshe. Isolation of high performance bacterial consortium RR and its bacterial community composition[J]. Chemical Industry and Engineering Progree, 2015, 34(07): 1973-1978,2022.

参考文献

[1] 何兴兵, 林永慧, 韩国民, 等. 开放条件下烟管菌XX-2对孔雀石绿染料的高效降解[J]. 微生物学通报, 2013(7):1163-1174.
[2] 张培培, 任随周, 许玫英, 等. 微生物对三苯基甲烷类染料脱色的研究进展[J]. 微生物学通报, 2009(9):1410-1417.
[3] Khan R, Bhawana P, Fulekar M H, et al. Microbial decolorization and degradation of synthetic dyes:A review[J]. Reviews in Environmental Science and Bio-Technology, 2013, 12(1):75-97.
[4] Saratale R G, Saratale G D, Chang J S, et al. Bacterial decolorization and degradation of azo dyes: A review[J]. Journal of the Taiwan Institute of Chemical Engineers, 2011, 42(1):138-157.
[5] Patel Y, Mehta C, Gupte A, et al. Assessment of biological decolorization and degradation of sulfonated di-azo dye Acid Maroon V by isolated bacterial consortium EDPA[J]. International Biodeterioration & Biodegradation, 2012, 75:187-193.
[6] Arunarani A, Chandran P, Ranganathan B V, et al. Bioremoval of Basic Violet 3 and Acid Blue 93 by Pseudomonas putida and its adsorption isotherms and kinetics[J]. Colloids and Surfaces B:Biointerfaces, 2013, 102:379-384.
[7] Liu J S, Xie X H, Xiao S M, et al. Isolation of Leptospirillum ferriphilum by single-layered solid medium[J]. Journal of Central South University of Technology, 2007, 14(4):467-473.
[8] Prasad S S, Aikat K. Study of bio-degradation and bio-decolourization of azo dye by Enterobacter sp. SXCR[J]. Environmental Technology, 2014, 35(8):956-965.
[9] Anjaneya O, Souche S Y, Santoshkumar M, et al. Decolorization of sulfonated azo dye Metanil Yellow by newly isolated bacterial strains: Bacillus sp. strain AK1 and Lysinibacillus sp. strain AK2[J]. Journal of Hazardous Materials, 2011, 190(1-3):351-358.
[10] Chen K C, Wu J Y, Liou D J, et al. Decolorization of the textile dyes by newly isolated bacterial strains[J]. Journal of Biotechnology, 2003, 101(1):57-68.
[11] Kılıç N K, Nielsen J L, Yüce M, et al. Characterization of a simple bacterial consortium for effective treatment of wastewaters with reactive dyes and Cr(Ⅵ) [J]. Chemosphere, 2007, 67(4):826-831.
[12] Kumar K, Dastidar M, Sreekrishnan T. Effect of process parameters on aerobic decolourization of reactive azo dye using mixed culture[J]. World Academy of Science, Engineering and Technology, 2009, 58:962-965.
[13] Jadhav U U, Dawkar V V, Ghodake G S, et al. Biodegradation of Direct Red 5B, a textile dye by newly isolated Comamonas sp. UVS[J]. Journal of Hazardous Materials, 2008, 158(2):507-516.
[14] Pearce C, Lloyd J, Guthrie J. The removal of colour from textile wastewater using whole bacterial cells: A review[J]. Dyes and Pigments, 2003, 58(3):179-196.
[15] He Y, Gao J F, Feng F Q, et al. The comparative study on the rapid decolorization of azo, anthraquinone and triphenylmethane dyes by zero-valent iron[J]. Chemical Engineering Journal, 2012, 179(0):8-18.
[16] 王银善, 庞学军, 方慈祺, 等. 共生细菌SB1降解聚乙烯醇的研究Ⅰ. 共生细菌SB1的分离及某些性质[J]. 环境科学学报, 1991, 11(2):236-241.
[17] Laszlo J A. Regeneration of azo-dye-saturated cellulosic anion exchange resin by Burkholderia cepacia anaerobic dye reduction[J]. Environmental Science & Technology, 2000, 34(1):167-172.
[18] Arora P K, Srivastava A, Singh V P. Novel degradation pathway of 4-chloro-2-aminophenol via 4-chlorocatechol in Burkholderia sp. RKJ 800[J]. Environmental Science and Pollution Research, 2014, 21(3):2298-2304.
[19] Zhou F, Cheng Y, Gan L, et al. Burkholderia vietnamiensis C09V as the functional biomaterial used to remove crystal violet and Cu(II) [J]. Ecotoxicology and Environmental Safety, 2014, 105: 1-6.
[20] Cui D, Li G, Zhao M, et al. Decolourization of azo dyes by a newly isolated Klebsiella sp. strain Y3, and effects of various factors on biodegradation[J]. Biotechnology & Biotechnological Equipment, 2014, 28(3):478-486.

高效功能菌群RR的筛选及其群落分析

Isolation of high performance bacterial consortium RR and its bacterial community composition

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 7

编辑推荐

Metrics

本文评价

[1]	杨景瑞, 王莹, 陈虎, 吕永康. 氧浓度对调节NO _x 氧化度协同CABR法脱硝性能及菌群结构的影响[J]. 化工进展, 2022, 41(11): 6139-6148.
[2]	汪润民, 张晓东, 徐成华, 于丹丹, 余冉. 高效重质石油降解菌群构建及降解性能评价[J]. 化工进展, 2022, 41(10): 5653-5660.
[3]	李泽兵,潘昕,刘顺亮,毛静岩,孙占学,张卫民. 复合嗜酸亚铁氧化柱的长期运行特征[J]. 化工进展, 2019, 38(11): 5173-5180.
[4]	俞承志, 谢学辉, 郑秀林, 徐乐铱, 李然, 柳建设. 活性黑5的生物脱色、复色现象及机理[J]. 化工进展, 2016, 35(09): 2987-2996.
[5]	胡翠英, 沈玉婷, 李良智, 钱玮, 郭伟强. 自污染染料中筛选的菌对颜料紫1的脱色[J]. 化工进展, 2015, 34(04): 1074-1079,1097.
[6]	杨倩1，蒋阳月1，王小军1，陈英文1，沈树宝1，石利利2，刘济宁2. 不同区域污水处理厂活性污泥中微生物菌落结构分析[J]. 化工进展, 2014, 33(12): 3329-3336.
[7]	刘英杰1，贾晓强1，2，3，闻建平1，2，3，班睿1. 混合菌群合成聚羟基脂肪酸酯研究进展[J]. 化工进展, 2014, 33(10): 2729-2734.