蜡状芽孢杆菌菌株SMC的间甲酚降解特性及动力学

doi:10.16085/j.issn.1000-6613.2015.05.044

化工进展 ›› 2015, Vol. 34 ›› Issue (05): 1453-1458.DOI: 10.16085/j.issn.1000-6613.2015.05.044

蜡状芽孢杆菌菌株SMC的间甲酚降解特性及动力学

于晨阳, 毛缜

中国矿业大学(徐州)环境与测绘学院, 江苏徐州 221116

收稿日期:2014-09-29 修回日期:2014-11-05 出版日期:2015-05-05 发布日期:2015-05-05
通讯作者: 毛缜,副教授,硕士生导师.E-mailbingyuer0120@163.com.
作者简介:于晨阳(1991—),女,硕士研究生.

Characteristics and kinetics study of m-cresol biodegradation by Bacillus cereus strain SMC

YU Chenyang, MAO Zhen

School of Environmental Science and Spatial Informatics, China University of Mining and Technology, Xuzhou 221116, Jiangsu, China

Received:2014-09-29 Revised:2014-11-05 Online:2015-05-05 Published:2015-05-05

摘要/Abstract

摘要： 针对高浓度间甲酚废水难降解的问题,通过筛选获得一株高效降解间甲酚的菌株,并进一步探究其对间甲酚的降解特性和降解动力学.本文从某焦化厂活性污泥中经过驯化、筛选分离得到一株以间甲酚为唯一碳源的高效降解菌株,对其进行16S rDNA分子鉴定;通过测定培养液中间甲酚的剩余浓度,考察在菌株对间甲酚的最佳降解条件;并采用Haldane模型来模拟菌株降解间甲酚的动力学行为.将分离得到的菌株命名为SMC,初步鉴定为Bacillus cereus;在pH值为7.5、温度为30℃、摇床转速为150r/min的最佳培养条件下,菌株SMC在48h内对浓度1600mg/L的间甲酚降解率达97.81%,对间甲酚最大耐受能力为1900mg/L;经过Haldane模型拟合后的动力学参数为μ_max=0.01252h^-1,K_S=34.58mg/L,K_i=479.5mg/L(R²=0.932).菌株SMC对间甲酚有较强的降解能力,在高效处理含酚废水方面具有一定的应用前景.

关键词: 间甲酚, 生物降解, 动力学模型, 废水处理

Abstract: A bacterial strain SMC was isolated from the aerobic sludge of coking wastewater to resolve the problem of difficulty in treating effluent with high concentration of m-cresol. The characteristics and kinetics of degradation of m-cresol by the strain was investigated with m-cresol as sole source of carbon in the cultural medium. After isolation and screening,16S rDNA gene was analyzed to identify the taxonomic position of the strain. Its degradation capability under optimal growth conditions was studied by testing the concentration of m-cresol in residue medium. Haldane type substrate inhibit model was used to simulate cell growth on m-cresol. The isolated strain named as SMC belonged to Bacillus cereus. Under the optimal conditions of pH 7.5、30℃,150r/min of shaking rate,m-cresol with 1600mg/L initial concentration would be degraded 97.81% in 48h and the highest tolerance to m-cresol was 1900mg/L. The kinetic parameters were:μ_max=0.01252h^-1,K_S=34.58mg/L,K_i=479.5mg/L(R² = 0.932). SMC could degrade m-cresol effectively,with good application potential for wastewater containing high concentration of m-cresol.

Key words: m-cresol, biodegradation, kinetic modeling, waste water treatment

中图分类号:

X172
TQ085

于晨阳, 毛缜. 蜡状芽孢杆菌菌株SMC的间甲酚降解特性及动力学[J]. 化工进展, 2015, 34(05): 1453-1458.

YU Chenyang, MAO Zhen. Characteristics and kinetics study of m-cresol biodegradation by Bacillus cereus strain SMC[J]. Chemical Industry and Engineering Progree, 2015, 34(05): 1453-1458.

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蜡状芽孢杆菌菌株SMC的间甲酚降解特性及动力学

Characteristics and kinetics study of m-cresol biodegradation by Bacillus cereus strain SMC

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