In order to explore the influence of organic load fluctuation frequency on aerobic granular sludge (AGS) under low-intensity influent conditions, three parallel cylindrical sequencing batch reactors(SBR), R1, R2 and R3, were operated by constant organic loading rate (OLR) and two alternating OLR modes respectively, fed with synthetic water. Their high and low OLR were 0.67gCOD/(L∙d)/0.67gCOD/(L∙d), 0.74gCOD/(L∙d)/0.56gCOD/(L∙d) and 0.74gCOD/(L∙d)/0.56gCOD/(L∙d) respectively. The fluctuation frequency of organic load was defined as the number of OLR high-low fluctuation every 12 days, R1’s OLR did not fluctuate, R2 and R3 completed OLR high-low fluctuation every 12 days and 4 days respectively, and their fluctuation frequencies were 0, 1 and 3 respectively. The results showed that the average particle sizes of R1, R2 and R3 were 318.86μm, 426.71μm and 593.06μm, respectively. The extracellular polymeric substance (EPS) content (VSS) were 71.97mg/g, 75.88mg/g and 80.35mg/g, PN/PS were 4.24, 5.14 and 5.72, respectively, indicating that the granules had higher hydrophobicity and stability when the fluctuation frequency was 3. In addition, the internal carbon storage rates (CODin) of R1, R2 and R3 were 97.06%, 98.37%, 98.91%, respectively, and the internal carbon storage rate (SND) efficiencies were 44.74%, 58.20%, 64.42%, respectively. The average total phosphorus (TP) removal efficiencies were 86.82%, 89.36% and 92.65%, respectively, the average total nitrogen (TN) removal efficiency were 71.69%, 74.31% and 78.55%, respectively, indicating that the granules had higher carbon source utilization efficiency and pollutant removal ability when the fluctuation frequency was 3.