Separation of lysozyme from egg white by electrodialysis with ultrafiltration membrane(EDUF) process

doi:10.16085/j.issn.1000-6613.2022-1293

Abstract

Abstract:

Lysozyme (LYZ) is a natural active protein with stable chemical properties, non-toxic and antibacterial function. Lysozyme with high purity and high activity has great value in the fields of food and medicine. In this paper, a novel electrodialysis with ultrafiltration membrane (EDUF) technology was used to directly separate LYZ from egg white based on the difference in charge and molecular weight between ovalbumin (OVA) and LYZ under specific pH conditions. The effects of ultrafiltration membrane material and the solution flow rates in feed and recovery compartments on the separation performance of EDUF were investigated. Results showed that the recovery rate and purity of LYZ could respectively reached 21.05% and 100% by using PVDF100Ⅰmembrane when the operating voltage was 5.0V and the solution flow rates in feed and recovery compartments were both 50mL/min. The final transport rate of LYZ was 0.21g/(m²·h) and energy consumption per unit product was 2.30kW·h/g under the tested conditions. This study demonstrates that EDUF, as an unique electro-driven membrane separation technology, has remarkable characteristics with its high selectivity. EDUF technology has good application prospects in the field of high-efficient separation of active proteins.

Key words: electrodialysis with ultrafiltration membrane (EDUF), lysozyme (LYZ), egg white, separation, purification, protein

摘要：

溶菌酶（LYZ）是一种化学性质稳定、无毒无害的具有抗菌作用的天然活性蛋白，高纯度、高活性溶菌酶在食品与医药领域具有重要价值。本文尝试采用新型电渗析／超滤内在耦合（EDUF）技术，同时利用特定pH条件下蛋清中卵白蛋白（OVA）与LYZ荷电性及分子量的差异，从蛋清中直接分离、提取LYZ。重点考察了超滤膜材料及EDUF原料室和回收室内料液流量对过程分离性能的影响。结果表明，在操作电压为5.0V，采用PVDF100Ⅰ超滤膜，原料室和回收室料液流量均为50mL/min时，LYZ的回收率可达21.05%，所回收LYZ的纯度可达100%，其最终迁移速率为0.21g/(m²·h)，单位产品能耗为2.30kW·h/g。该研究证明了EDUF作为一项独特的电驱动膜分离技术，具有选择性高的显著特点，在活性蛋白高效分离领域具有较好的应用前景。

关键词: 电渗析/超滤内在耦合, 溶菌酶, 蛋清, 分离, 纯化, 蛋白质

CLC Number:

X792

SUN Luqin, LU Huixia, WANG Jianyou. Separation of lysozyme from egg white by electrodialysis with ultrafiltration membrane(EDUF) process[J]. Chemical Industry and Engineering Progress, 2023, 42(5): 2262-2271.

孙鲁芹, 卢会霞, 王建友. 电渗析/超滤内在耦合过程分离蛋清中溶菌酶[J]. 化工进展, 2023, 42(5): 2262-2271.

Figures/Tables 10

References 21

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隔室	料液组分	流量/mL·min^-1
原料室	溶解于2g/L KCl中，OVA浓度为1g/L，LYZ浓度为0.1g/L的蛋白混合溶液	30~70
回收室	2g/L KCl	30~70
保护室	40g/L Na₂SO₄	200
电极室	20g/L Na₂SO₄	200

隔室	料液组分	流量/mL·min^-1
原料室	溶解于2g/L KCl中，OVA浓度为1g/L，LYZ浓度为0.1g/L的蛋白混合溶液	30~70
回收室	2g/L KCl	30~70
保护室	40g/L Na₂SO₄	200
电极室	20g/L Na₂SO₄	200

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