Isolation of halophilic bacterium and their decolorization characteristics and mechanism of azo dyes

doi:10.16085/j.issn.1000-6613.2022-1092

Abstract

Abstract:

High salinity in textile wastewater limited the application of biological method in textile wastewater. Isolation of halophilic microorganisms is important for improving the treatment efficiency of high salinity textile wastewater. A strain was isolated from active sludge of textile wastewater, which can decolorize metanil yellow. The bacteria were identified by 16S rDNA. The degradation mechanism was analyzed. The results showed the bacteria had the highest homology with Exiguobaterium strain ACCC11618 and belong to the genus. At 5% salinity, more than 95% metanil yellow was decolorized by S2 within 8h. The optimum decolorization condition was 5% salinity, pH 7, at 30℃, and yeast powder as carbon source. Azo reductase and NADH-DCIP are the main degrading enzymes. Salinity inhibits the activity of these two enzymes. The azo bond of metanil yellow was symmetrically broken into 4-aminobenzene sulfonic acid and p-aminobenzidine, which further degraded into diphenylamine, aniline, and 2-heptanone oxime. The toxicity was decreased after decolorization. The strain could decolorize metanil yellow at different concentrations, which showed good application potential. This study is expected to provide species resources and theoretical basis for treatment high salinity textile wastewater.

Key words: azo dye, separation, decolorization characteristics, metanil yellow G, halophilic

摘要：

高盐限制了普通微生物处理印染废水的效果，分离嗜盐微生物对于提高高盐印染废水的处理效率，具有重要的应用价值。本研究从印染废水的活性污泥中，分离了一株降解酸性金黄G的菌株，通过16S rDNA对该菌进行鉴定，并研究了其降解机理。结果表明，该菌与Exiguobaterium strain ACCC11618同源性最高，属于微小杆菌属。该菌在5%盐度下，8h内对100mg/L的酸性金黄G脱色95%以上。最佳脱色条件是30℃下，pH=7，5%盐度，以酵母粉作为碳源。偶氮还原酶、NADH-DCIP酶是主要的降解酶，盐度抑制了这两种酶的活性。酸性金黄G的偶氮键对称断裂成4-氨基苯磺酸和对氨基二苯胺，进一步降解为二苯胺、苯胺、2-庚酮肟等，降解后产物毒性降低。菌株对不同浓度的酸性金黄G具有耐受性，具有良好的应用潜力。该研究以期为嗜盐菌处理高盐印染废水提供菌种资源和理论依据。

关键词: 偶氮染料, 分离, 脱色, 酸性金黄G, 嗜盐菌

CLC Number:

X170

TIAN Fang, GUO Guang, DING Keqiang, YANG Feng, LIU Chong, WANG Huiya. Isolation of halophilic bacterium and their decolorization characteristics and mechanism of azo dyes[J]. Chemical Industry and Engineering Progress, 2023, 42(4): 2115-2121.

田芳, 郭光, 丁克强, 杨凤, 刘翀, 王慧雅. 降解偶氮染料嗜盐菌的分离、降解特性及机制[J]. 化工进展, 2023, 42(4): 2115-2121.

Figures/Tables 12

References 26

1	郭光, 田芳, 刘妍, 等. 中度嗜盐菌群对酸性大红GR的脱色性能研究[J]. 微生物学通报, 2017, 44(11): 2567-2574.
	GUO Guang, TIAN Fang, LIU Yan, et al. Decoloring acid scarlet GR by a moderately halophilic bacterial consortium[J]. Microbiology China, 2017, 44(11): 2567-2574.
2	ALSANTALI R I, RAJA Q A, ALZAHRANI A Y, et al. Miscellaneous azo dyes: A comprehensive review on recent advancements in biological and industrial applications[J]. Dyes and Pigments, 2022, 199: 110050.
3	张庆华, 陈国涛, 冯琳琳, 等. 混合菌群对偶氮染料的脱色降解研究进展[J]. 应用与环境生物学报, 2020, 26(2): 469-478.
	ZHANG Qinghua, CHEN Guotao, FENG Linlin, et al. Research progress on microbial decolorization and degradation of azo dyes[J]. Chinese Journal of Applied and Environmental Biology, 2020, 26(2): 469-478.
4	YASEEN D A, SCHOLZ M. Textile dye wastewater characteristics and constituents of synthetic effluents: A critical review[J]. International Journal of Environmental Science and Technology, 2019, 16(2): 1193-1226.
5	AJAZ Mehvish, SHAKEEL Sana, REHMAN Abdul. Microbial use for azo dye degradation—A strategy for dye bioremediation[J]. International Microbiology: the Official Journal of the Spanish Society for Microbiology, 2020, 23(2): 149-159.
6	TIAN Fang, WANG Yongbo, GUO Guang, et al. Enhanced azo dye biodegradation at high salinity by a halophilic bacterial consortium[J]. Bioresource Technology, 2021, 326: 124749.
7	TIAN Fang, GUO Guang, ZHANG Can, et al. Isolation, cloning and characterization of an azoreductase and the effect of salinity on its expression in a halophilic bacterium[J]. International Journal of Biological Macromolecules, 2019, 123: 1062-1069.
8	GIOVANELLA Patricia, VIEIRA Gabriela A L, RAMOS OTERO Igor V, et al. Metal and organic pollutants bioremediation by extremophile microorganisms[J]. Journal of Hazardous Materials, 2020, 382: 121024.
9	MONTAÑEZ-BARRAGÁN B, SANZ-MARTÍN J L, GUTIÉRREZ-MACÍAS P, et al. Azo dyes decolorization under high alkalinity and salinity conditions by Halomonas sp. in batch and packed bed reactor[J]. Extremophiles: Life Under Extreme Conditions, 2020, 24(2): 239-247.
10	GUO Guang, HAO Jiuxiao, TIAN Fang, et al. Decolorization of Metanil Yellow G by a halophilic alkalithermophilic bacterial consortium[J]. Bioresource Technology, 2020, 316: 123923.
11	RATHOUR Rohit, JAIN Kunal, MADAMWAR Datta, et al. Microaerophilic biodegradation of raw textile effluent by synergistic activity of bacterial community DR4[J]. Journal of Environmental Management, 2019, 250: 109549.
12	张莹, 石萍, 马炯. 微小杆菌Exiguobacterium spp.及其环境应用研究进展[J]. 应用与环境生物学报, 2013, 19(5): 898-904.
	ZHANG Ying, SHI Ping, MA Jiong. Exiguobacterium spp. and their applications in environmental remediation[J]. Chinese Journal of Applied and Environmental Biology, 2013, 19(5): 898-904.
13	TAN Liang, QU Yuanyuan, ZHOU Jiti, et al. Decolorization of azo dyes by a newly isolated Exiguobacterium sp. strain TL under high salt condition[J]. Journal of Biotechnology, 2008, 136: S688.
14	DHANVE R S, SHEDBALKAR U U, JADHAV J P. Biodegradation of diazo reactive dye Navy blue HE2R (Reactive blue 172) by an isolated Exiguobacterium sp. RD3[J]. Biotechnology and Bioprocess Engineering, 2008, 13(1): 53-60.
15	刘娜, 谢学辉, 王钰, 等. 细菌利用不同碳、氮源共代谢降解脱色偶氮染料研究进展[J]. 微生物学通报, 2019, 46(5): 1185-1195.
	LIU Na, XIE Xuehui, WANG Yu, et al. Carbon and nitrogen co-metabolism during bacterial degradation and decolorization of azo dyes[J]. Microbiology China, 2019, 46(5): 1185-1195.
16	IMRAN Muhammad, ARSHAD Muhammad, NEGM Fayek, et al. Yeast extract promotes decolorization of azo dyes by stimulating azoreductase activity in Shewanella sp. strain IFN4[J]. Ecotoxicology and Environmental Safety, 2016, 124: 42-49.
17	SAMUCHIWAL Saurabh, GOLA Deepak, MALIK Anushree. Decolourization of textile effluent using native microbial consortium enriched from textile industry effluent[J]. Journal of Hazardous Materials, 2021, 402: 123835.
18	GUO Guang, LIU Chong, HAO Jiuxiao, et al. Development and characterization of a halo-thermophilic bacterial consortium for decolorization of azo dye[J]. Chemosphere, 2021, 272: 129916.
19	GUO Guang, TIAN Fang, ZHANG Can, et al. Performance of a newly enriched bacterial consortium for degrading and detoxifying azo dyes[J]. Water Science and Technology: a Journal of the International Association on Water Pollution Research, 2019, 79(11): 2036-2045.
20	VARJANI S, RAKHOLIYA P, NG H Y, et al. Microbial degradation of dyes: an overview[J]. Bioresource Technology, 2020, 314: 123728..
21	SELVARAJ V, Swarna KARTHIKA T, MANSIYA C, et al. An over review on recently developed techniques, mechanisms and intermediate involved in the advanced azo dye degradation for industrial applications[J]. Journal of Molecular Structure, 2021, 1224: 129195.
22	LIU Weijie, LIU Cong, LIU Liang, et al. Simultaneous decolorization of sulfonated azo dyes and reduction of hexavalent chromium under high salt condition by a newly isolated salt-tolerant strain Bacillus circulans BWL1061[J]. Ecotoxicology and Environmental Safety, 2017, 141: 9-16.
23	VATANDOOSTARANI Simin, BAGHERI LOTFABAD Tayebe, HEIDARINASAB Amir, et al. Degradation of azo dye methyl red by Saccharomyces cerevisiae ATCC 9763[J]. International Biodeterioration & Biodegradation, 2017, 125: 62-72.
24	CHEN Yan, FENG Linlin, LI Hanguang, et al. Biodegradation and detoxification of Direct Black G textile dye by a newly isolated thermophilic microflora[J]. Bioresource Technology, 2018, 250: 650-657.
25	范娇, 谢学辉, 秦艳, 等. 功能菌群DDMZ1共代谢脱色降解不同结构染料差异效应[J]. 化工进展, 2022, 41(8): 4449-4463.
	FAN Jiao, XIE Xuehui, QIN Yan, et al. Differential effect of DDMZ1 cometabolic decolorization on the degradation of dyes with different structures[J]. Chemical Industry and Engineering Progress, 2022, 41(8): 4449-4463.
26	URBANIAK Monika, PRZYSTAS Wioletta, Ewa ZABLOCKA-GODLEWSKA, et al. Decolorization of azo and triphenylmethane dyes by MW113 Beauveria bassiana strain[J]. Desalination and Water Treatment, 2018, 136: 422-432.

酶的种类	不同盐度下的酶活性
酶的种类	1%	3%	5%	7%	9%
漆酶/µmol·mg^-1·min^-1	0.031	0.75	0.71	0.21	0.10
锰过氧化物酶/µmol·mg^-1·min^-1	0.078	0.083	0.084	0.082	0.078
偶氮还原酶/µmol·mg^-1·min^-1	0.29	1.05	1.82	1.23	0.136
NADH-DCIP/µg^-1·mg ^-1 ·min^-1	5.22	10.45	16.08	4.34	1.182

酶的种类	不同盐度下的酶活性
酶的种类	1%	3%	5%	7%	9%
漆酶/µmol·mg^-1·min^-1	0.031	0.75	0.71	0.21	0.10
锰过氧化物酶/µmol·mg^-1·min^-1	0.078	0.083	0.084	0.082	0.078
偶氮还原酶/µmol·mg^-1·min^-1	0.29	1.05	1.82	1.23	0.136
NADH-DCIP/µg^-1·mg ^-1 ·min^-1	5.22	10.45	16.08	4.34	1.182

项目	黄瓜			水稻
项目	发芽率/%	胚芽/cm	胚根/cm	发芽率/%	胚芽/cm	胚根/cm
水	100	5.7±0.8	4.9±0.6	100	4.8±0.9	3.5±0.5
酸性金黄G	46.6	3.9±0.6	3.2±0.7	66.6	3.1±0.6	2.4±0.5
降解产物	100	5.0±0.7	4.5±0.9	100	4.7±0.7	3.3±0.6

项目	黄瓜			水稻
项目	发芽率/%	胚芽/cm	胚根/cm	发芽率/%	胚芽/cm	胚根/cm
水	100	5.7±0.8	4.9±0.6	100	4.8±0.9	3.5±0.5
酸性金黄G	46.6	3.9±0.6	3.2±0.7	66.6	3.1±0.6	2.4±0.5
降解产物	100	5.0±0.7	4.5±0.9	100	4.7±0.7	3.3±0.6

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