化工进展 ›› 2021, Vol. 40 ›› Issue (9): 5222-5230.DOI: 10.16085/j.issn.1000-6613.2021-0263

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二乙醇胺强化胶球藻Coccomyxa subellipsoidea C-169固定CO2和积累油脂

邹帅(), 李玉芹(), 马怡然, 齐振华, 贾权威   

  1. 湘潭大学化工学院,湖南 湘潭 411105
  • 收稿日期:2021-02-02 修回日期:2021-03-30 出版日期:2021-09-05 发布日期:2021-09-13
  • 通讯作者: 李玉芹
  • 作者简介:邹帅(1994—),男,硕士研究生,研究方向为生物质能源。E-mail:573145729@qq.com
  • 基金资助:
    国家自然科学基金(21676228);湖南省教育厅重点项目(18A068)

Diethanolamine strengthening CO2 fixation and lipid accumulation in Coccomyxa subellipsoidea C-169

ZOU Shuai(), LI Yuqin(), MA Yiran, QI Zhenhua, JIA Quanwei   

  1. College of Chemical Engineering, Xiangtan University, Xiangtan 411105, Hunan, China
  • Received:2021-02-02 Revised:2021-03-30 Online:2021-09-05 Published:2021-09-13
  • Contact: LI Yuqin

摘要:

以优良藻株胶球藻Coccomyxa subellipsoidea C-169为受试对象,探究不同二乙醇胺(DEA)浓度对胶球藻固定CO2及积累油脂的影响。结果表明,适宜浓度DEA可显著提高胶球藻固定CO2和积累油脂效率。在40mg/L DEA条件下,胶球藻生物量、CO2固定效率、油脂含量、油脂产率均达到最大值为0.97g/L、225.98mg/(L·d)、64.33%、59.9mg/(L·d),分别较无DEA对照提高了1.64倍、1.64倍、1.15倍和1.27倍。外源DEA作用提高了体系气液传质系数,增强CO2传质效率,使得胶球藻可用无机碳源比例增加,同时上调固碳路径Rubisco和CA加强胶球藻固定CO2,上调脂肪酸路径ACCase以及下调ACCase竞争路径PEPC为油脂合成积累提供前体和能量,从而协同强化了胶球藻固定CO2和积累油脂。本文研究结果为工业化胶球藻CO2生物减排及可再生能源开发提供依据。

关键词: 二乙醇胺, 生物质, 二氧化碳, 油脂, 生物柴油

Abstract:

The effects of different diethanolamine concentrations on growth, CO2 biofixation, and lipid accumulation of eminent microalga Coccomyxa subellipsoidea C-169 was explored. The results showed that the appropriate concentration of DEA could significantly improve CO2 biofixation and lipid accumulation efficiency. The highest levels of biomass, CO2 biofixation rate, lipid content and lipid productivity were achieved to 0.97g/L, 225.98mg/(L·d), 64.33%, and 59.9mg/(L·d) under 40mg/L DEA, respectively, which were separately 1.64-, 1.64-, 1.15- and 1.27-fold more than that without DEA group. Exogenous DEA improved gas-liquid mass transfer coefficient and CO2 mass transfer efficiency, and consequently enhanced the proportion of available inorganic carbon sources for C. subellipsoidea. Synchronously, exogenous DEA up-regulated 1,5-bisphosphate carboxylase Rubisco and carbonic anhydrase CA in carbon fixation pathway, and consequently improved CO2 bio-fixation of C. subellipsoidea. DEA also up-regulated acetyl-coa carboxylase ACCase in lipid pathway and down-regulated pyruvate carboxylase PEPC in ACCase competition pathway to strengthen lipid accumulation in C. subellipsoidea. These results provided a basis for industrial CO2 emission reduction and renewable energy development of C. subellipsoidea.

Key words: diethanolamine, biomass, carbon dioxide, lipids, biodiesel

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