二乙醇胺强化胶球藻Coccomyxa subellipsoidea C-169固定CO2和积累油脂

doi:10.16085/j.issn.1000-6613.2021-0263

摘要/Abstract

摘要：

以优良藻株胶球藻Coccomyxa subellipsoidea C-169为受试对象，探究不同二乙醇胺（DEA）浓度对胶球藻固定CO₂及积累油脂的影响。结果表明，适宜浓度DEA可显著提高胶球藻固定CO₂和积累油脂效率。在40mg/L DEA条件下，胶球藻生物量、CO₂固定效率、油脂含量、油脂产率均达到最大值为0.97g/L、225.98mg/(L·d)、64.33%、59.9mg/(L·d)，分别较无DEA对照提高了1.64倍、1.64倍、1.15倍和1.27倍。外源DEA作用提高了体系气液传质系数，增强CO₂传质效率，使得胶球藻可用无机碳源比例增加，同时上调固碳路径Rubisco和CA加强胶球藻固定CO₂，上调脂肪酸路径ACCase以及下调ACCase竞争路径PEPC为油脂合成积累提供前体和能量，从而协同强化了胶球藻固定CO₂和积累油脂。本文研究结果为工业化胶球藻CO₂生物减排及可再生能源开发提供依据。

关键词: 二乙醇胺, 生物质, 二氧化碳, 油脂, 生物柴油

Abstract:

The effects of different diethanolamine concentrations on growth, CO₂ biofixation, and lipid accumulation of eminent microalga Coccomyxa subellipsoidea C-169 was explored. The results showed that the appropriate concentration of DEA could significantly improve CO₂ biofixation and lipid accumulation efficiency. The highest levels of biomass, CO₂ biofixation rate, lipid content and lipid productivity were achieved to 0.97g/L, 225.98mg/(L·d), 64.33%, and 59.9mg/(L·d) under 40mg/L DEA, respectively, which were separately 1.64-, 1.64-, 1.15- and 1.27-fold more than that without DEA group. Exogenous DEA improved gas-liquid mass transfer coefficient and CO₂ mass transfer efficiency, and consequently enhanced the proportion of available inorganic carbon sources for C. subellipsoidea. Synchronously, exogenous DEA up-regulated 1,5-bisphosphate carboxylase Rubisco and carbonic anhydrase CA in carbon fixation pathway, and consequently improved CO₂ bio-fixation of C. subellipsoidea. DEA also up-regulated acetyl-coa carboxylase ACCase in lipid pathway and down-regulated pyruvate carboxylase PEPC in ACCase competition pathway to strengthen lipid accumulation in C. subellipsoidea. These results provided a basis for industrial CO₂ emission reduction and renewable energy development of C. subellipsoidea.

Key words: diethanolamine, biomass, carbon dioxide, lipids, biodiesel

中图分类号:

邹帅, 李玉芹, 马怡然, 齐振华, 贾权威. 二乙醇胺强化胶球藻Coccomyxa subellipsoidea C-169固定CO₂和积累油脂[J]. 化工进展, 2021, 40(9): 5222-5230.

ZOU Shuai, LI Yuqin, MA Yiran, QI Zhenhua, JIA Quanwei. Diethanolamine strengthening CO₂ fixation and lipid accumulation in Coccomyxa subellipsoidea C-169[J]. Chemical Industry and Engineering Progress, 2021, 40(9): 5222-5230.

图/表 6

图1 胶球藻固定CO2和积累油脂实验简易装置1—减压阀；2—流量计；3—气体混合器；4—气体滤菌膜；5—LED灯；6—pH计；7—取样加样口；8—六通道生物反应器

图2 不同CO2体积分数对胶球藻生物量及固定CO2和积累油脂的影响

图3 不同DEA浓度对胶球藻固定CO2及积累油脂的影响

图4 不同DEA浓度对胶球藻细胞超微结构的影响LP—油脂滴；ST—淀粉颗粒

图5 二乙醇胺对胶球藻碳酸酐酶、核酮糖二磷酸羧化酶、磷酸烯醇式丙酮酸羧化酶、乙酰辅酶 A 羧化酶活性影响

表1 二乙醇胺作用对胶球藻细胞脂肪酸组分的影响

脂肪酸组成	相对含量/%
脂肪酸组成	2% CO₂	40mg/L DEA+2% CO₂
棕榈酸（C₁₆∶0）	41.78	26.13
硬脂酸（C₁₈∶0）	17.25	7.23
油酸（C₁₈∶1）	11.59	56.65
亚油酸（C₁₈∶2）	12.39	—
亚麻酸（C₁₈∶3）	—	1.99
饱和脂肪酸（SFAs）	76.03	41.36
单不饱和脂肪酸（MUFAs）	11.59	56.65
多不饱和脂肪酸（PUFAs）	23.97	1.99

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二乙醇胺强化胶球藻Coccomyxa subellipsoidea C-169固定CO₂和积累油脂

Diethanolamine strengthening CO₂ fixation and lipid accumulation in Coccomyxa subellipsoidea C-169

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