反硝化除磷的影响因素及聚磷菌与聚糖菌耦合新工艺的研究进展

doi:10.16085/j.issn.1000-6613.2020-0179

摘要/Abstract

摘要：

反硝化除磷（denitrifying phosphorus removal，DPR）工艺较传统脱氮除磷工艺具有节省曝气能耗、高效利用碳源、低污泥产量等优点而得到广泛的研究。本文综述了近年来在这一领域的研究进展，包括反硝化除磷菌（DPAOs）微生物学、碳源种类、pH、亚硝酸盐浓度及游离亚硝酸（free nitrous acid，FNA）、污泥龄（sludge retention time，SRT）、C/P比及mgNO $x -$ -N/mgPO $4 3 -$ -P、聚糖菌（GAOs）等。大多数研究只关注了聚磷菌（PAOs）和GAOs之间的竞争关系，而通过GAOs作用的内碳源部分反硝化（endogenous partial-denitrification，EPD），能够将NO $3 -$ -N转化为NO $2 -$ -N，将进一步降低同步脱氮除磷对碳源的需求。反硝化除磷的新工艺符合我国低C/N值的污水现状，SNADPR工艺是将部分硝化、厌氧氨氧化、反硝化与反硝化除磷相结合的先进脱氮除磷工艺，Anammox-EPDPR工艺协同厌氧氨氧化、EPD和反硝化除磷，充分利用GAOs内碳源的代谢作用，以产生NO $2 -$ -N，减轻DPAOs和anammox菌对电子受体的竞争。以NO $2 -$ -N为电子受体的短程反硝化除磷与新型脱氮工艺的耦合将成为实现污水高效节能的同步脱氮除磷的新方向。

关键词: 反硝化除磷, 影响因素, 聚磷菌, 聚糖菌, 内源部分反硝化

Abstract:

Denitrifying phosphorus removal (DPR) process has been widely studied for its advantages of energy saving, high efficiency utilization of carbon source and low sludge yield. This paper assesses the recent advances in this field, particularly relating to denitrifying phosphorus accumulating organisms (DPAOs) microbiology,the types of carbon source, pH value, nitrite concentration, free nitrous acid (FNA), sludge retention time (SRT), C/P, mgNO $x -$ -N/mgPO $4 3 -$ -P and glycogen accumulating organisms (GAOs).Previous research in this field only focuses on the competitive relationship between phosphorus accumulating organisms (PAOs) and GAOs. However, through endogenous partial-denitrification (EPD) driven by GAOs, NO $3 -$ -N can be converted into NO $2 -$ -N, which will further reduce the demand of carbon source for simultaneous nitrogen and phosphorus treatment. The novel process of denitrifying phosphorus removal accords with the current situation of low C/N value sewage in China. SNADPR process is an advanced simultaneous nitrogen and phosphorus removal process combining partial nitrification, anammox and denitrification (SNAD) with denitrifying phosphorus removal. Anammox-EPDPR process combining anammox, endogenous partial-denitrification and denitrifying phosphorus removal, makes full use of GAOs on intracellular carbon source for NO $2 -$ -N production and alleviates competition between DPAOs and anammox for electron acceptor. The coupling of new denitrification process and partial denitrifying phosphorus removal process utilizing NO $2 -$ -N as electron acceptorwill become a new direction of simultaneous nitrogen and phosphorus removal treatment with high efficiency and energy saving.

Key words: denitrifying phosphorus removal, inhibitory factors, phosphorus accumulating organisms, glycogen accumulating organisms, endogenous partial-denitrification

中图分类号:

X703

韦佳敏, 刘文如, 程洁红, 沈耀良. 反硝化除磷的影响因素及聚磷菌与聚糖菌耦合新工艺的研究进展[J]. 化工进展, 2020, 39(11): 4608-4618.

Jiamin WEI, Wenru LIU, Jiehong CHENG, Yaoliang SHEN. Influencing factors of denitrifying phosphorus removal and advance research on novel process of coupling PAOs and GAOs[J]. Chemical Industry and Engineering Progress, 2020, 39(11): 4608-4618.

参考文献

1	KUBA T, SMOLDERS G, LOOSDRECHT M C M V, et al. Biological phosphorus removal from wastewater by anaerobic-anoxic sequencing batch reactor[J]. Waterence and Technology, 1993, 27(5/6): 241-252.
2	BAEZA J A, GABRIEL D, LAFUENTE J. Improving the nitrogen removal efficiency of an A²/O based WWTP by using an on-line knowledge based expert system[J]. Water Research, 2002, 36(8): 2109-2123.
3	XU X Y, LIU G, ZHU L. Enhanced denitrifying phosphorous removal in a novel anaerobic/aerobic/anoxic (AOA) process with the diversion of internal carbon source[J]. Bioresource Technology, 2011, 102(22): 10340-10345.
4	ZHOU Y, PIJUAN M, YUAN Z G. Development of a 2-sludge, 3-stage system for nitrogen and phosphorous removal from nutrient-rich wastewater using granular sludge and biofilms[J]. Water Research, 2008, 42(12): 3207-3217.
5	KUBA T, LOOSDRECHT M C M V, BRANDSE F A, et al. Occurrence of denitrifying phosphorus removing bacteria in modified UCT-type wastewater treatment plants[J]. Water Research, 1997, 31(4): 777-786.
6	叶丽红, 李冬, 张杰, 等. 亚硝化-反硝化除磷技术研究进展[J]. 北京工业大学学报, 2016,42(4): 585-593.
6	YE L H, LI D, ZHANG J, et al. Advance of research on the technology of nitrite-denitrifying phosphorus removal[J]. Journal of Beijin University of Technology, 2016,42(4): 585-593.
7	章璋, 朱易春, 王佳琪, 等. 短程反硝化除磷的影响因素分析[J]. 江西理工大学学报, 2019,40(1): 46-53.
7	ZHANG Z, ZHU Y C, WANG J Q, et al. Analysis of influential factors of partial denitrifying dephosphatation[J]. Journal of Jiangxi University of Science and Technology, 2019, 40(1): 46-53.
8	KONG Y, NIELSEN J L, NIELSEN P H. Microautoradiographic study of rhodocyclus-related polyphosphate-accumulating bacteria in full-scale enhanced biological phosphorus removal plants[J]. Applied and Environmental Microbiology, 2004, 70(9): 5383-5390.
9	JEON C O, LEE D S, PARK J M. Microbial communities in activated sludge performing enhanced biological phosphorus removal in a sequencing batch reactor[J]. Water Research, 2003, 37(9): 2195-2205.
10	HU J Y, ONG S L, NG W J, et al. A new method for characterizing denitrifying phosphorus removal bacteria by using three different types of electron acceptors[J]. Water Research, 2003, 37(14): 3463-3471.
11	DABERT P, FLEURAT-LESSARD A, MOUNIER E, et al. Monitoring of the microbial community of a sequencing batch reactor bioaugmented to improve its phosphorus removal capabilities[J]. Water Science and Technology, 2001, 43(3): 1-8.
12	JABARI P, MUNZ G, OLESZKIEWICZ J A. Selection of denitrifying phosphorous accumulating organisms in IFAS systems: comparison of nitrite with nitrate as an electron acceptor[J]. Chemosphere, 2014, 109: 20-27.
13	OEHMEN A, LEMOS P C, CARVALHO G, et al. Advances in enhanced biological phosphorus removal: from micro to macro scale[J]. Water Research, 2007, 41(11): 2271-2300.
14	GUISASOLA A, QURIE M, VARGAS M D M, et al. Failure of an enriched nitrite-DPAO population to use nitrate as an electron acceptor[J]. Process Biochemistry, 2009, 44(7): 689-695.
15	CARVALHO G, LEMOS P C, OEHMEN A, et al. Denitrifying phosphorus removal: linking the process performance with the microbial community structure[J]. Water Research, 2007, 41(19): 4383-4396.
16	RUBIO-RINCóN F J, LOPEZ-VAZQUEZ C M, WELLES L, et al. Cooperation between Candidatus competibacter and Candidatus accumulibacter clade Ⅰ, in denitrification and phosphate removal processes[J]. Water Research, 2017, 120: 156-164.
17	MARTIN H G, IVANOVA N, KUNIN V, et al. Metagenomic analysis of two enhanced biological phosphorus removal (EBPR) sludge communities[J]. Nature Biotechnology, 2006, 24(10): 1263-1269.
18	HE S, GALL D L, MCMAHON K D. Candidatus accumulibacter population structure in enhanced biological phosphorus removal sludges as revealed by polyphosphate kinase genes[J]. Applied and Environmental Microbiology, 2007, 73(18): 5865-5874.
19	PETERSON S B, WARNECKE F, MADEJSKA J, et al. Environmental distribution and population biology of Candidatus accumulibacter, a primary agent of biological phosphorus removal[J]. Environmental Microbiology, 2008, 10(10): 2692-2703.
20	OEHMEN A, CARVALHO G, LOPEZ-VAZQUEZ C M, et al. Incorporating microbial ecology into the metabolic modelling of polyphosphate accumulating organisms and glycogen accumulating organisms[J]. Water Research, 2010, 44(17): 4992-5004.
21	CAMEJO P Y, OWEN B R, MARTIRANO J, et al. Candidatus accumulibacter phosphatis clades enriched under cyclic anaerobic and microaerobic conditions simultaneously use different electron acceptors[J]. Water Research, 2016, 102: 125-137.
22	MAO Y, GRAHAM D W, TAMAKI H, et al. Dominant and novel clades of Candidatus accumulibacter phosphatis in 18 globally distributed full-scale wastewater treatment plants[J]. Scientific Reports, 2015, 5(11857): 1-10.
23	OEHMEN A, CARVALHO G, FREITAS F, et al. Assessing the abundance and activity of denitrifying polyphosphate accumulating organisms through molecular and chemical techniques[J]. Water Science and Technology, 2010, 61(8): 2061-2068.
24	ZENG W, BAI X, GUO Y, et al. Interaction of Candidatus accumulibacter and nitrifying bacteria to achieve energy-efficient denitrifying phosphorus removal via nitrite pathway from sewage[J]. Enzyme and Microbial Technology, 2017, 105: 1-8.
25	SAAD S A, WELLES L, ABBAS B, et al. Denitrification of nitrate and nitrite by Candidatus accumulibacter phosphati' clade IC[J]. Water Research, 2016, 105: 97-109.
26	ZENG R J, YUAN Z, KELLER J. Effects of solids concentration, pH and carbon addition on the production rate and composition of volatile fatty acids in prefermenters using primary sewage sludge[J]. Water Science and Technology, 2006, 53(8): 263-269.
27	ZHANG S H, HUANG Y, HUA Y M. Denitrifying dephosphatation over nitrite: Effects of nitrite concentration, organic carbon and pH[J]. Bioresource Technology, 2010, 101(11): 3870-3875.
28	吴昌永，彭永臻，彭轶，等. 碳源类型对A²O系统脱氮除磷的影响[J].环境科学, 2009, 30(3): 798-802.
28	WU C Y, PENG Y Z, PENG Y, et al. Influence of carbon source on biological nutrient removal in A²O process[J]. Environmental Science, 2009, 30(3): 798-802.
29	YUN G, LEE H, HONG Y, et al. The difference of morphological characteristics and population structure in PAO and DPAO granular sludges[J]. Journal of Environmental Sciences, 2019, 76: 388-402.
30	PIJUAN M, SAUNDERS A M, GUISASOLA A, et al. Enhanced biological phosphorus removal in a sequencing batch reactor using propionate as the sole carbon source[J]. Biotechnology and Bioengineering, 2004, 85(1): 56-67.
31	MEYER R L, SAUNDERS A M, BLACKALL L L. Putative glycogen-accumulating organisms belonging to the Alphaproteobacteria identified through rRNA-based stable isotope probing[J]. Microbiology, 2006, 152(2): 419-429.
32	OEHMEN A, YUAN Z, BLACKALL L L, et al. Comparison of acetate and propionate uptake by polyphosphate accumulating organisms and glycogen accumulating organisms[J]. Biotechnology and Bioengineering, 2005, 91(2): 162-168.
33	TAYA C, GARLAPATI V K, GUISASOLA A, et al. The selective role of nitrite in the PAO/GAO competition[J]. Chemosphere, 2013, 93(4): 612-618.
34	LU H, OEHMEN A, VIRDIS B, et al. Obtaining highly enriched cultures of Candidatus accumulibacter phosphatis through alternating carbon sources[J].Water Research, 2007, 40(20): 3838-3848.
35	LOPEZ-VAZQUEZ C M, OEHMEN A, HOOIJMANS C M, et al. Modeling the PAO–GAO competition: effects of carbon source, pH and temperature[J]. Water Research, 2009, 43(2): 450-462.
36	WANG Y, PENG Y, STEPHENSON T. Effect of influent nutrient ratios and hydraulic retention time (HRT) on simultaneous phosphorus and nitrogen removal in a two-sludge sequencing batch reactor process[J]. Bioresour Technology, 2009, 100(14): 3506-3512.
37	彭永臻. SBR 法污水生物脱氮除磷及过程控制[M]. 北京: 科学出版社, 2011: 188-190.
37	PENG Y Z. The biological removal of nitrogen and phosphorus in wastewater by SBR[M]. Beijing: Science Press, 2011: 188-190.
38	胡筱敏, 李微, 刘金亮, 等. pH对以亚硝酸盐为电子受体反硝化除磷的影响[J]. 中南大学学报(自然科学版), 2013, 44(5): 2144-2149.
38	HU X M, LI W, LIU J L, et al.Influence of pH on denitrifying phosphorus removal using nitrite as electron acceptor [J].Journal of Central South University (Science and Technology), 2013, 44(5): 2144-2149.
39	LI W, ZHANG H Y, SUN H Z, et al. Influence of pH on short-cut denitrifying phosphorus removal[J]. Water Science and Engineering, 2018, 11(1): 17-22.
40	韦佳敏, 黄慧敏, 程诚, 等. 污泥龄及pH值对反硝化除磷工艺效能的影响[J]. 环境科学, 2019, 40(4): 382-387.
40	WEI J M, HUANG H M, CHENG C, et al. Effect of sludge retention time and pH on denitrifying phosphorus removal process[J]. Environmental Science, 2019, 40(4): 382-387.
41	FILIPE C, DAIGGER G T, GRADY L. pH as a key factor in the competition between glycogen-accumulating organisms and phosphorus-accumulating organisms[J]. Water Environment Research, 2001, 73(2): 223-232.
42	OEHMEN A, VIVES M T, LU H, et al. The effect of pH on the competition between polyphosphate -accumulating organisms and glycogen-accumulating organisms[J].Water Research, 2005, 39(15): 3727- 3737.
43	MEINHOLD J, ARNOLD E, ISAACS S. Effect of nitrite on anoxic phosphate uptake in biological phosphorus removal activated sludge[J]. Water Research, 1999, 33(8): 1871-1883.
44	AHN J, DAIDOU T, TSUNEDA S, et al. Metabolic behavior of denitrifying phosphate-accumulating organisms under nitrate and nitrite electron acceptor conditions[J]. Journal of Bioscience and Bioengineering, 2001, 92(5): 442-446.
45	王爱杰, 吴丽红, 任南琪, 等. 亚硝酸盐为电子受体反硝化除磷工艺的可行性[J]. 中国环境科学, 2005, 25(5): 515-518.
45	WANG A J,WU L H,REN N Q, et al. Feasibility of denitrifying phosphorus removal technique using nitrite as electron acceptor[J].China Environmental Science, 2005, 25(5): 515-518.
46	ZHOU S, ZHANG X, FENG L. Effect of different types of electron acceptors on the anoxic phosphorus uptake activity of denitrifying phosphorus removing bacteria[J]. Bioresource Technology, 2010, 101(6): 1603-1610.
47	DUAN H, GAO S, LI X, et al.Improving wastewater management using free nitrous acid (FNA)[J]. Water Research, 2020, 171: 115382.
48	ZHOU Y, PIJUAN M, YUAN Z. Free nitrous acid inhibition on anoxic phosphorus uptake and denitrification by poly-phosphate accumulating organisms[J]. Biotechnology and Bioengineering, 2007, 98(4): 903-912.
49	ZHOU Y, OEHMEN A, LIM M, et al. The role of nitrite and free nitrous acid (FNA) in wastewater treatment plants[J]. Water Research, 2011, 45(15): 4672-4682.
50	ZHOU Y, GANDA L, LIM M, et al. Free nitrous acid (FNA) inhibition on denitrifying poly-phosphate accumulating organisms (DPAOs)[J]. Applied Microbiology and Biotechnology, 2010, 88(1): 359-369.
51	WANG Y, GENG J, REN Z, et al. Effect of anaerobic reaction time on denitrifying phosphorus removal and N₂O production[J]. Bioresource Technology, 2011, 102(10): 5674-5684.
52	ZENG W, WANG A, ZHANG J, et al. Enhanced biological phosphate removal from wastewater and clade-level population dynamics of “Candidatus accumulibacter phosphatis” under free nitrous acid inhibition: linked with detoxication[J]. Chemical Engineering Journal, 2016, 296: 234-242.
53	PIJUAN M, YE L, YUAN Z. Free nitrous acid inhibition on the aerobic metabolism of poly-phosphate accumulating organisms[J]. Water Research, 2010, 44(20): 6063-6072.
54	ZHOU Y, GANDA L, LIM M, et al. Response of poly-phosphate accumulating organisms to free nitrous acid inhibition under anoxic and aerobic conditions[J]. Bioresource Technology, 2012, 116: 340-347.
55	BELLI T J, BEMARDELLI J K, COSTA R E, et al. Effect of solids retention time on nitrogen and phosphorus removal from municipal wastewater in a sequencing batch membrane bioreactor[J]. Environmental Technology, 2016, 38(7): 806-815.
56	ERSU C B, ONG S K, ARSLANKAYA E, et al. Impact of solids residence time on biological nutrient removal performance of membrane bioreactor[J]. Water Research, 2010, 44(10): 3192-3202.
57	ZHENG X, SUN P, HAN J, et al. Inhibitory factors affecting the process of enhanced biological phosphorus removal (EBPR)： a mini-review[J]. Process Biochemistry, 2014, 49(12): 2207-2213.
58	MERZOUKI M, BERNET N, DELGENES J P, et al. Biological denitrifying phosphorus removal in SBR: effect of added nitrate concentration and sludge retention time[J]. Water Science and Technology, 2001, 43(3): 191-194.
59	王朝朝, 闫立娜, 李思敏, 等. SRT对UCT-MBR反硝化除磷性能与膜污染行为的影响[J].中国环境科学, 2016, 36(6): 1715-1723.
59	WANG Z Z, YAN L N, LI S M, et al. Influence of sludge retention time on denitrifying dephosphatation propensity and membrane fouling behavior in a UCT-MBR process[J]. China Environmental Science,2016,36(6): 1715-1723.
60	MINO T, LOOSDRECHT M C M V, HEIJNEN J J. Microbiology and biochemistry of the enhanced biological phosphate removal process[J]. Water Research, 1998, 32(11): 3193-3207.
61	WANG Y, GENG J, REN Z, et al. Effect of COD/N and COD/P ratios on the PHA transformation and dynamics of microbial community structure in a denitrifying phosphorus removal process[J]. Journal of Chemical Technology and Biotechnology, 2013, 88(7): 1228-1236.
62	WANG Y, PENG Y, PENG C, et al. Influence of ORP variation, carbon source and nitrate concentration on denitrifying phosphorus removal by DPB sludge from dephanox process[J]. Water Science and Technology, 2004, 50(10): 153-161.
63	张建华, 彭永臻, 张淼, 等. 不同电子受体配比对反硝化除磷特性及内碳源转化利用的影响[J]. 化工学报, 2015, 66(12): 5045-5053.
63	ZHANG J H, PENG Y Z, ZHANG M, et al.Effect of different electron acceptor ratios on removal of nitrogen and phosphorus and conversion and utilization of internal carbon source[J]. CIESC Journal, 2015, 66(12): 5045-5053.
64	张建华，王淑莹，张淼，等. 不同反应时间内碳源转化对反硝化除磷的影响[J]. 中国环境科学, 2017,37(3): 989-997.
64	ZHANG J H, WANG S Y, ZHANG M, et al.Effect of conversion of internal carbon source on denitrifying phosphorus removal under different reaction time[J]. China Environmental Science,2017,37(3): 989-997.
65	ZHANG M, PENG Y, WANG C, et al. Optimization denitrifying phosphorus removal at different hydraulic retention times in a novel anaerobic anoxic oxic-biological contact oxidation process[J]. Biochemical Engineering Journal, 2016,106: 26-36.
66	COMA M, PUIG S, BALAGUER M D, et al. The role of nitrate and nitrite in a granular sludge process treating low-strength wastewater[J]. Chemical Engineering Journal, 2010, 164(1): 208-213.
67	LYU X, SHAO M, LI C, et al. Operation performance and microbial community dynamics of phosphorus removal sludge with different electron acceptors[J]. Journal of Industrial Microbiology and Biotechnology, 2014, 41(7): 1099-1108.
68	RIBERA-GUARDIA A, MARQUES R, ARANGIO C, et al. Distinctive denitrifying capabilities lead to differences in N₂O production by denitrifying polyphosphate accumulating organisms and denitrifying glycogen accumulating organisms[J]. Bioresource Technology, 2016, 219: 106-113.
69	WANG X, WANG S, XUE T, et al. Treating low carbon/nitrogen (C/N) wastewater in simultaneous nitrification-endogenous denitrification and phosphorous removal (SNDPR) systems by strengthening anaerobic intracellular carbon storage[J]. Water Research, 2015, 77: 191-200.
70	WANG X, WANG S, ZHAO J, et al. Combining simultaneous nitrification-endogenous denitrification and phosphorus removal with post-denitrification for low carbon/nitrogen wastewater treatment[J]. Bioresource Technology, 2016, 220: 17-25.
71	WANG X, WANG S, ZHAO J, et al. A novel stoichiometries methodology to quantify functional microorganisms in simultaneous (partial) nitrification-endogenous denitrification and phosphorus removal (SNEDPR)[J]. Water Research, 2016, 95: 319-329.
72	WANG X, ZHAO J, YU D, et al. Stable nitrite accumulation and phosphorous removal from nitrate and municipal wastewaters in a combined process of endogenous partial denitrification and denitrifying phosphorus removal (EPDPR)[J]. Chemical Engineering Journal, 2019,355: 560-571.
73	FAN Z, ZENG W, WANG B, et al. Microbial community at transcription level in the synergy of GAOs and Candidatus accumulibacter for saving carbon source in wastewater treatment[J]. Bioresource Technology, 2020, 297: 122454.
74	JI J, PENG Y, WANG B, et al.A novel SNPR process for advanced nitrogen and phosphorus removal from mainstream wastewater based on anammox, endogenous partial-denitrification and denitrifying dephosphatation[J]. Water Research, 2020, 170: 115363.
75	WANG X, ZHAO J, YU D, et al. Evaluating the potential for sustaining mainstream anammox by endogenous partial denitrification and phosphorus removal for energy-efficient wastewater treatment[J]. Bioresource Technology, 2019, 284: 302-314.
76	XU X, QIU L, WANG C, et al. Achieving mainstream nitrogen and phosphorus removal through simultaneous partial nitrification, anammox, denitrification, and denitrifying phosphorus removal (SNADPR) process in a single-tank integrative reactor[J]. Bioresource Technology, 2019, 284: 80-89.
77	WEN X, ZHOU J, LI Y, et al. A novel process combining simultaneous partial nitrification, anammox and denitrification (SNAD) with denitrifying phosphorus removal (DPR) to treat sewage[J]. Bioresource Technology, 2016, 222: 309-316.
78	ZHAO J, WANG X, LI X, et al. Advanced nutrient removal from ammonia and domestic wastewaters by a novel process based on simultaneous partial nitrification-anammox and modified denitrifying phosphorus removal[J]. Chemical Engineering Journal, 2018, 354: 589-598.
79	STROUS M, HEIJNEN J J, KUENEN J G, et al. The sequencing batch reactor as a powerful tool for the study of slowly growing anaerobic ammonium-oxidizing microorganisms[J]. Applied Microbiology and Biotechnology, 1998, 50(5): 589-596.
80	LOTTI T, KLEEREBEZEM R, HU Z, et al. Simultaneous partial nitritation and anammox at low temperature with granular sludge[J]. Water Research, 2014, 66: 111-121.
81	CHEN H, LIU S, YANG F, et al. The development of simultaneous partial nitrification, anammox and denitrification (SNAD) process in a single reactor for nitrogen removal[J]. Bioresource Technology, 2009, 100(4): 1548-1554.
82	WANG C, LIU S, XU X, et al. Achieving mainstream nitrogen removal through simultaneous partial nitrification, anammox and denitrification process in an integrated fixed film activated sludge reactor[J]. Chemosphere, 2018, 203: 457-466.
83	MIAO Y, ZHANG L, LI B, et al. Enhancing ammonium oxidizing bacteria activity was key to single-stage partial nitrification-anammox system treating low-strength sewage under intermittent aeration condition[J]. Bioresource Technology, 2017, 231: 36-44.
84	JI J, PENG Y, MAI W,et al. Achieving advanced nitrogen removal from low C/N wastewater by combining endogenous partial denitrification with anammox in mainstream treatment[J]. Bioresource Technology, 2018, 270: 570-579.
85	JI J, PENG Y, WANG B, et al. Achievement of high nitrite accumulation via endogenous partial denitrification (EPD)[J]. Bioresource Technology, 2017, 224: 140-146.
86	张立成. 亚硝化反硝化除磷工艺及分子微生物学研究[D].北京：北京工业大学, 2011.
86	ZHANG L C.Study on the nitrosation denitrifying phosphorus removal process and molecular microbiology[D].Beijing: Beijing University of Technology, 2011.
87	於蒙，潘婷，张淼，等. 乙酸钠丙酸钠配比对A²/O-BCO反硝化除磷及菌群结构的影响[J]. 中国环境科学, 2019, 39(10): 4178-4185.
87	YU M, PAN T, ZHANG M, et al.Effect of the ratios of sodium acetate to sodium propionate on denitrifying phosphorus removal characteristics in theA²/O-BCO process[J].China Environmental Science, 2019, 39(10): 4178-4185.

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