碳量子点/Fe3+复合材料用于生物传感检测抗坏血酸

doi:10.16085/j.issn.1000-6613.2018-2204

化工进展 ›› 2019, Vol. 38 ›› Issue (07): 3326-3331.DOI: 10.16085/j.issn.1000-6613.2018-2204

碳量子点/Fe³⁺复合材料用于生物传感检测抗坏血酸

高学霞¹(),周希^1,²(),刘美红¹,马玉峰²,王基夫^1,²,王春鹏^1,²(),储富祥^1,²

1. 中国林业科学研究院林产化学工业研究所，生物质化学利用国家工程实验室，国家林业局林产化学工程重点开放性实验室，江苏省生物质能源与材料重点实验室，江苏南京 210042
2. 南京林业大学林业资源高效加工利用协同创新中心，江苏南京 210042

收稿日期:2018-11-09 出版日期:2019-07-05 发布日期:2019-07-05
通讯作者: 周希,王春鹏
作者简介:高学霞（1992—），女，硕士研究生，研究方向为高分子材料。E-mail：<email>471821017@qq.com</email>。
基金资助:
中央级公益性科研院所基本科研业务费专项(CAFYBB2017ZX003)

Carbon dots/Fe³⁺ composites for biosensor detection of ascorbic acid

Xuexia GAO¹(),Xi ZHOU^1,²(),Meihong LIU¹,Yufeng MA²,Jifu WANG^1,²,Chunpeng WANG^1,²(),Fuxiang CHU^1,²

1. Institute of Chemical Industryof Forest Products, CAF, National Engineering Lab. for Biomass Chemical Utilization, Key and Open Lab. of Forest Chemical Engineering, SFA, Key Lab. of Biomass Energy and Material, Jiangsu Province, Nanjing 210042, Jiangsu, China
2. Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210042, Jiangsu, China

Received:2018-11-09 Online:2019-07-05 Published:2019-07-05
Contact: Xi ZHOU,Chunpeng WANG

摘要/Abstract

摘要：

利用碱木质素为原料，在浓硫酸与浓硝酸环境中，先进行超声处理，随后转移至反应釜中，180℃水热反应12h，制备碳量子点（CDs），并采用多种测试手段对其结构及形貌进行了表征。通过CDs溶液与Fe³⁺溶液相互作用，制备得到CDs/Fe³⁺复合材料。利用紫外光谱及荧光光谱研究了CDs及CDs/Fe³⁺复合材料的光学性能，发现CDs具有优异的荧光性能，其荧光量子产率达17.3%（硫酸喹啉为参比物质），而CDs/Fe³⁺复合材料荧光猝灭明显。将CDs/Fe³⁺复合材料应用于人体内不同生物活性分子检测，发现CDs/Fe³⁺复合材料对抗坏血酸（AA）具有特异性识别，且在0~200μmol/L及200~350μmol/L范围内，AA浓度与CDs/Fe³⁺复合材料的荧光强度呈现良好的线性关系，同时具有优异的选择识别性，可应用于生物体内AA的检测，在生物传感方面具有潜在的应用价值。

关键词: 碳量子点, 复合材料, 抗坏血酸, 生物传感器

Abstract:

Alkali lignin was used as raw material to prepare carbon dots (CDs) by ultrasonic treatment in concentrated sulfuric acid and nitric acid environment,and transferred to a reactor for hydrothermal reaction at 180℃ for 12h. The structure and morphology of CDs were characterized by various testing methods. CDs/Fe³⁺ composites were prepared by the interaction between CDs solution and Fe³⁺ solution. The optical properties of CDs and CDs/Fe³⁺ composites were studied by ultraviolet and fluorescence spectra. It was found that CDs had excellent fluorescence properties, and the fluorescence quantum yield of CDs was 17.3% (quinoline sulfate was the reference material), while the fluorescence quenching of CDs/Fe³⁺ composites was obvious. CDs/Fe³⁺ composite materials were used to detect different bioactive molecules in human body. It was found that CDs/Fe³⁺ composite materials could specifically recognize ascorbic acid (AA). In the range of 0―200 μmol/L and 200―350 μmol/L, the concentration of AA showed a good linear relationship with the fluorescence intensity of CDs/Fe³⁺ composite materials. It could be used in the detection of AA and has potential application in biosensing.

Key words: carbon dots, composite materials, ascorbic acid, biosensor

中图分类号:

TQ35

高学霞, 周希, 刘美红, 马玉峰, 王基夫, 王春鹏, 储富祥. 碳量子点/Fe³⁺复合材料用于生物传感检测抗坏血酸[J]. 化工进展, 2019, 38(07): 3326-3331.

Xuexia GAO, Xi ZHOU, Meihong LIU, Yufeng MA, Jifu WANG, Chunpeng WANG, Fuxiang CHU. Carbon dots/Fe³⁺ composites for biosensor detection of ascorbic acid[J]. Chemical Industry and Engineering Progress, 2019, 38(07): 3326-3331.

图/表 10

图1 CDs的XRPES谱图

图2 CDs的TEM图

图3 CDs及CDs/Fe3+的FTIR谱图

图4 CDs及CDs/Fe3+复合材料的紫外吸收光谱图

图5 CDs的荧光激发光谱及CDs与CDs/Fe3+复合材料的荧光发射光谱图

图6 CDs/Fe3+复合材料荧光强度受pH影响

图7 CDs/Fe3+复合材料对不同生物分子的荧光响应

图8 AA浓度对CDs/Fe3+复合材料荧光强度的影响

图9 AA浓度与CDs/Fe3+复合材料荧光强度的线性关系

图10 加AA前后的CDs/Fe3+溶液中加K3[Fe(CN)6]后颜色变化

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碳量子点/Fe³⁺复合材料用于生物传感检测抗坏血酸

Carbon dots/Fe³⁺ composites for biosensor detection of ascorbic acid

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