关键词: 载药, 仿生支架, 血液相容性, 细胞相容性, 缓释

Abstract:

The biomimetic structure of scaffolds alone has certain limitations on regulating cell behavior and bone tissue regeneration, so the combination of biomimetic scaffolds and nano-drug delivery has become an effective solution. A serial of 3D porous PLA/CS/GO/ASA biomimetic composite scaffolds with different ASA contents were successfully prepared by phase separation method. The addition of ASA destroyed the formation of PLA spherulite structure but had little effect on the structure of biomimetic micro-nano fiber. With the increasing of ASA, the hydrophilicity of drug-loaded scaffolds was improved in the experimental range, but the porosity showed a trend of decreasing first and then increasing, all of which were more than 80%. The hemolysis rate and platelet adhesion experiments showed that the scaffold material with good blood compatibility could be obtained by controlling the ASA content below 5%. Thecell proliferation experiments in vitro showed that the prepared drug-loaded scaffolds had cytocompatibility. Low ASA content could promote the proliferation of MC3T3-E1 cells, and high ASA content had a certain inhibitory effect on MC3T3-E1 cells. The sustained release experiments showed that the PLA/CS/GO/ASA drug-loading biomimetic composite scaffold had good sustained release performance.

Key words: drug-loaded, biomimetic scaffolds, hemocompatibility, cytocompatibility, sustained release