花菁类近红外荧光探针在生物检测中的应用研究进展

doi:10.16085/j.issn.1000-6613.2019-0442

摘要/Abstract

摘要：

随着现代生物学和生物技术的发展，近红外荧光探针作为一种重要的技术手段在许多领域都展现出了重要的应用价值。因具有背景干扰低、细胞损伤小、样品穿透性强、检测灵敏度高等优点，经常用于分子识别、医学诊断、生物分子检测以及生物成像等方面。本综述从金属离子检测、含硫小分子检测、活性氧（ROS）/活性氮（RNS）检测、酶识别、肿瘤细胞识别及治疗以及细胞内pH响应等方面，介绍了近年来花菁类荧光探针在生物检测中的应用研究进展。同时指出了花菁类荧光探针亟待解决的问题，通过花菁母体的结构修饰和改造提高探针的光稳定性、灵敏度、靶向性和水溶性，有望使其在生物检测以及疾病的诊断方面得到进一步应用发展。

关键词: 花菁, 近红外, 荧光探针, 生物检测

Abstract:

In modern biology and biotechnology, near-infrared fluorescence probes are of great value and importance in many fields as a powerful technological approach. They are frequently utilized in molecular recognition, medical diagnosis, biomolecular detection, and biological imaging, etc., due to their low auto-fluorescence inference, deep tissue penetration, high sensitivity, and negligible photo-damage to biological samples. In this article, the recent advance in cyanine-based near-infrared fluorescent probe for was briefly reviewed biological detection, such as metal ions, sulfur-containing small molecule, ROS/RNS, enzyme, tumor cell and intracellular pH. Although there were many problems need to be solved about cyanine-based probes, they were expected to be developed in biological detection and diagnosis of diseases with the improvement photostability, sensitivity, targeting and water-soluble by structural modifications.

Key words: cyanine, near-infrared, fluorescence probes, biological detection

中图分类号:

O625

于雪,包青青,姜超,张思霞,张丰旗,陈驰,陶娅妮,张跃伟. 花菁类近红外荧光探针在生物检测中的应用研究进展[J]. 化工进展, 2019, 38(12): 5492-5503.

Xue YU,Qingqing BAO,Chao JIANG,Sixia ZHANG,Fengqi ZHANG,Chi CHEN,Yani TAO,Yuewei ZHANG. Applied research progress on cyanine-based near-infrared fluorescent probe in biological detection[J]. Chemical Industry and Engineering Progress, 2019, 38(12): 5492-5503.

图/表 25

图1 荧光分子探针结构

图2 以花菁荧光团为基础的近红外荧光探针

图3 CAM探针的结构和CAM对Fe3+的检测机理

图4 用于检测Cu+的荧光探针结构

图5 Cy-Cu探针的结构和Cy-Cu对Cu2+的检测机理

图6 IR806-DPAP与Cu2+的结合模式及硫化物阴离子的取代机理

图7 CTMPA探针的结构和CTMPA对Zn2+的检测机理

图8 用于检测Hg2+的荧光探针结构

图9 Cy-NO2探针的结构和Cy-NO2对H2S的检测机理

图10 CyAC探针的结构和CyAC对Cys的检测机理

图11 Cy-NTe探针的结构和Cy-NTe对ONOO-和GSH的检测机理

图12 探针1和2的结构和探针1和2对ONOO-和GSH的检测机理

图13 Trp-Cy探针的结构和Trp-Cy对O3的检测机理

图14 Cy-FPA探针的结构和Cy-FPA对HClO的检测机理

图15 Cy-SN探针的结构和Cy-SN对ONOO-的检测机理

图16 CCy-OH探针的结构和CCy-OH对·OH的检测机理

图17 SeCy7探针的结构和SeCy7对NaClO的检测机理

图18 QcyP探针的结构和QcyP对ALP的检测机理

图19 近红外荧光探针的结构和探针对NTR的检测机理

图20 近红外荧光探针IR-780的结构

图21 近红外荧光探针IR-783和MHI-148的结构

图22 近红外荧光探针IR825-Cl的结构

图23 Tpy-Cy探针的结构和Tpy-Cy对细胞内pH的检测机理

图24 荧光探针的结构和荧光探针对细胞内pH的检测机理

图25 用于检测细胞内pH的荧光探针结构

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