静电纺丝制备蛋清蛋白/聚氧化乙烯纳米纤维

doi:10.16085/j.issn.1000-6613.2019-0557

摘要/Abstract

摘要：

本研究采用去离子水为溶剂，以蛋清蛋白与聚氧化乙烯（PEO）混合进行静电纺丝制备纳米纤维，采用扫描电镜表征了蛋清蛋白/PEO纳米纤维的形貌特征，探究了溶液质量分数以及纺丝工艺参数对蛋清蛋白/PEO纤维形貌的影响，并采用元素分析测试表征了纤维的元素组成成分。实验结果表明，蛋清在质量分数20%~80%之间表现出可纺性，蛋清质量分数50%的纺丝溶液进行纺丝，在纺丝电压25kV、纺丝距离16cm、挤出速度0.2mL/h的条件下，可纺性最好，制备的纤维无珠串平均直径为389nm，且纳米纤维中11.02%为氮元素，说明蛋清中的蛋白质成功转化为了纳米纤维。蛋清蛋白具有生物友好、可降解、来源广泛等优点，本研究成功实现了蛋清蛋白纳米纤维绿色制造，为其在生物医药、电池催化等领域的应用提供了基础。

关键词: 静电纺丝, 纳米纤维, 蛋白质, 工艺参数, 聚氧化乙烯

Abstract:

In this study, egg albumin and polyethylene oxide (PEO) were mixed to prepare nanofibers by electrospinning using deionized water as solvent. The morphology of egg albumin/PEO nanofibers was characterized by scanning electron microscopy (SEM). The effects of solution mass fraction and spinning parameters on the morphology of egg albumin/PEO fibers were investigated. The elemental composition of the fibers was characterized by elemental analysis. The results showed that the spinnability of the fibers was the best under the conditions of 25kV spinning voltage, 16cm spinning distance and 0.2mL/h extrusion speed. The fibers with an average diameter of 389nm were prepared and 11.02% of the nanofibers were nitrogen, which indicated that the protein in the egg white was successfully converted into nanofibers. Egg albumin had the advantages of bio-friendliness, biodegradability and wide sources. The successful realization of green manufacturing of egg albumin nanofibers provided a basis for its application in biomedicine, battery catalysis and other fields.

Key words: electrospinning, nanofibers, protein, process parameters, polyethylene oxide

中图分类号:

TQ34

吕婷婷,安瑛,刘宇健,李好义,谭晶,杨卫民. 静电纺丝制备蛋清蛋白/聚氧化乙烯纳米纤维[J]. 化工进展, 2019, 38(12): 5487-5491.

Tingting LÜ,Ying AN,Yujian LIU,Haoyi LI,Jing TAN,Weimin YANG. Preparation of egg white protein/polyethylene oxide nanofibers by electrospinning[J]. Chemical Industry and Engineering Progress, 2019, 38(12): 5487-5491.

图/表 7

图1 电纺蛋清蛋白/聚氧化乙烯纳米纤维装置图及纤维SEM图

表1 质量分数为20%～80%的溶液制得纤维的平均直径与直径标准差

溶液质量分数/%	平均直径/nm	直径标准差/nm
20	840	148
30	630	108
40	573	179
50	391	37
60	242	37
70	126	24
80	56	15

图2 纺丝电压对纤维平均直径的影响

图3 纺丝距离对纤维平均直径的影响

图4 挤出速度对纤维平均直径的影响

表2 蛋清蛋白纳米纤维中的元素组成

元素种类	所占比例/%
C	45.83
H	7.20
N	11.02
S	1.01

图5 鸡蛋的成分组成

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