化工进展 ›› 2021, Vol. 40 ›› Issue (6): 3346-3362.DOI: 10.16085/j.issn.1000-6613.2020-1297

• 材料科学与技术 • 上一篇    下一篇

反应型HClO/ClO-荧光探针的研究进展

安宁(), 高云玲()   

  1. 浙江工业大学化学工程学院,浙江 杭州 310014
  • 收稿日期:2020-07-09 修回日期:2020-08-27 出版日期:2021-06-06 发布日期:2021-06-22
  • 通讯作者: 高云玲
  • 作者简介:安宁(1995—),男,硕士研究生,研究方向为分子探针。E-mail:3022097952@qq.com
  • 基金资助:
    国家自然科学基金(21777143)

Reaction-based fluorescent probes for detection of HClO/ClO-

AN Ning(), GAO Yunling()   

  1. College of Chemical Engineering, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, China
  • Received:2020-07-09 Revised:2020-08-27 Online:2021-06-06 Published:2021-06-22
  • Contact: GAO Yunling

摘要:

次氯酸/次氯酸根是活性氧化物中重要的信号分子,在生物医药和环境安全等方面发挥着重要作用。反应型荧光探针基于出色的灵敏度、实时成像和生物兼容性好等特点,广泛应用于HClO/ClO-的检测。本文评述了近5年反应型HClO/ClO-荧光探针的研究进展,包括反应型HClO/ClO-荧光探针的设计策略和识别机理,并从HClO/ClO-引发的反应机理角度(碳碳双键的反应、硫族化合物的反应、醛肟基团的反应、腙/席夫碱的反应、酰肼/磺酰肼的反应、N,N-二甲基硫代氨基甲酸酯的反应和苯硼酸/硼酸酯的反应)概述了反应型HClO/ClO-荧光探针的特点和实际应用,指出反应型HClO/ClO-荧光探针的发展方向是合成性能优异的识别基团,构建选择性好、水溶性好、低荧光背景、光化学性能稳定和生物毒性低的反应型近红外HClO/ClO-荧光探针,实现对生物体内外HClO/ClO-的可视化检测及机理探索。

关键词: 次氯酸根, 荧光探针, 检测, 应用, 可视化

Abstract:

As one of the most vital reactive oxygen species, hypochlorous acid (HClO)/hypochlorite (ClO-) plays an indispensable role in biology, medicine and environmental safety. Nowadays, a growing number of reaction-based fluorescent probes have been successfully developed for detecting and imaging HClO/ClO- with excellent sensitivity, real time image and superior biocompatibility. In this paper, the research process of reaction-based fluorescent probes for HClO/ClO- was reviewed in the last five years, including design strategies, recognition mechanisms and the characteristics and practical application of reaction-based HClO/ClO- fluorescent probes based on the hypochlorous acid/hypochlorite-triggered response mechanisms including reaction of the C=C double bond, chalcogenide, aldoxime group, hydrazine/Schiff, acylhydrazine/sulfonylhydrazine, N,N-dimethylthiocarbamate and phenylo boric acid/boronic acid ester. In the future, it is desired to develop novel detection groups, explore recognition mechanisms, synthetize novel NIR reaction-based fluorescent probes with well selectivity, excellent water solubility, low background fluorescence, stable photochemical property and low biological toxicity, so as to visualize and detect HClO/ClO-in vitro and in vivo.

Key words: hypochlorite, fluorescent probe, detecting, application, visualize

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