盐酸-乙二醇-水介质高效预处理山核桃壳及其糖化工艺

doi:10.16085/j.issn.1000-6613.2015.08.045

化工进展 ›› 2015, Vol. 34 ›› Issue (08): 3188-3193,3206.DOI: 10.16085/j.issn.1000-6613.2015.08.045

盐酸-乙二醇-水介质高效预处理山核桃壳及其糖化工艺

张守雷, 何玉财, 纪俊玲, 陈群

常州大学石油化工学院, 江苏常州 213164

收稿日期:2014-12-24 修回日期:2015-01-24 出版日期:2015-08-05 发布日期:2015-08-05
通讯作者: 纪俊玲,博士,教授。E-mail jijunling@vip.163.com。
作者简介:张守雷(1988—),男,硕士研究生。
基金资助:
国家自然科学基金(21102011)、江苏省自然科学基金(BK20141172)及江苏省科技支撑计划(BE2014622)项目。

Effective pretreatment of hickory shell with hydrochloric acid-ethylene glycol-water media and its saccharification

ZHANG Shoulei, HE Yucai, JI Junling, CHEN Qun

School of Petrochemical Engineering, Changzhou University, Changzhou 213164, Jiangsu, China

Received:2014-12-24 Revised:2015-01-24 Online:2015-08-05 Published:2015-08-05

摘要/Abstract

摘要： 为了提高木质纤维素的酶解效率,采用盐酸辅助乙二醇对山核桃壳进行预处理。通过油浴的处理方式优化得出的最佳预处理条件为:处理介质为盐酸-乙二醇-水(1.2%:88.8%:10%,质量分数)的混合物,预处理温度为130℃,预处理时间为30min。为了减小预处理的温度和时间,采用微波辐射的辅助预处理,最佳预处理条件为:微波辐射温度100℃,微波辐射时间5min,微波辐射功率200W。糖化预处理后的山核桃壳经水解72h后,其还原糖产率可达到88.6%(油浴)和74.2%(微波)。利用电镜(SEM)和红外光谱(FT-IR)分析油浴和微波预处理后的山核桃壳,可以发现山核桃壳紧密的结构遭到破坏,变成更加易于酶解的松散、多孔结构,增加了酶可及度,因此很大程度上提高了糖化率。可见,盐酸-乙二醇-水溶液高效预处理可以提高山核桃壳酶解糖化的效率。

关键词: 山核桃壳, 乙二醇, 盐酸, 预处理, 优化, 酶解

Abstract: To improve the enzymatic saccharification of lignocellulosic biomass, ethylene glycol assisted with hydrochloric acid was used for pretreating hickory shell in this study. After the optimization in oil-bath system, the optimal pretreatment condition was obtained as following:the pretreatment media was hydrochloric acid-ethylene glycol-water (1.2%:88.8%:10%, mass ratio), pretreatment temperature 130℃, and pretreatment time 30min. In order to reduce the pretreatment temperature and time, microwave radiation was employed to assist the pretreatment with hydrochloric acid-ethylene glycol-water (1.2%:88.8%:10%, mass ratio) media, and the optimal pretreatment condition was achieved as following:microwave radiation temperature 100℃, microwave radiation time 5min, and microwave radiation power 200W. These results showed that the saccharification rate of pretreated hickory shell was higher than that of untreated one. After the pretreatment with oil-bath and microwave radiation, the reducing sugar yield from the enzymatic hydrolysis could reach 88.6% (oil bath) and 74.2% (microwave) after 72h. By using electron microscopy (SEM) and infrared spectroscopy (FT-IR) analysis, the structure of pretreated hickory shell was destroyed, the structure became loose, and porous were easier to be enzymatically hydrolyzed, which increased the enzyme accessibility, thereby greatly improving the saccharification rate. Therefore, the pretreatment with hydrochloric acid-ethylene glycol-water solution can improve the efficiency of the enzymatic saccharification of hickory shell.

Key words: hickory shell, ethylene glycol, hydrochloride acid, pretreatment, optimization, enzymatic hydrolysis

中图分类号:

Q93
X703

张守雷, 何玉财, 纪俊玲, 陈群. 盐酸-乙二醇-水介质高效预处理山核桃壳及其糖化工艺[J]. 化工进展, 2015, 34(08): 3188-3193,3206.

ZHANG Shoulei, HE Yucai, JI Junling, CHEN Qun. Effective pretreatment of hickory shell with hydrochloric acid-ethylene glycol-water media and its saccharification[J]. Chemical Industry and Engineering Progree, 2015, 34(08): 3188-3193,3206.

参考文献

[1] Bi D, Chu D, Zhu P, et al. Utilization of dry distiller's grain and solubles as nutrient supplement in the simultaneous saccharification and ethanol fermentation at high solids loading of corn stover[J]. Biotechnology Letters, 2011, 33(2): 273-276.
[2] Imman S, Arnthong J, Burapatana V, et al. Autohydrolysis of tropical agricultural residues by compressed liquid hot water pretreatment[J]. Applied Biochemistry and Biotechnology, 2013, 170(8): 1982-1995.
[3] Adsul M G, Singhvi M S, Gaikaiwari S A, et al. Development of biocatalysts for production of commodity chemicals from lignocellulosic biomass[J]. Bioresource Technology, 2011, 102(6): 4304-4312.
[4] Dong H, Bao J. Metabolism: Biofuel via biodetoxification[J]. Nature Chemical Biology, 2010, 6(5): 316-318.
[5] Qing Q, Hu R, He Y C, et al. Investigation of a novel acid-catalyzed ionic liquid pretreatment method to improve biomass enzymatic hydrolysis conversion[J]. Applied Microbiology and Biotechnology, 2014, 98(11): 5275-5286.
[6] Chu D, Zhang J, Bao J. Simultaneous saccharification and ethanol fermentation of corn stover at high temperature and high solids loading by a thermotolerant strain Saccharomyces cerevisiae DQ1[J]. BioEnergy Research, 2012, 5(4): 1020-1026.
[7] Jing X, Zhang X, Bao J. Inhibition performance of lignocellulose degradation products on industrial cellulase enzymes during cellulose hydrolysis[J]. Applied Biochemistry and Biotechnology, 2009, 159(3): 696-707.
[8] 夏东琴, 何玉财, 谭春伟, 等. 一株耐NMMO纤维素酶菌株的筛化[J]. 化工进展, 2013, 32(2): 420-424.
[9] Fu D, Mazza G. Optimization of processing conditions for the pretreatment of wheat straw using aqueous ionic liquid[J]. Bioresource Technology, 2011, 102(17): 8003-8010.
[10] Leschine S B. Cellulose degradation in anaerobic environments[J]. Annual Reviews in Microbiology, 1995, 49(1): 399-426.
[11] Gerez J C C, Gerez M D C A, Miller J. Process and installation for obtaining ethanol by the continuous acid hydrolysis of cellulosic materials: US, 4529699[P]. 1985-7-16.
[12] 王景全, 赵红, 胡湛波, 等. 甘蔗渣高沸醇预处理过程中木质素和碳水化合物的溶出规律[J]. 中国造纸, 2011, 30(5): 21-25.
[13] Tai C, Keshwani D. Impact of pretreatment with dilute sulfuric acid under moderate temperature on hydrolysis of corn stover with two enzyme systems[J]. Applied Biochemistry and Biotechnology, 2014, 172(5): 2628-2639.
[14] Farone W, Cuzens J E. Strong acid hydrolysis of cellulosic and hemicellulosic materials[J]. Biotechnology Advances, 1997, 15(3): 798-798.
[15] Arifin Z B, Teoh T C. Conversion of cellulosic materials into glucose for use in bioethanol production: US, 13/146, 207[P]. 2010-1-29.
[16] He Y C, Gong L, Liu F, et al. Waste biogas residue from cassava dregs as carbon source to produce Galactomyces sp. Cczu11-1 cellulase and its enzymatic saccharification[J]. Applied Biochemistry and Biotechnology, 2014, 173(4): 894-903.
[17] He Y C, Xia D Q, Ma C L, et al. Enzymatic saccharification of sugarcane bagasse by N-methylmorpholine-N-oxide-tolerant cellulose from a newly isolated Galactomyces sp. CCZU11-1[J]. Bioresource Technology, 2013, 135: 18-22.
[18] Zhang Z, O'Hara I M, Doherty W O S. Pretreatment of sugarcane bagasse by acid-catalysed process in aqueous ionic liquid solutions[J]. Bioresource Technology, 2012, 120: 149-156.
[19] 刘传富, 张爱萍, 李维英, 等. 纤维素在新型绿色溶剂离子液体中的溶解及其应用[J]. 化学进展, 2009, 21(9): 1800-1806.
[20] 李志强, 江泽慧, 费本华, 等. 酸和碱预处理对麻竹酶水解糖化的影响[J]. 化工进展, 2012, 31(s1): 60-63.
[21] 孙孟林, 胡国新, 赵宇, 等. 离子液体 [BMIM] Cl-水体系中纤维素制氢试验研究[J]. 可再生能源, 2013, 31(6): 70-74.
[22] Zhang Y H P, Cui J, Lynd L R, et al. A transition from cellulose swelling to cellulose dissolution by o-phosphoric acid: Evidence from enzymatic hydrolysis and supramolecular structure[J]. Biomacromolecules, 2006, 7(2): 644-648.
[23] 龚磊, 吴殷琦, 何玉财, 等. 稀酸辅助离子液体1,3-二甲基咪唑磷酸二甲酯高效预处理木薯渣厌氧发酵残渣[J]. 化工进展, 2014, 33(6): 1567-1571.
[24] Li H, Xu J. Optimization of microwave-assisted calcium chloride pretreatment of corn stover[J]. Bioresource Technology, 2013, 127: 112-118.
[25] Jackowiak D, Bassard D, Pauss A, et al. Optimisation of a microwave pretreatment of wheat straw for methane production[J]. Bioresource Technology, 2011, 102(12): 6750-6756.
[26] Xu J, Chen H, Kádár Z, et al. Optimization of microwave pretreatment on wheat straw for ethanol production[J]. Biomass and Bioenergy, 2011, 35(9): 3859-3864.
[27] 贾艳宗, 马沛生, 王彦飞. 微波在酯化和水解反应中的应用[J]. 化工进展, 2004, 23(6): 641-644.

盐酸-乙二醇-水介质高效预处理山核桃壳及其糖化工艺

Effective pretreatment of hickory shell with hydrochloric acid-ethylene glycol-water media and its saccharification

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	李梦圆, 郭凡, 李群生. 聚乙烯醇生产中回收工段第三、第四精馏塔的模拟与优化[J]. 化工进展, 2023, 42(S1): 113-123.
[2]	张瑞杰, 刘志林, 王俊文, 张玮, 韩德求, 李婷, 邹雄. 水冷式复叠制冷系统的在线动态模拟与优化[J]. 化工进展, 2023, 42(S1): 124-132.
[3]	赵晨, 苗天泽, 张朝阳, 洪芳军, 汪大海. 负压状态窄缝通道乙二醇水溶液传热特性[J]. 化工进展, 2023, 42(S1): 148-157.
[4]	王福安. 300kt/a环氧丙烷工艺反应器降耗减排分析[J]. 化工进展, 2023, 42(S1): 213-218.
[5]	陈匡胤, 李蕊兰, 童杨, 沈建华. 质子交换膜燃料电池气体扩散层结构与设计研究进展[J]. 化工进展, 2023, 42(S1): 246-259.
[6]	王正坤, 黎四芳. 双子表面活性剂癸炔二醇的绿色合成[J]. 化工进展, 2023, 42(S1): 400-410.
[7]	汪鹏, 张洋, 范兵强, 何登波, 申长帅, 张贺东, 郑诗礼, 邹兴. 高碳铬铁盐酸浸出过程工艺及动力学[J]. 化工进展, 2023, 42(S1): 510-517.
[8]	孙玉玉, 蔡鑫磊, 汤吉海, 黄晶晶, 黄益平, 刘杰. 反应精馏合成甲基丙烯酸甲酯工艺优化及节能[J]. 化工进展, 2023, 42(S1): 56-63.
[9]	李春利, 韩晓光, 刘加朋, 王亚涛, 王晨希, 王洪海, 彭胜. 填料塔液体分布器的研究进展[J]. 化工进展, 2023, 42(9): 4479-4495.
[10]	刘炫麟, 王驿凯, 戴苏洲, 殷勇高. 热泵中氨基甲酸铵分解反应特性及反应器结构优化[J]. 化工进展, 2023, 42(9): 4522-4530.
[11]	王晨, 白浩良, 康雪. 大功率UV-LED散热与纳米TiO₂光催化酸性红26耦合系统性能[J]. 化工进展, 2023, 42(9): 4905-4916.
[12]	李由, 吴越, 钟禹, 林琦璇, 任俊莉. 酸性熔盐水合物预处理麦秆高效制备木糖及其对酶解效率的影响[J]. 化工进展, 2023, 42(9): 4974-4983.
[13]	李海东, 杨远坤, 郭姝姝, 汪本金, 岳婷婷, 傅开彬, 王哲, 何守琴, 姚俊, 谌书. 炭化与焙烧温度对植物基铁碳微电解材料去除As(Ⅲ)性能的影响[J]. 化工进展, 2023, 42(7): 3652-3663.
[14]	薛凯, 王帅, 马金鹏, 胡晓阳, 种道彤, 王进仕, 严俊杰. 工业园区分布式综合能源系统的规划与调度[J]. 化工进展, 2023, 42(7): 3510-3519.
[15]	周龙大, 赵立新, 徐保蕊, 张爽, 刘琳. 电场-旋流耦合强化多相介质分离研究进展[J]. 化工进展, 2023, 42(7): 3443-3456.