酸性熔盐水合物预处理麦秆高效制备木糖及其对酶解效率的影响

doi:10.16085/j.issn.1000-6613.2022-1955

摘要/Abstract

摘要：

秸秆预处理的目的是提高组分拆解以及酶解效率。本研究提出一种绿色温和、可循环使用的酸性熔盐水合物预处理农业秸秆的方法，能实现小麦秸秆中半纤维素的选择性拆解并定向解聚为木糖，同时保持较高的纤维素保留率。预处理体系以氯化锌熔盐水合物为溶剂协同酸作催化剂，研究了反应温度、反应时间与硫酸浓度对组分拆解效率的影响，最优反应条件为：温度97℃，时间19.0min，硫酸质量分数3.3%。在此条件下木糖得率达到90.85%，纤维素保留率为85.87%。将预处理后残渣经过酶水解72h后，葡萄糖酶解得率最高可达77.81%。此外，还研究了该体系中氯化锌的循环利用情况，5次循环利用后，在保持氯化锌固体较高回收率的情况下（质量分数为80%），预处理木糖得率达87.69%，依然表现出良好的预处理效果。

关键词: 麦秆, 熔盐水合物, 预处理, 木糖, 酶水解

Abstract:

Straw pretreatment is used to improve component fractionation as well as enzymatic efficiency. In this study, we proposed a green, mild and recyclable pretreatment method of acidic molten salt hydrate for agricultural straw, which could achieve selective hemicellulose fractionation and target depolymerization of hemicellulose to xylose while maintaining a high retention of cellulose. The optimal pretreatment conditions were 97℃, 19.0min and 3.3% sulfuric acid concentration, under this condition the xylose yield was 90.85% and the cellulose retention was 85.87%. After enzymatic hydrolysis of the pretreated residue for 72h, the glucose enzymatic yield was up to 77.81%. In addition, the recycling of ZnCl₂ in this system was also studied. After five cycles of recycling, the pretreatment xylose yield reached 87.69% with maintaining a high recovery of ZnCl₂ solids (80%), which was indicative of a good pretreatment effect.

Key words: straw, molten salt hydrate, pretreatment, xylose, enzyme hydrolysis

中图分类号:

O636.11

李由, 吴越, 钟禹, 林琦璇, 任俊莉. 酸性熔盐水合物预处理麦秆高效制备木糖及其对酶解效率的影响[J]. 化工进展, 2023, 42(9): 4974-4983.

LI You, WU Yue, ZHONG Yu, LIN Qixuan, REN Junli. Pretreatment of wheat straw with acidic molten salt hydrate for xylose production and its effect on enzymatic hydrolysis efficiency[J]. Chemical Industry and Engineering Progress, 2023, 42(9): 4974-4983.

图/表 12

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样品名称	固液比	质量收率 /%	残渣组成/%
样品名称	固液比	质量收率 /%	纤维素	半纤维素	木质素
脱蜡后原料	—	—	36.49	23.13	20.58
熔盐处理后残渣	1∶10	86.77	40.34	21.93	23.67
稀酸处理后残渣	1∶10	85.34	43.25	19.09	24.95
酸性熔盐处理后残渣	1∶5	73.94	52.26	6.81	27.97
	1∶10	68.26	53.27	3.24	33.94
	1∶15	67.82	53.91	3.12	34.61

样品名称	固液比	质量收率 /%	残渣组成/%
样品名称	固液比	质量收率 /%	纤维素	半纤维素	木质素
脱蜡后原料	—	—	36.49	23.13	20.58
熔盐处理后残渣	1∶10	86.77	40.34	21.93	23.67
稀酸处理后残渣	1∶10	85.34	43.25	19.09	24.95
酸性熔盐处理后残渣	1∶5	73.94	52.26	6.81	27.97
	1∶10	68.26	53.27	3.24	33.94
	1∶15	67.82	53.91	3.12	34.61

来源	总和	自由度	均方	F 值	P值	显著性
模型	1384.69	9	153.85	5.97	0.0140	显著
A	40.91	1	40.91	1.59	0.2482
B	735.94	1	735.94	28.55	0.0011
C	236.10	1	236.10	9.16	0.0192
AB	2.74	1	2.74	0.11	0.7540
AC	1.08	1	0.000	0.042	0.8435
BC	1.72	1	0.000	0.067	0.8038
A²	131.47	1	131.47	5.10	0.0585
B²	61.05	1	61.05	2.37	0.1677
C²	136.57	1	136.57	5.30	0.0549
剩余量	180.47	7	25.78
失拟	144.77	3	48.26	5.41	0.0683	不显著
纯误差	35.70	4	8.92
总和	1565.16	16

来源	总和	自由度	均方	F 值	P值	显著性
模型	1384.69	9	153.85	5.97	0.0140	显著
A	40.91	1	40.91	1.59	0.2482
B	735.94	1	735.94	28.55	0.0011
C	236.10	1	236.10	9.16	0.0192
AB	2.74	1	2.74	0.11	0.7540
AC	1.08	1	0.000	0.042	0.8435
BC	1.72	1	0.000	0.067	0.8038
A²	131.47	1	131.47	5.10	0.0585
B²	61.05	1	61.05	2.37	0.1677
C²	136.57	1	136.57	5.30	0.0549
剩余量	180.47	7	25.78
失拟	144.77	3	48.26	5.41	0.0683	不显著
纯误差	35.70	4	8.92
总和	1565.16	16

木糖得率/%		相对误差/%
实际值	预测值	相对误差/%
90.05	91.91	2.02
90.85	91.91	1.15
89.76	91.91	2.34
90.34	91.91	1.71
89.82	91.91	2.27