关键词: 左旋多巴, 全化学合成, 植物提取, 生物转化, 代谢工程, 左旋多巴, 全化学合成, 植物提取, 生物转化, 代谢工程

Abstract: L-dopa has been widely used in clinical treatment for Parkinson's disease. The methods for L-DOPA production，including total chemical synthesis，extraction from plants，enzyme bioconversion and metabolic engineering，were summarized in this review. The total chemical synthesis of L-dopa was complicate due to its asymmetric process and associated with certain environmental issues. Extracting L-dopa from plant suffered low yield and was limited by plant resource. Bioconversion of phenols to L-dopa has been catalyzed by enzymes tyrosine phenol lyase，tyrosinase and transaminase. It is suggested that with the development of synthetic biology，fermentation of L-dopa by constructing metabolically engineered microorganisms would be a new high efficient and environment-friendly method，which demonstrates a promising process.

Key words: L-dopa, total chemical synthesis, plant extraction, bioconversion, metabolic engineering, L-dopa, total chemical synthesis, plant extraction, bioconversion, metabolic engineering