化工进展 ›› 2018, Vol. 37 ›› Issue (11): 4427-4436.DOI: 10.16085/j.issn.1000-6613.2018-0134

• 生物与医药化工 • 上一篇    下一篇

生物法生产3-羟基丙酸研究进展

于新磊1,2,3, 毛雨丰1,2,3, 张晓霞1,2,3, 陆凌雪1,2,3, 王智文1,2,3, 陈涛1,2,3   

  1. 1 天津大学化工学院, 天津 300345;
    2 天津大学系统生物工程教育部重点实验室, 天津 300345;
    3 天津化学化工协同创新中心, 天津 300350
  • 收稿日期:2018-01-16 修回日期:2018-03-15 出版日期:2018-11-05 发布日期:2018-11-05
  • 通讯作者: 陈涛,教授,研究方向为微生物代谢工程与合成生物学。E-mail:chentao@tju.edu.cn。
  • 作者简介:于新磊(1992-),女,硕士研究生。
  • 基金资助:
    国家自然科学基金项目(21776208,21390201,21621004)。

Recent progress in microbial production of 3-hydroxypropionic acid

YU Xinlei1,2,3, MAO Yufeng1,2,3, ZHANG Xiaoxia1,2,3, LU Lingxue1,2,3, WANG Zhiwen1,2,3, CHEN Tao1,2,3   

  1. 1 School of Chemical Engineering and Technology, Tianjin University, Tianjin 300345, China;
    2 Key Laboratory of Systems Bioengineering(Ministry of Education), Tianjin University, Tianjin 300345, China;
    3 Collaborative Innovation Center of Chemical Science and Engineering(Tianjin), Tianjin 300350, China
  • Received:2018-01-16 Revised:2018-03-15 Online:2018-11-05 Published:2018-11-05

摘要: 3-羟基丙酸(3-hydroxypropionic acid,3-HP)是一种具有末端羟基与羧基双活性基团、应用广泛的有机酸,生物法合成3-HP具有高效、绿色、可持续发展等优势。本文分析了生物法生产3-HP的主要合成途径、主要生产菌种及菌株改造、发酵工艺优化3个方面的研究进展。重点介绍了以甘油合成途径、丙二酰辅酶A合成途径、β-丙氨酸合成途径为代表的主要合成途径及相应途径的关键酶;以克雷伯氏肺炎球菌、酵母菌及大肠杆菌为代表的主要生产菌株及其改造策略;包括分批发酵、两阶段发酵及固定化细胞催化等在内的发酵工艺优化策略3个方面的研究内容。最后指出了进一步筛选改造甘油脱水酶等途径的关键酶;基于先进基因组编辑技术开发的非致病性、3-HP高耐受型菌株,挖掘新的3-HP合成途径;开发更加经济、高效、环保的发酵工艺路线,将是今后生物法生产3-HP的研究重点。

关键词: 3-羟基丙酸, 微生物发酵, 代谢工程, 工程菌株, 生物合成途径, 甘油脱水酶

Abstract: 3-Hydroxypropionic acid (3-HP) is an organic acid with terminal hydroxy and carboxyl reactive groups and has wide applications in many industrial sectors. Biosynthesis of 3-HP is highly efficient, green and sustainable. In this review, the authors analyzed the major synthetic pathways of 3-HP bio-production, representative production hosts, strategies of strain development, and optimization of fermentation parameters. The review focused on the recent research progresses on the following aspects, including major 3-HP pathways such as glycerol pathway; malonyl-CoA pathway; β-alanine pathway and key enzymes of each pathway, representative 3-HP production hosts such as Klebsiella pneumoniae; Saccharomyces and Escherichia coli and strategies of strain development, optimization of 3-HP bio-production processes such as batch operation; two-stage fermentation and immobilized cell fermentation. In the end, the authors pointed out that screening of key enzymes like glycerol dehydratase, development of novo 3-HP synthetic pathways and non-pathogenic production host with high 3-HP tolerance via advanced genomic editing technique and development of more economic; highly efficient and environmental friendly bio-production process are vital research topics in the future.

Key words: 3-hydroxypropionic acid, microbial fermentation, metabolic engineering, engineering strain, biosynthetic pathways, glycerol dehydratase

中图分类号: 

京ICP备12046843号-2;京公网安备 11010102001994号
版权所有 © 《化工进展》编辑部
地址:北京市东城区青年湖南街13号 邮编:100011
电子信箱:hgjz@cip.com.cn
本系统由北京玛格泰克科技发展有限公司设计开发 技术支持:support@magtech.com.cn