餐厨垃圾生物减量菌种筛选及加菌方式优化

doi:10.16085/j.issn.1000-6613.2023-1974

摘要/Abstract

摘要：

餐厨垃圾中含水率与有机物含量高，若处置不当极易造成环境污染。生物减量技术能有效降低餐厨垃圾中水分与有机物，减轻后期处置压力，达到减量化的目的。为提高餐厨垃圾生物减量效率，通过研究菌株生长特性以及单菌餐厨垃圾生物减量实验，从实验室前期研究筛得的10株菌中筛选出4株高效降解餐厨垃圾菌制得复配菌剂，以生物强化的方式加入餐厨垃圾的自产热降解体系中，可使反应高温期提前1天到达，挥发性固体去除率（VSR）提高6.72%~25.82%。并通过分段加菌进一步加速了反应进程，VSR相较于一次性加菌提高了3.87%，研究表明分段加菌方式更适用于餐厨垃圾自产热降解反应。

关键词: 餐厨垃圾, 生物减量, 降解, 分段加菌, 优化

Abstract:

Due to high moisture and organic matter content, improper disposal of food waste can easily cause environmental pollution. Biodegradation can effectively reduce the moisture and organic matter in food waste, reduce the pressure of post-disposal, and achieve the purpose of minimization. In order to further improve the efficiency of food waste biodegradation, by studying the growth characteristics of bacteria and single-bacterial biodegradation experiment of food waste, 4 strains of highly efficient degrading bacteria were screened from the existing 10 in the laboratory to produce a compound bacterial agent, which was added into the autothermal degradation system of food waste through bio-augmentation, so that the high-temperature period could be reached 1d earlier, and the volatile solids removal rate was increased by 6.72%—25.82%. Moreover, the reaction process was further accelerated by staged inoculation of bacteria, with a 3.87% increase in VSR compared with one-time inoculation method, which proved that the staged inoculation of bacterial method was more suitable for the autothermal degradation of food waste.

Key words: food waste, biodegradation, degradation, staged inoculation of bacteria, optimization

中图分类号:

X705

邵毅恒, 杨宁, 赵玥, 李保国, 刘莉. 餐厨垃圾生物减量菌种筛选及加菌方式优化[J]. 化工进展, 2024, 43(12): 7018-7024.

SHAO Yiheng, YANG Ning, ZHAO Yue, LI Baoguo, LIU Li. Screening of food waste biodegradation bacteria and optimization of inoculation methods[J]. Chemical Industry and Engineering Progress, 2024, 43(12): 7018-7024.

图/表 10

参考文献 21

1	国家统计局. 中华人民共和国2022年国民经济和社会发展统计公报[J]. 中国统计, 2023(3): 12-29.
	National Bureau of Statistics. Statistical communique on national economic and social development of People’s republic of China (PRC) in 2022[J]. China Statistics, 2023(3): 12-29.
2	崔文静, 陆敏博. 餐厨垃圾处理现状及今后发展趋势[J]. 广东化工, 2021, 48(19): 140-141.
	CUI Wenjing, LU Minbo. The present disposing situation and development trend of kitchen waste[J]. Guangdong Chemical Industry, 2021, 48(19): 140-141.
3	辛梓弘. 餐厨垃圾处理技术研究[J]. 当代化工研究, 2022(9): 63-65.
	XIN Zihong. Study on food waste treatment technologies[J]. Modern Chemical Research, 2022(9): 63-65.
4	TRIGO Almudena, VALENCIA Alfonso, CASES Ildefonso. Systemic approaches to biodegradation[J]. FEMS Microbiology Reviews, 2009, 33(1): 98-108.
5	KE Xia, SUN Jiacheng, LIU Cong, et al. Fed-in-situ biological reduction treatment of food waste via high-temperature-resistant oil degrading microbial consortium[J]. Bioresource Technology, 2021, 340: 125635.
6	ZHOU Shipeng, KE Xia, ZHOU Haiyan, et al. Community scale in situ rapid biological reduction and resource recovery of food waste[J]. Bioresource Technology, 2022, 346: 126603.
7	PITERINA Anna V, BARTLETT John, TONY PEMBROKE J. Phylogenetic analysis of the bacterial community in a full scale autothermal thermophilic aerobic digester (ATAD) treating mixed domestic wastewater sludge for land spread[J]. Water Research, 2012, 46(8): 2488-2504.
8	ROBINSON T P, WIMPENNY J W, EARNSHAW R G. pH gradients through colonies of Bacillus cereus and the surrounding agar[J]. Journal of General Microbiology, 1991, 137(12): 2885-2889.
9	环境保护部. 水质总有机碳的测定燃烧氧化—非分散红外吸收法: [S]. 北京: 中国科学环境出版社, 2009.
	Ministry of Environmental Protection. Water quality—Determination of total organic carbon—Nondispersive infrared absorption method: [S]. Beijing: China Environmental Press, 2009.
10	国家卫生和计划生育委员会, 国家食品药品监督管理总局. 食品安全国家标准食品中蛋白质的测定: [S]. 北京: 中国标准出版社, 2017.
	National Health and Family Planning Commission, National State Food and Drug Administration. National food safety standard Determination of protein in foods: [S]. Beijing: Standards Press of China, 2017.
11	中华人民共和国国家卫生和计划生育委员会. 食品安全国家标准食品中水分的测定: [S]. 北京: 中国标准出版社, 2017.
	National Health and Family Planning Commission of the People’s Republic of China. National food safety standard determination of moisture in food: [S]. Beijing: Standards Press of China, 2017.
12	PAWŁOWSKI L. Standard methods for the examination of water and wastewater[M]. Washington, DC: American public health association, 2012.
13	崔静岚, 陈晨, 秦智慧, 等. 嗜热菌对有机污染物的降解及其应用研究进展[J]. 应用生态学报, 2012, 23(11): 3218-3226.
	CUI Jinglan, CHEN Chen, QIN Zhihui, et al. Biodegradation of organic pollutants by thermophiles and their applications: A review[J]. Chinese Journal of Applied Ecology, 2012, 23(11): 3218-3226.
14	郑宗明, 陈珍, 孙燕, 等. pH对克雷伯氏菌1,3-丙二醇合成关键酶酶活及发酵特性的影响[J]. 工业微生物, 2010, 40(3): 48-52.
	ZHENG Zongming, CHEN Zhen, SUN Yan, et al. Effects of pH on activities of key enzymes and 1,3-propanediol fermentation properties with Klebsiella pneumoniae [J]. Industrial Microbiology, 2010, 40(3): 48-52.
15	GE Mianshen, SHEN Yujun, DING Jingtao, et al. New insight into the impact of moisture content and pH on dissolved organic matter and microbial dynamics during cattle manure composting[J]. Bioresource Technology, 2022, 344(Pt A): 126236.
16	QUEVAUVILLER P. The EC Standards, Measurements and testing programme in support of the quality control of waste analysis[J]. Talanta, 1998, 46(3): 457-463.
17	TANG Ruolan, LIU Yan, MA Ruonan, et al. Effect of moisture content, aeration rate, and C/N on maturity and gaseous emissions during kitchen waste rapid composting[J]. Journal of Environmental Management, 2023, 326(Pt A): 116662.
18	刘英杰, 李琬婷, 王海候, 等.锯末添加量对餐厨废弃物生物干化效率和细菌群落的影响[J]. 农业工程学报, 2023, 39(15): 208-216.
	LIU Yingjie, LI Wanting, WANG Haihou, et al. Effects of sawdust addition on bio-drying efficiency and bacterial community of food waste[J]. Transactions of the Chinese Society of Agricultural Engineering, 2023, 39(15): 208-216.
19	王若斐, 刘超, 操一凡, 等. 不同碳氮比猪粪堆肥及其产品肥效[J]. 中国土壤与肥料, 2017(6): 127-134, 154.
	WANG Ruofei, LIU Chao, CAO Yifan, et al. Research on the composting of pig manure and rice husk with different C/N and plant growth promotion effects of the products[J]. Soil and Fertilizer Sciences in China, 2017(6): 127-134, 154.
20	ALAREFEE Hamed Ahmed, ISHAK Che Fauziah, OTHMAN Radziah, et al. Effectiveness of mixing poultry litter compost with rice husk biochar in mitigating ammonia volatilization and carbon dioxide emission[J]. Journal of Environmental Management, 2023, 329: 117051.
21	弓凤莲, 汪强, 李培培. 应用灰色关联度评价不同物料堆肥腐熟度的研究[J]. 安徽农业科学, 2014, 42(29): 10054-10056.
	GONG Fenglian, WANG Qiang, LI Peipei. Application of grey correlation degree of evaluation of different organic fertilizer rotten proportion[J]. Journal of Anhui Agricultural Sciences, 2014, 42(29): 10054-10056.

编辑推荐 0

Metrics

阅读次数

全文

HTML			PDF

最新录用	在线预览	正式出版	最新录用	在线预览	正式出版
0	0	3	0	0	21

来源	本网站	其他网站

次数	13	11
比例	54%	46%

摘要

最新录用	在线预览	正式出版

0	0	59

来源	本网站	其他网站

次数	11	48
比例	19%	81%

菌株编号	生长情况				最佳生长温度/℃	鉴定结果
菌株编号	30℃	40℃	50℃	60℃	最佳生长温度/℃	鉴定结果
N3-1	++	++	++	+	40~50	Geobacillus thermoparaffinivorans
N3-3	-	-	++	++	50~60	Geobacillus caldoxylosilyticus
4-2	+++	+++	++	-	30~40	Acinetobacter pittii
G4-1	++	++	+++	+++	50~60	Geobacillus thermoleovorans
G6-1	-	+++	++	-	40~50	Geobacillus lituanicus
C7	-	-	++	-	50	Bacillus smithii
BM1-2	+++	+++	++	-	30~40	Bacillus megaterium
BM1-4	++	+++	+++	-	40~50	Bacillus amyloliquefaciens
BM2-2	+++	+++	++	-	30~40	Bacillus velezensis
BM2-3	++	+++	+++	-	40~50	Bacillus licheniformis

菌株编号	生长情况				最佳生长温度/℃	鉴定结果
菌株编号	30℃	40℃	50℃	60℃	最佳生长温度/℃	鉴定结果
N3-1	++	++	++	+	40~50	Geobacillus thermoparaffinivorans
N3-3	-	-	++	++	50~60	Geobacillus caldoxylosilyticus
4-2	+++	+++	++	-	30~40	Acinetobacter pittii
G4-1	++	++	+++	+++	50~60	Geobacillus thermoleovorans
G6-1	-	+++	++	-	40~50	Geobacillus lituanicus
C7	-	-	++	-	50	Bacillus smithii
BM1-2	+++	+++	++	-	30~40	Bacillus megaterium
BM1-4	++	+++	+++	-	40~50	Bacillus amyloliquefaciens
BM2-2	+++	+++	++	-	30~40	Bacillus velezensis
BM2-3	++	+++	+++	-	40~50	Bacillus licheniformis

材料	理化性质
材料	含水率/%	总固体/%	挥发性固体/%	pH	TOC/g·(kg TS)^-1	TN/g·(kg TS)^-1
餐厨垃圾（FW）	72.95±0.24	27.04±0.24	92.10±0.13	5.17±0.13	499.30±1.12	31.51±0.37
木屑（SD）	11.73±0.21	88.27±0.21	95.82±0.22	6.42±0.06	462.80±1.53	2.31±0.03

材料	理化性质
材料	含水率/%	总固体/%	挥发性固体/%	pH	TOC/g·(kg TS)^-1	TN/g·(kg TS)^-1
餐厨垃圾（FW）	72.95±0.24	27.04±0.24	92.10±0.13	5.17±0.13	499.30±1.12	31.51±0.37
木屑（SD）	11.73±0.21	88.27±0.21	95.82±0.22	6.42±0.06	462.80±1.53	2.31±0.03

[1]	戴征舒, 左元浩, 陈孝罗, 张犁, 赵根, 张学军, 张华. 机器学习在喷射器研究中的应用进展[J]. 化工进展, 2024, 43(S1): 1-12.
[2]	毛宁轩, 万小维, 鞠杰, 胡彦杰, 江浩. 工业气固流化床内流场的CFD-PBM数值模拟和结构优化[J]. 化工进展, 2024, 43(S1): 13-20.
[3]	杨俊辉, 袁君, 张继达, 王金海, 乔红斌, 蔡振义, 马中成. 新型蓄热体结构设计及性能分析[J]. 化工进展, 2024, 43(S1): 282-294.
[4]	周渝, 夏太阳, 韦奇, 唐甜, 田磊. 微通道耦合反渗透膜串联处理甲醇制烯烃废水工艺优化[J]. 化工进展, 2024, 43(S1): 43-51.
[5]	陈王觅, 席北斗, 李鸣晓, 叶美瀛, 侯佳奇, 于承泽, 魏域芳, 孟繁华. 热解系统碳排放削减技术研究进展[J]. 化工进展, 2024, 43(S1): 479-503.
[6]	柳宏伟, 董国亮, 温彦博, 王强华, 徐庆, 王兴盛, 李旭升, 巩洁平, 赵斌, 刘梦瑶. 华亭挤压造粒机组工艺优化[J]. 化工进展, 2024, 43(S1): 52-60.
[7]	郑钧译, 李明, 朱倍弘, 苏畅, 郭思含, 于麒麟, 张耀斌. 餐厨垃圾体系下的丁酸发酵强化[J]. 化工进展, 2024, 43(S1): 597-603.
[8]	陶一, 张晨, 胡益炯, 邱彤. 基于分子结构分布的减压蜡油分子重构模型[J]. 化工进展, 2024, 43(S1): 71-76.
[9]	王亚男, 刘琳琳, 庄钰, 都健. 基于代理模型的环氧乙烷制乙二醇工艺优化同步热集成[J]. 化工进展, 2024, 43(9): 5234-5241.
[10]	吴宇琦, 李江涛, 丁建智, 宋秀兰, 苏冰琴. 焙烧镁铝水滑石脱除厌氧消化沼气中CO₂的效果及机制[J]. 化工进展, 2024, 43(9): 5250-5261.
[11]	张政, 刘琳, 李子晨, 王梦琦, 黄春燕, 葛圆圆. 载铜地质聚合物微球的制备及其催化降解双酚S的性能[J]. 化工进展, 2024, 43(9): 5290-5301.
[12]	罗世发, 王侃, 张冰剑, 陈清林. 原油常减压蒸馏装置热集成方案分析评价[J]. 化工进展, 2024, 43(9): 4810-4816.
[13]	孙启超, 聂美华, 伍联营, 胡仰栋. 风光储一体化水电联产系统的优化设计及调度[J]. 化工进展, 2024, 43(9): 4882-4891.
[14]	王禹程, 郭雄, 栾昕奇, 周健, 李想, 幸林广, 周雪芸, 刘颖, 汪德勇, 吴雪娟, 潘琦, 刘建新, 赵祯霞, 赵钟兴. 维生素U生产工艺优化及其热分解机理[J]. 化工进展, 2024, 43(9): 5157-5167.
[15]	张茜, 李皓芯, 张天阳, 李子富, 孙文俊, 敖秀玮. 基于紫外线的高级氧化或高级还原技术降解水中全氟或多氟烷基化合物[J]. 化工进展, 2024, 43(8): 4587-4600.