羟基磷灰石负载黄芩苷的不同载药方法比较分析

doi:10.16085/j.issn.1000-6613.2021-2368

摘要/Abstract

摘要：

羟基磷灰石在生物医学领域被广泛作为骨修复材料和药物载体，然而目前尚未见到羟基磷灰石负载生物活性因子黄芩苷（baicalin，BA）的相关报道。基于此，本文提出并比较了三种不同的负载方法。通过化学沉淀法和微波水热法分别合成了纳米短棒状羟基磷灰石（nano-hydroxyapatite，nHA）和微米球状羟基磷灰石（micro-hydroxyapatite，mHA），使用这两种不同形貌的羟基磷灰石通过吸附、微波水热一步法合成负载和喷雾干燥三种途径负载黄芩苷。通过TEM、SEM、FTIR、XRD分析了不同载药方式制得的载药羟基磷灰石的形貌、晶体结构和化学成分，对比了三种载药方法的优缺点。结果表明：喷雾干燥制备羟基磷灰石负载BA的载药方式可以在最大程度上保证药物的稳定性，同时达到较高的药物包封率，且这种方法简单快捷，结果重复性好，易于工业化大规模生产。

关键词: 羟基磷灰石, 黄芩苷, 喷雾干燥, 物理吸附, 微波水热

Abstract:

Hydroxyapatite is widely used as a bone repair material and drug carrier in biomedical engineering. However, there are no reports about the hydroxyapatite loaded with the bioactive factor baicalin (BA). Based on this situation, three different loading methods are proposed and compared. In this article, short-rod nano-hydroxyapatite(nHA) and micro-spherical hydroxyapatite(mHA) were synthesized by chemical precipitation method and microwave hydrothermal method, respectively. These two kinds of hydroxyapatite with different morphology were used to load baicalin by physical adsorption, microwave hydrothermal one-step synthesis, and spray drying. Then the morphology, crystal structure and chemical composition of drug-loaded hydroxyapatite prepared by different drug-loading methods were analyzed by TEM, SEM, FTIR, and XRD. Moreover, the advantages and disadvantages of the three drug-loading methods were analyzed. The results show that the spray-drying preparation method of hydroxyapatite-loaded baicalin can ensure the stability of the drug to the greatest extent while achieving a higher drug encapsulation efficiency. This method is simple and rapid with results being reproducible and has advantages of easy industrialization and mass production.

Key words: hydroxyapatite, baicalin, spray drying, physical absorption, microwave hydrothermal

中图分类号:

刘畅, 李玉宝, 左奕, 李吉东. 羟基磷灰石负载黄芩苷的不同载药方法比较分析[J]. 化工进展, 2022, 41(9): 4995-5002.

LIU Chang, LI Yubao, ZUO Yi, LI Jidong. Compare and analysis on different loading methods of hydroxyapatite loaded baicalin[J]. Chemical Industry and Engineering Progress, 2022, 41(9): 4995-5002.

图/表 6

图1 nHA和mHA的理化性能

图2 nHA和mHA吸附负载BA的载药量和包封率

图3 不同BA浓度溶液中制备的载BA的mHA理化性能

图4 不同pH条件下合成的载药mHA的理化性能

图5 喷雾干燥法制得的羟基磷灰石微球的SEM图像及粒径分布图

图6 喷雾干燥法制得的MHA及负载BA的理化性能

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