甘油预处理蔗渣的木质素分离提取及结构表征

doi:10.16085/j.issn.1000-6613.2020-0125

化工进展 ›› 2020, Vol. 39 ›› Issue (11): 4418-4426.DOI: 10.16085/j.issn.1000-6613.2020-0125

甘油预处理蔗渣的木质素分离提取及结构表征

曾诚¹(), 宋国杰¹, 孙海彦², 郭书贤³, 孟超然¹(), 孙付保¹()

^1.江南大学生物工程学院糖化学与生物技术教育部重点实验室，江苏无锡 214122
^2.中国热带农业科学院热带生物技术研究所，海南海口 571101
^3.南阳理工学院生物与化学工程学院河南省工业微生物资源与发酵技术重点实验室，河南南阳 473004

出版日期:2020-11-05 发布日期:2020-11-06
通讯作者: 孟超然,孙付保
作者简介:曾诚（1995—），男，硕士研究生，研究方向为生物质精炼。E-mail：1758800137@qq.com。
基金资助:
江苏省“六大人才高峰”高层次人才项目(XNY-010);国家自然科学基金面上项目(21776114);博士后创新人才支持计划(BX20190146);中国博士后科学基金第65批面上资助项目(2019M651700);江苏省自然科学基金青年基金(BK20190609);农业农村部农村可再生能源开发利用重点实验室开放课题(1014320001190080)

Isolation and structural characterization of glycerol extracted sugarcane bagasse lignin

Cheng ZENG¹(), Guojie SONG¹, Haiyan SUN², Shuxian GUO³, Chaoran MENG¹(), Fubao SUN¹()

^1.Key Laboratory of Sugar Chemistry and Biotechnology, Ministry of Education, College of Bioengineering, Jiangnan University, Wuxi 214122, Jiangsu, China
^2.Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Science, Haikou 571101, Hainan, China
^3.Henan Key Laboratory of Industrial Microbial Resources and Fermentation Technology, School of Biological and Chemical Engineering, Nanyang Institute of Technology, Nanyang 473004, Henan, China

Online:2020-11-05 Published:2020-11-06
Contact: Chaoran MENG,Fubao SUN

摘要/Abstract

摘要：

以甘油分别对甘蔗渣进行常压甘油自催化（AGO）预处理和常压甘油碱催化（al-AGO）预处理。利用酸沉法分别从预处理液中得到自催化甘油木质素（AGOL）和碱催化甘油木质素（al-AGOL）。利用单因素实验和正交实验得到最佳木质素提取工艺为转速8000r/min、离心时间15min、甘油混合液pH为3、甘油混合液浓度10%，在该条件下木质素AGOL和al-AGOL提取率分别达到72%和76%。采用扫描电镜（SEM）、元素分析、紫外光谱（UV）、凝胶色谱（GPC）、核磁共振¹H谱、热重分析以及抗氧化活性分析等技术手段对提取得到的木质素进行结构表征。结果表明：从甘蔗渣中提取的球磨甘蔗木质素（MBL）、AGOL和al-AGOL主要呈现出球形特征；AGOL和al-AGOL具有相似的活性特点，与MBL相比，AGOL和al-AGOL的分子量更小、分布更窄、均一性更好，热稳定性和抗氧化活性更高，有望成为重要的工业原料。

关键词: 甘油预处理, 木质素, 组分分离, 组成结构分析表征

Abstract:

Sugarcane bagasse was pretreated with atmospheric glycerol organosolv (AGO) and alkaline-catalyzed atmospheric glycerol organosolv (al-AGO) process, respectively. Then, their individual lignin, AGO lignin (AGOL) and al-AGO lignin (al-AGOL), was obtained by using acid precipitation method from the pretreatment liquor. With a single-factor and orthogonal experiment, the lignin extraction condition was optimized as follows: centrifugation speed 8000r/min, centrifugation time 15min, pH=3, glycerol solution concentration 10%. Under optimized condition, the extraction of AGOL and al-AGOL reached 72% and 76%, respectively. Scanning electron microscopy (SEM), elemental analysis, ultraviolet spectroscopy (UV), gel permeation chromatography (GPC), ¹H nuclear magnetic resonance spectroscopy and thermogravimetric analysis were used to characterize the structure of extracted lignin. Additionally, the antioxidant activity of these lignin samples was measured. Results showed that three types of lignin, milled bagasse lignin(MBL), AGOL and al-AGOL extracted from sugarcane bagasse predominantly presented a spherical morphology of particles. Compared with MBL, the AGOL and al-AGOL showed smaller molecular weight, narrower distribution, better uniformity, higher thermal stability and antioxidant activity, which are of good promise for using as important industrial raw materials.

Key words: glycerol pretreatment, lignin, composition separation, structure analyze and characterization

中图分类号:

TQ35

曾诚, 宋国杰, 孙海彦, 郭书贤, 孟超然, 孙付保. 甘油预处理蔗渣的木质素分离提取及结构表征[J]. 化工进展, 2020, 39(11): 4418-4426.

Cheng ZENG, Guojie SONG, Haiyan SUN, Shuxian GUO, Chaoran MENG, Fubao SUN. Isolation and structural characterization of glycerol extracted sugarcane bagasse lignin[J]. Chemical Industry and Engineering Progress, 2020, 39(11): 4418-4426.

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甘油预处理蔗渣的木质素分离提取及结构表征

Isolation and structural characterization of glycerol extracted sugarcane bagasse lignin

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