人工湿地-微生物电解池耦合系统的脱氮特性

doi:10.16085/j.issn.1000-6613.2020-0071

摘要/Abstract

摘要：

实验构建了人工湿地-微生物电解池耦合系统（CW-MEC），并考察了CW-MEC在阴极有无曝气及不同水力停留时间（HRT）的条件下对生活污水的处理效果。实验结果显示，降低HRT会让CW-MEC阴极、阳极的COD去除率由91.11%±7.76%、86.58%±9.54%降低为77.81%±14.84%、81.44%±11.11%，氨氮去除率由58.54%±5.80%、58.22%±5.03%降低为48.04%±12.94%、48.27%±13.40%；阴极增加曝气会让CW-MEC阴极、阳极的COD去除率分别提高到89.51%±3.92%、82.40%±1.63%，阴极氨氮去除率提高到71.51%±16.44%，而阳极氨氮去除率不受影响；增加阴极曝气条件后，系统阴、阳极开始有硝酸盐氮积累，而CW-MEC阴极的硝酸盐氮含量明显低于对照组（CW-MFC）；通过电化学性能分析，相对于CW-MFC系统，CW-MEC具有更低的内阻；通过微生物多样性分析，CW-MEC系统具有更丰富的微生物多样性。

关键词: 人工湿地, 微生物电解池, 曝气, 水力停留时间, 微生物多样性

Abstract:

In this study, an innovative constructed wetland-microbial electrolytic cell coupling system (CW-MEC) was developed, and the performance of CW-MEC on treating domestic sewage under the different conditions of cathode aeration and hydraulic retention time (HRT) were investigated. The results showed that the COD removal rate of CW-MEC cathode and anode decreased from 91.11%±7.76%, 86.58%±9.54% to 77.81%±14.84%, 81.44%±11.11%, and ammonia nitrogen removal rates from 58.54%±5.8%, 58.22%±5.03% to 48.04%±12.94% and 48.27%±13.40%. Using additional aeration in the cathode would increase the COD removal rate of C-CW-MEC and A-CW-MEC and the NH₄⁺-N removal rate of C-CW-MEC to 89.51%±3.92%, 82.40%±1.63% and 71.51%±16.44%, respectively. While NH₄⁺-N removal rate of A-CW-MEC was not affected by this condition. When the cathode aeration conditions were added, nitrate nitrogen accumulation began to occur in the cathode and anode of the system, while the nitrate nitrogen content of C-CW-MEC was significantly lower than that of the control group (C-CW- MFC). Through electrochemical performance analysis of both the systems,CW-MEC has lower internal resistance than CW-MFC. Moreover, the microbial diversity analysis showed that the CW-MEC system has richer microbial diversity than the CW-MFC system.

Key words: constructed wetland, microbial electrolytic cell, aeration, hydraulic retention time, microbial diversity

中图分类号:

X703

夏函青, 伍永钢, 付成林, 胡谦. 人工湿地-微生物电解池耦合系统的脱氮特性[J]. 化工进展, 2020, 39(11): 4677-4684.

Hanqing XIA, Yonggang WU, Chenglin FU, Qian HU. Nitrogen removal characteristics of the coupling system of constructed wetland and microbial electrolysis cell[J]. Chemical Industry and Engineering Progress, 2020, 39(11): 4677-4684.

参考文献

1	LOGAN B E, HAMELERS B, ROZENDAL R, et al. Microbial fuel cells: methodology and technology[J]. Environmental Science & Technology, 2006, 40(17): 5181-5192.
2	? SAZ, TüRE C, TüRKER O C, et al. Effect of vegetation type on treatment performance and bioelectric production of constructed wetland modules combined with microbial fuel cell (CW-MFC) treating synthetic wastewater[J]. Environmental Science and Pollution Research, 2018, 25(9): 8777-8792.
3	LIU Hong, GROT S, LOGAN B E. Electrochemically assisted microbial production of hydrogen from acetate[J]. Environmental Science & Technology, 2005, 39(11): 4317-4320.
4	毛政中, 孙怡, 黄志鹏, 等. 微生物电解池产甲烷技术研究进展[J]. 化工学报, 2019, 70(7): 2411-2425.
4	MAO Zhengzhong, SUN Yi, HUANG Zhipeng, et al. Progress of research on methanogenic microbial electrolysis cell[J]. CIESC Journal, 2019, 70(7): 2411-2425.
5	ZHANG Yifeng, ANGELIDAKI I. Microbial electrolysis cells turning to be versatile technology: recent advances and future challenges[J]. Water Research, 2014, 56: 11-25.
6	夏函青, 伍永钢, 江文亭, 等. 人工湿地-微生物燃料电池系统的发展及展望[J]. 化工进展, 2019, 38(12): 1-11.
6	XIA Hanqing, WU Yonggang, JIANG Wenting, et al. Review on development and prospect of constructed wetland coupled with microbial fuel cell[J]. Chemical Industry and Engineering Progress, 2019, 38(12): 1-11.
7	MCCARTY P L. What is the best biological process for nitrogen removal: when and why?[J]. Environmental Science & Technology, 2018, 52(7): 3835-3841.
8	KURT M, DUNN J, BOURNE J R. Biological denitrification of drinking water using autotrophic organisms with H₂ in a fluidized-bed biofilm reactor[J]. Biotechnology and Bioengineering, 1987, 29(4): 493-501.
9	PARK H I, KIM D K, CHOI Y, et al. Nitrate reduction using an electrode as direct electron donor in a biofilm-electrode reactor[J]. Process Biochemistry, 2005, 40(10): 3383-3388.
10	WANG J, SONG X, WANG Y, et al. Microbial community structure of different electrode materials in constructed wetland incorporating microbial fuel cell[J]. Bioresource Technology, 2016, 221: 697-702.
11	DOHERTY L, ZHAO Yaqian, ZHAO Xiaohong, et al. Nutrient and organics removal from swine slurry with simultaneous electricity generation in an alum sludge-based constructed wetland incorporating microbial fuel cell technology[J]. Chemical Engineering Journal, 2015, 266: 74-81.
12	申瑞霞. 微生物电解池处理水热液化废水同步制氢的研究[D]. 北京: 中国农业大学, 2018.
12	SHEN Ruixia. Microbial electrolysis cell for treatment of post-hydrothermal liquefaction wastewater and hydrogen generation[D]. Beijing: China Agricultural University, 2018.
13	FANG Zhou, SONG Hailiang, CANG Ning, et al. Performance of microbial fuel cell coupled constructed wetland system for decolorization of azo dye and bioelectricity generation[J]. Bioresource Technology, 2013, 144: 165-171.
14	范智仁. 人工湿地-微生物燃料电池去除罗丹明B染料[D]. 西安: 长安大学, 2017.
14	FAN Zhiren. Enhanced removal of Rhodamine B (dye) by constructed wetland incorporating microbial fuel cell[D]. Xi’an: Chang’an University, 2017.
15	VIRDIS B, READ S T, RABAEY K, et al. Biofilm stratification during simultaneous nitrification and denitrification (SND) at a biocathode[J]. Bioresource Technology, 2011, 102(1): 334-341.
16	刘畅, 周小康, 徐霞, 等. 单室无膜微生物电解池对厕所废水的脱氮处理[J]. 环境工程学报, 2020, 14(4): 963-969.
16	LIU Chang, ZHOU Xiaokang, XU Xia, et al. Denitrification treatment of single-chamber membrane-free microbial electrolysis cell for toilet wastewater[J]. Chinese Journal of Environmental Engineering, 2020, 14(4): 963-969.
17	顾霞, 黄珊, 陆圆, 等. 不同运行方式对多阳极型微生物燃料电池反硝化及产电性能的影响[J]. 化工进展, 2018, 37(10): 3818-3825.
17	GU Xia, HUANG Shan, LU Yuan, et al. Influence of different operating modes on denitrification and electricity generation in a multi-anode microbial fuel cell[J]. Chemical Industry and Engineering Progress, 2018, 37(10): 3818-3825.
18	赵慧敏, 赵剑强. 不同运行方式对微生物燃料电池处理氨氮废水的影响[J]. 化工进展, 2016, 35(5): 1549-1554.
18	ZHAO Huimin, ZHAO Jianqiang. Influence of different operation modes on ammonia nitrogen wastewater treatment of microbial fuel cell[J]. Chemical Industry and Engineering Progress, 2016, 35(5): 1549-1554.
19	SENGUPTA A, DICK W A. Bacterial community diversity in soil under two tillage practices as determined by pyrosequencing[J]. Microbial Ecology, 2015, 70(3): 853-859.
20	EBRAHIMI S, GABUS S, ROHRBACH-BRANDT E, et al. Performance and microbial community composition dynamics of aerobic granular sludge from sequencing batch bubble column reactors operated at 20℃, 30℃, and 35℃[J]. Applied Microbiology and Biotechnology, 2010, 87(4): 1555-1568.
21	LU Lu, XING Defeng, REN Zhiyong. Microbial community structure accompanied with electricity production in a constructed wetland plant microbial fuel cell[J]. Bioresource Technology, 2015, 195: 115-121.
22	PATUREAU D, BERNET N, MOLETTA R. Effect of oxygen on denitrification in continuous chemostat culture with Comamonas sp SGLY2[J]. Journal of Industrial Microbiology, 1996, 16(2): 124-128.
23	孙棋棋. 侵蚀环境中土壤微生物群落变化特征[D]. 杨陵: 中国科学院大学(中国科学院教育部水土保持与生态环境研究中心), 2018.
23	SUN Qiqi. Variations of soil microbial communities under erosion environment[D]. Yangling: University of Chinese Academy of Sciences (Research Center of Soil and Water Conservation and Ecological Environment), 2018.
24	张文文, 刘秉儒, 牛宋芳. 引黄灌区不同种植年限紫花苜蓿土壤养分与细菌群落特征研究[J]. 草业学报, 2019, 28(5): 46-54.
24	ZHANG Wenwen, LIU Bingru, NIU Songfang. Correlation between soil nutrient status and the bacterial community composition in alfalfa stands of different ages in the Yellow River irrigation area[J]. Acta Prataculturae Sinica, 2019, 28(5): 46-54.
25	赵琛, 张列宇, 马涛, 等. UASB反应器对晚期渗滤液的碳氮协同削减效应[J]. 环境科学研究, 2019, 32(11): 1913-1920.
25	ZHAO Chen, ZHANG Lieyu, MA Tao, et al. The synergistic effect for C/N removal by UASB reactor on mature landfill leachate[J]. Research of Environmental Sciences, 2019, 32(11): 1913-1920.

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