温度对MEC阳极膜形成及胞外聚合物的影响

doi:10.16085/j.issn.1000-6613.2018-2358

摘要/Abstract

摘要：

温度是影响微生物活性和优势菌种以及反应器性能的重要参数之一。实验采用单池微生物电解池（MECs），以厌氧活性污泥为接种物，葡萄糖为碳源，在不同温度条件下培养运行微生物电解池阳极生物膜。实验结果表明：在25℃、30℃、35℃和45℃的培养温度下最大电流密度分别达到2.04A/m²、7.75A/m²、12.27A/m²和2.5A/m²。阳极生物膜电化学活性和氢气产率变化趋势与MECs电流密度相类似，均表现出在一定温度范围内升高温度有利于阳极膜电化学活性与阳极膜氢气产率。进一步分析阳极膜生物量与胞外聚合物（EPS）成分结果表明：一定温度范围内升高温度有利于阳极膜生物量的提高，大量的阳极附着细菌可以产生更高的电流密度与EPS含量，阳极膜EPS中蛋白质成分明显高于多糖，并且蛋白质含量随产电密度增高而增大。傅里叶变换红外光谱（FTIR）分析证实了生物膜EPS中存在蛋白质和碳水化合物。

关键词: 微生物电解池, 阳极生物膜, 温度, 制氢, 胞外聚合物

Abstract:

Temperature was one of the important parameters which can significantly effect on the activity,dominant species and reactor performance. Anaerobic activated sludge was used as the inoculant and glucose was used as carbon source. The anode biofilm of the single chamber microbial electrolysis cells (MECs) was operated at different temperature. The experimental results showed that the maximum current densities were 2.04A/m², 7.75A/m², 12.27A/m² and 2.5A/m² respectively at 25℃, 30℃, 35℃ and 45℃. The trend of electrochemical activity and hydrogen yield of anode biofilm is similar to that of MECs, which shows that increasing temperature in a certain temperature range is beneficial to the electrochemical activity of anode biofilm and hydrogen yield of anode biofilm. The results of anode biomass and extracellular polymeric substances (EPS) analysis indicated that the increase of temperature within a certain temperature range was beneficial to the increase of the anode membrane biomass. A large number of bacteria adhering to the anode could produce higher current density and EPS content. The protein composition of EPS was significantly higher than that of polysaccharide, and the protein content increased with the increase of current density. Fourier transform infrared (FTIR) spectrophotometry analysis confirmed the presence of proteins and carbohydrates in the biofilm.

Key words: microbiological electrolytic cell (MEC), anode biofilm, temperature, hydrogen production, extracellular polymeric substances (EPS)

中图分类号:

X701

孙和临,邵琼丽,李建昌,郑金柱,许坤德,龙宪钢. 温度对MEC阳极膜形成及胞外聚合物的影响[J]. 化工进展, 2019, 38(08): 3809-3815.

Helin SUN,Qiongli SHAO,Jianchang LI,Jingzhu ZHENG,Kunde XU,Xiangang LONG. Influence of temperature on MEC anode biofilm formation and extracellular polymers substances[J]. Chemical Industry and Engineering Progress, 2019, 38(08): 3809-3815.

图/表 9

图1 实验反应装置

图2 不同培养温度下电流密度变化

图3 不同培养温度下MEC产气情况

图4 不同培养温度下MEC发酵末端产物及最终pH变化

图5 不同培养温度下阳极膜的CV曲线图

图6 不同培养下形成阳极生物膜的产氢潜力（无接种物）

图7 不同温度下形成阳极生物膜膜生物量与EPS含量及EPS成分

图8 不同培养温度下阳极膜EPS红外光谱图

表1 EPS红外光谱特征波段指定分子结构

特征吸收波数/cm^-1				指定分子结构
25℃	30℃	35℃	40℃	指定分子结构
3445	3440	3440	3435	O—H与—NH₂伸缩振动
2923	2922	2920	2920	C—H伸缩振动
2851	2851	2580	2851	C—H伸缩振动
1640	1633	1640	1642	—CO伸缩振动 (酰胺Ⅰ区)
1542	1542	1543	1542	—NH平面弯曲—CN伸缩(酰胺Ⅱ区)
1383	1384	1384	1384	—CH₃或O—H平面弯曲
—	—	1234	—	PO核酸或磷酸化蛋白的磷酸二酯键伸缩振动
1035	1040	1044	1040	C—O—P伸缩振动

参考文献 29

1	王博, 高冠道, 李凤祥, 等 . 微生物电解池应用研究进展[J]. 化工进展, 2017, 36(3): 1084-1092.
	WANG Bo , GAO Guandao , LI Fengxiang , et al . Advance in application of microbial electrolysis cells[J]. Chemical Industry and Engineering Progress, 2017, 36(3): 1084-1092.
2	YANG N , HAFEZ H , NAKHLA G . Impact of volatile fatty acids on microbial electrolysis cell performance[J]. Bioresource Technology, 2015, 193(6): 449-455.
3	LIU H , RAMNARAYANAN R , LOGAN B E . Production of electricity during wastewater treatment using a single chamber microbial fuel cell[J]. Environmental Science & Technology, 2006, 38(7): 2281-2285.
4	YOU S , ZHAO Q , ZHANG J , et al . A graphite-granule membrane-less tubular air-cathode microbial fuel cell for power generation under continuously operational conditions[J]. Power Sources, 2007, 173(1): 172-177.
5	JADHAV G S , GHANGREKAR M M . Performance of microbial fuel cell subjected to variation in pH, temperature, external load and substrate concentration[J]. Bioresource Technology, 2009, 100(2): 717-723.
6	CATAL T , KAVANAGH P , O’FLAHERTY V , et al . Generation of electricity in microbial fuel cells at sub-ambient temperatures[J]. Journal of Power Sources, 2011, 196(5): 2676-2681.
7	WAGNER R C , CALL D F , LOGAN B E . Optimal set anode potentials vary in bioelectrochemical systems[J]. Environmental Science & Technology, 2010, 44(16): 6036-6041.
8	FINKELSTEIN D A , TENDER L M , ZEIKUS J G . Effect of electrode potential on electrode-reducing microbiota[J]. Environmental Science & Technology, 2006, 40(22): 6990-6995.
9	KYAZZE G , POPOV A , DINSDALE R , et al . Influence of catholyte pH and temperature on hydrogen production from acetate using a two chamber concentric tubular microbial electrolysis cell[J]. International Journal of Hydrogen Energy, 2010, 35(15): 7716-7722.
10	KIM J R , MIN B , LOGAN B E . Evaluation of procedures to acclimate a microbial fuel cell for electricity production[J]. Applied Microbiology and Biotechnology, 2005, 68(1): 23-30.
11	RITTMANN B E , MCCARTY P L . Environmental biotechnology: principles and applications[M]. Boston: McGraw-Hill, 2001.
12	闫立龙, 刘玉, 任源 . 胞外聚合物对好氧颗粒污泥影响的研究进展[J]. 化工进展, 2013, 32(11): 2744-2748.
	YAN Lilong , LIU Yu , REN Yuan . A review on the effects of extracellular polymeric substance to aerobic granular sludge[J]. Chemical Industry and Engineering Progress, 2013, 32(11): 2744-2748.
13	NAM J Y, KIM H W , LIM K H . Electricity generation from MFCs using differently grown anode-attached bacteria[J]. Environmental Engineering Research, 2010, 15(2): 71-78.
14	VEREA L , SAVADOGO O , VERDE A , et al . Performance of a microbial electrolysis cell (MEC) for hydrogen production with a new process for the biofilm formation[J]. International Journal of Hydrogen Energy, 2014, 39(17): 8938-8946.
15	陆佳, 刘永军, 刘喆, 等 . 有机负荷对污泥胞外聚合物分泌特性及颗粒形成的影响[J]. 化工进展, 2018, 37(4): 1616-1622.
	LU Jia , LIU Yongjun , LIU Zhe , et al . Effects of organic loading on the secretory characteristics of EPS and particle formation[J]. Chemical Industry and Engineering Progress, 2018, 37(4): 1616-1622.
16	张兰河, 王佳平, 陈子成, 等 . Ca²⁺对序批式生物反应器活性污泥性能的影响[J]. 化工进展, 2018, 37(9): 3675-3681.
	ZHANG Lanhe , WANG Jiaping , CHEN Zicheng , et al . Effect of Ca²⁺ on the properties of activated sludge in the SBR[J]. Chemical Industry and Engineering Progress, 2018, 37(9): 3675-3681.
17	王亚利, 刘永军, 刘喆, 等 . 聚合氯化铝投加时间对好氧颗粒污泥的形成和胞外聚合物的影响[J]. 化工进展, 2015, 34(1): 278-284.
	WANG Yali , LIU Yongjun , LIU Zhe , et al . Influence of poly aluminium chloride dosing time on formation and EPS of aerobic granular sludge[J]. Chemical Industry and Engineering Progress, 2015, 34(1): 278-284.
18	KATO S , HASHIMOTO K , WATANABE K . Methanogenesis facilitated by electric syntrophy via (semi) conductive iron-oxide minerals[J]. Environmental Microbiology, 2012, 14(7): 1646-1654.
19	赵煜, 薄晓, 马彦, 等 . 不同温度下微生物燃料电池的运行特性[J]. 化工进展, 2014, 33(3): 629-650.
	ZHAO Yu , BO Xiao , Yan MA , et al . Research on producing electricity characteristics of microbial fuel cell at different temperatures[J]. Chemical Industry and Engineering Progress, 2014, 33(3): 629-650.
20	詹亚力, 王琴, 张佩佩, 等 . 微生物燃料电池影响因素及作用机理探讨[J]. 高等学校化学学报, 2008, 29(1): 144-148.
	ZHAN Yali , WANG Qin , ZHANG Peipei , et al . Investigation on influence factors and mechanism of microbial fuel cell[J]. Chemical Journal of Chinese Universities, 2008, 29(1): 144-148.
21	MARSILI E , ROLLEFSON J B , BARON D B , et al . Microbial biofilm voltammetry: direct electrochemical characterization of catalytic electrode-attached biofilms[J]. Applied & Environmental Microbiology, 2008, 74(23): 7329-7337.
22	VU B, CHEN M , CRAWFORD R J , et al . Bacterial extracellular polysaccharides involved in biofilm formation[J]. Molecules, 2009, 14(7): 2535-2554.
23	ZHANG L , ZHU X , LI J , et al . Biofilm formation and electricity generation of a microbial fuel cell started up under different external resistances[J]. Journal of Power Sources, 2011, 196(15): 6029-6035.
24	REGUERA G , MCCARTHY K D , MEHTA T , et al . Extracellular electron transfer via microbial nanowires[J]. Nature, 2005, 435(7045): 1098-1101.
25	SUN X F , WANG S G , ZHANG X M , et al . Spectroscopic study of Zn²⁺ and Co²⁺ binding to extracellular polymeric substances (EPS) from aerobic granules[J]. Journal of Colloid and Interface Science, 2009, 335(1): 11-17.
26	XU C , ZHANG S , CHUANG C Y , et al . Chemical composition and relative hydrophobicity of microbial exopolymeric substances (EPS) isolated by anion exchange chromatography and their actinide-binding affinities[J]. Marine Chemistry, 2011, 126(1): 27-36.
27	MARUYAMA T , KATOH S , NAKAJIMA M , et al . FT-IR analysis of BSA fouled on ultrafiltration and microfiltration membranes[J]. Journal of Membrane Science, 2001, 192(1): 201-207.
28	OMOIKE A , CHOROVER J . Spectroscopic study of extracellular polymeric substances from bacillus subtilis: aqueous chemistry and adsorption effects[J]. Biomacromolecules, 2004, 5(4): 1219-1230.
29	COMTE S , GUIBAUD G , BAUDU M . Relations between extraction protocols for activated sludge extracellular polymeric substances (EPS) and EPS complexation properties. PartⅠ. Comparison of the efficiency of eight EPS extraction methods[J]. Enzyme and Microbial Technology, 2006, 38(1): 246-252.

[1]	谢璐垚, 陈崧哲, 王来军, 张平. 用于SO₂去极化电解制氢的铂基催化剂[J]. 化工进展, 2023, 42(S1): 299-309.
[2]	徐茂淯, 陶帅, 齐聪, 梁林. 圆盘式环路热管的启动特性及温度波动[J]. 化工进展, 2023, 42(9): 4531-4537.
[3]	时雨, 赵运超, 樊智轩, 蒋达华. 夏热冬冷地区相变屋面最佳相变温度的实验研究[J]. 化工进展, 2023, 42(9): 4828-4836.
[4]	陈翔宇, 卞春林, 肖本益. 温度分级厌氧消化工艺的研究进展[J]. 化工进展, 2023, 42(9): 4872-4881.
[5]	张亚娟, 徐惠, 胡贝, 史星伟. 化学镀法制备NiCoP/rGO/NF高效电解水析氢催化剂[J]. 化工进展, 2023, 42(8): 4275-4282.
[6]	赵健, 卓泽文, 董航, 高文健. 含蜡原油及其乳状液体系微观结构观测的新方法[J]. 化工进展, 2023, 42(8): 4372-4384.
[7]	张雪伟, 黄亚继, 许月阳, 程好强, 朱志成, 李金壘, 丁雪宇, 王圣, 张荣初. 碱性吸附剂对燃煤烟气中SO₃的吸附特性[J]. 化工进展, 2023, 42(7): 3855-3864.
[8]	王蕴青, 杨国锐, 延卫. 过渡金属磷化物的改性方法及其在电化学析氢中的应用[J]. 化工进展, 2023, 42(7): 3532-3549.
[9]	张芳, 郭鹍鹏, 梁春平, 尤雪瑞, 张志超. 一种具有聚集诱导发光效应有机荧光小分子的设计合成及功能应用[J]. 化工进展, 2023, 42(6): 3097-3104.
[10]	符淑瑢, 王丽娜, 王东伟, 刘蕊, 张晓慧, 马占伟. 析氧助催化剂增强光阳极光电催化分解水性能研究进展[J]. 化工进展, 2023, 42(5): 2353-2370.
[11]	戴行, 高瑞雪, 李奕国, 朱锦, 王静刚. 高玻璃化转变温度抗冲击透明聚酯的研究进展[J]. 化工进展, 2023, 42(5): 2555-2565.
[12]	尚小标, 李广超, 肖利平, 白永珍, 肖人友, 李佳剑, 张志浩. 大温度梯度下含锆型硅酸铝纤维板的透波性能[J]. 化工进展, 2023, 42(3): 1551-1561.
[13]	胡璇, 陈滢. 聚酯纤维微塑料胁迫下活性污泥系统性能及微生物群落的变化情况[J]. 化工进展, 2023, 42(2): 1051-1060.
[14]	肖周荣, 李国柱, 王涖, 张香文, 谷建民, 王德松. 液体碳氢燃料蒸汽重整制氢催化剂研究进展[J]. 化工进展, 2022, 41(S1): 97-107.
[15]	胡兵, 徐立军, 何山, 苏昕, 汪继伟. 碳达峰与碳中和目标下PEM电解水制氢研究进展[J]. 化工进展, 2022, 41(9): 4595-4604.