化工进展 ›› 2019, Vol. 38 ›› Issue (08): 3816-3822.DOI: 10.16085/j.issn.1000-6613.2019-0188

• 生物与医药化工 • 上一篇    下一篇

pH对高温厌氧耗氢产甲烷及微生物群落的影响

陈露蕊(),杜诗云,谢丽()   

  1. 同济大学环境科学与工程学院,长江水环境教育部重点实验室,上海 200092
  • 收稿日期:2019-01-29 出版日期:2019-08-05 发布日期:2019-08-05
  • 通讯作者: 谢丽
  • 作者简介:陈露蕊(1996—),女,硕士研究生,研究方向为厌氧生物处理技术。E-mail:1732795@tongji.edu.cn
  • 基金资助:
    国家自然科学基金(51678424)

Effect of pH on hydrogenotrophic methanogenesis and microbial community under thermophilic condition

Lurui CHEN(),Shiyun DU,Li XIE()   

  1. The Yangtze River Water Environment Key Laboratory of the Ministry of Education, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China
  • Received:2019-01-29 Online:2019-08-05 Published:2019-08-05
  • Contact: Li XIE

摘要:

随着化石燃料的枯竭,以厌氧耗氢产甲烷菌为功能优势菌群的异位(或离位)加氢沼气生物提纯工艺备受关注。本文考察了高温条件下不同初始pH对厌氧耗氢产甲烷过程及微生物群落的影响。研究结果表明,半连续流反应器中不同初始pH变化对产甲烷量影响不大,在以H2/CO2为基质的厌氧体系中,厌氧耗氢产甲烷过程是甲烷产生的主要途径。比产甲烷活性研究结果进一步表明碱性条件(初始pH=8.5~9.0)耗氢产甲烷污泥对氢气消耗率提高,产甲烷迟滞期缩短至6.9h,且甲烷产率高达19.8mL CH4 /(gVS?h)。通过高通量测序技术对不同初始pH条件下的产甲烷古菌群落进行分析,3个样本均以厌氧耗氢产甲烷菌为主导,酸性和中性条件古菌群落属水平上相似,以Methanothermobacter为主,其相对丰度分别为90.6%、91.6%;而碱性条件下以Methanobacterium为主,其相对丰度可达83.6%,还发现了Methanomassiliicoccus,其相对丰度可达7.7%碱性条件下Methanobacterium相对丰度的提高和Methanomassiliicoccus的富集,可能是碱性条件下比产甲烷活性提高的主要原因。

关键词: 耗氢产甲烷, 高温, pH, 比产甲烷活性, 微生物群落

Abstract:

Ex-situ biogas upgrading based on hydrogenotrophic methanogenic process has attracted much attention with the depletion of fossil fuels. This study investigated effects of initial pH on anaerobic hydrogen-consuming methanogenesis and microbial community at 55oC. Experimental results showed that variations of initial pH did not influence methane production under semi-continuous condition. Hydrogenotrophic methanogenesis was found to be the main pathway of methanogenesis under the tested conditions at the anaerobic system with H2/CO2 as the matrix. Alkaline condition (pH 8.5―9.0) improved the hydrogen consumption, and decreased lag phase of methane production to 6.9h, and methane yield was as high as 19.8mL CH4 /(gVS?h). High throughput sequencing results further demonstrated that hydrogenotrophic methanogens was the main species. Methanothermobacter, with relative abundance of 90.6%―91.6% was found to dominate under acidic and neutral conditions. While Methanobacterium with relative abundance of 83% was the main species under alkaline condition. Methanomassiliicoccus was also detected, with relative abundance of 7.7%. The relative abundance of Methanobacterium and the enrichment of Methanomassicicoccus might be contributable to the high specific methane activity under alkaline condition.

Key words: hydrogenotrophic methanogenesis, thermophilic, pH, specific methanogenic activity, microbial community

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