固定化细胞技术应用于酒精发酵中的研究进展

doi:10.16085/j.issn.1000-6613.2017.04.033

摘要/Abstract

摘要： 固定化细胞技术出现在20世纪70年代后期，是在固定化酶基础上发展起来的。近年来，固定化细胞技术被广泛应用于燃料酒精的研究与生产中。因其可以反复使用、连续发酵以及提高酒精得率等优势显示了巨大的发展潜力。研究固定化细胞酒精发酵具有十分重要的现实意义。本文综述了固定化细胞技术在酒精发酵领域的研究进展，介绍了酒精生产中常用的细胞固定化方法、特性及优势、固定化细胞技术在酒精发酵中应用以及在酒精发酵中存在的问题及解决办法等。指出开展细胞与细胞、细胞与酶的共固定化技术，无载体自絮凝细胞固定化技术以及开发新型酒精专用固定化载体材料是未来固定化细胞技术在酒精工业规模化应用的关键。

关键词: 生物工程, 固定化, 酒精, 发酵

Abstract: Cell immobilization technology was developed in 1970's based on the enzyme immobilization. Recently,cell immobilization is widely used in research and production of fuel ethanol. It demonstrated great potential with advantages of repeated applications,continuous operations,and high ethanol yield. So researches on cell immobilization in ethanol production are very important for practical applications. This article reviewed research progresses on cell immobilization in ethanol production. The article discussed the common methods,characteristics and advantages and applications of cell immobilization,problems,and solutions. The keys for future ethanol productions are developing co-immobilization of cell-cell,cell-enzyme and self-immobilization cell technologies, and developing new immobilization support materials for ethanol production.

Key words: biological engineering, immobilization, ethanol, fermentation

中图分类号:

张强, 嵇冶. 固定化细胞技术应用于酒精发酵中的研究进展[J]. 化工进展, 2017, 36(04): 1404-1409.

ZHANG Qiang, JI Ye. Research progress on cell immobilization technology in ethanol production[J]. Chemical Industry and Engineering Progress, 2017, 36(04): 1404-1409.

参考文献

[1] 李振宇,李顶杰,黄格省,等. 燃料酒精发展现状及思考[J]. 化工进展,2013,32(7):1457-1467. LI Z Y,LI D J,HUANG G S,et al. Insights on current development of fuel ethanol[J]. Chemical Industry and Engineering Progress,2013,32(7):1457-1467.
[2] 毕生雷,张成明.固定化技术在生物能源及环境工程领域的研究和应用进展[J]. 酿酒科技,2015(1):102-105. BI S l,ZHANG C M. Research and application progress of immobilization technology in bio-energy and environmental engineering[J]. Liquor-making Science &Technology,2015(1):102-105.
[3] MA M G. Mechanisms of ethanol tolerance in Saccharomyces cerevisiae[J]. Applied Microbiology and Biotechnology,2010,87(3):829-845.
[4] 高培培,胡纯铿,洪美,等. 酵母固定化技术在燃料乙醇生产中的应用[J]. 食品与发酵工业,2010,36(3):125-128. GAO P P,HU C K,HONG M,et al. Yeast immobilization technologies applied to fuel ethanol production[J]. Food and Fermentation Industries,2010,36(3):125-128.
[5] 李丹,刘冬梅,于淑娟.泡沫多孔陶瓷固定酵母细胞条件研究[J].现代食品科技,2008,25(12):1400-1403. LI D,LIU D M,YU S J. Immobilization of yeast on porous foamed ceramics[J]. Modern Food Science & Technology,2008,25(12):1400-1403.
[6] 程江峰,苏忠亮,梁成伟.泡沫陶瓷固定化啤酒酵母工艺[J]. 中国酿造,2009,20(3):109-111. CHENG J F,SU Z L,LIANG C W. Immobilization technology of Saccharomyces cerevisiae in foam ceramics[J]. China Brewing,2009,28(3):109-111.
[7] 尤业兵,缪冶炼,陈介余.海藻酸钙凝胶颗粒固定化抗冻酵母AFY-1的机制分析及条件优化[J].生物加工过程,2006(4):88-93. YOU Y B,MIAO Y L,CHEN J Y. Mechanism analysis and condition optimization for the immobilization of freeze-tolerant yeast AFY-1 within Ca-alginate gel particles[J].Bioprocessing Engineering,2006(4):88-93.
[8] STRATFORD M. Evidence for two mechanisms of flocculation in Saccharomyces cerevisiae[J].Yeast,1989(5):441-445.
[9] VERSTREPEN K J,KLIS F M. Flocculation,adhesion and biofilm formation in yeasts[J].Mol. Microbiol.,2006(6):5-15.
[10] 白凤武.无载体固定化细胞的研究进展[J].生物工程进展,2000,20(2):431-435. BAI F W. Application of self-immobilization cell technology for biochemical engineering[J].Progress in Biotechnology,2000,20(2):431-435.
[11] 张长利,王景晶,杨宏. 细胞固定化技术研究进展及其在水处理领域的应用[J].水处理技术,2013,39(6):1-3. ZHANG C L,WANG J J,YANG H. Review on immobilized cell technology and its application in water treatment[J].Technology of Water Treatment,2013,39(6):1-3.
[12] 秦晴. 自絮凝酵母超高浓度乙醇发酵及其生理生化研究[D].厦门:华侨大学,2008. QIN Q. A study on very-high-gravity ethanol fermentation and its related physiological and biochemical aspects of self-flocculating yeast[D]. Xiamen:Huaqiao University,2008.
[13] 伍彦华,覃红梅.固定化酵母的选育及在木薯浓醪发酵生产燃料乙醇中的性能对比[J]. 酿酒科技,2010(7):31-33. WU Y H,QIN H M. Breeding of immobilized yeasts & comparison of their performance in high-gravity fermentation of cassava to produce fuel ethanol[J]. Liquor-making Science&Technology,2010(7):31-33.
[14] 刘芳,于建生. 细胞固定化及其在酿酒工业中的应用研究进展[J].酿酒科技,2013(8):85-87. LIU F,YU J S. Research progress in cell immobilization and its application in liquor-making[J]. Liquor-making Science & Technology,2013(8):85-87.
[15] 樊婧,刘慧霞.不同温度对甘蔗糖料固定化酒精发酵的影响[J]. 中国酿造,2010,215():114-116. FAN J,LIU H X. Effects of temperatures on alcohol production with immobilized fermentation of sugarcane feedstocks[J]. China Brewing,2010,29(2):114-115.
[16] MARTINI E,ANDRIANI D,GOBIKRISHNAN S. Immobilization of Saccharomyces cerevisiae in rice hulls for ethanol production[J]. Makara Seri Teknologi,2012,14(2):230-236.
[17] 叶世超,薛婷.固定化酵母R36酒精发酵工艺研究[J].中国酿造,2013,25(8):65-68. YE S C,XUE T. Technology of immobilized yeast R36 alcoholic fermentation[J]. China Brewing,2013,25(8):65-68.
[18] 张强.餐厨垃圾同步糖化发酵生产燃料酒精的工艺[J].化工进展,2015,34(1):91-94. ZHANG Q. Fuel ethanol production from kitchen garbage by simultaneous Saccharification and fermentation[J]. Chemical Industry and Engineering Progress,2015,34(1):91-94,126.
[19] 熊亚. 固定化酵母菌与游离酵母菌产酒精作用的比较研究[J].西昌学院学报,2008,22(2):45-48. XIONG Y. Study on comparison of alcohol fermentation by immobilized yeast and free yeast[J].Journal of Xichang College,2008,22(2):45-48.
[20] 陈明,王周芳,夏黎明.固定化基因重组酵母发酵木糖产乙醇[J].浙江大学学报,2008,42(2):290-293. CHEN M,WANG Z F,XIA L M. Ethanol fermentation on xylose by immobilized recombinant yeast[J].Journal of Zhejiang University,2008,42(2):290-293.
[21] 汪东武,王治业.固定化酵母在甘蔗糖蜜酒精发酵中的问题探讨[J].甘肃科学学报,2006,18(1):66-69. WANG D W,WANG Z Y. Discussion on problems of immobilized yeast in alcoholic fermentation from sugar molasses[J].Journal of Gansu Sciences,2006,18(1):66-69.
[22] 李文.固定化酵母发酵性能调控及在制备燃料乙醇中的应用[D]. 西安:长安大学,2014. LI W. Production of bioethanol onto enhanced fermentation performance of immobilized yeast[D]. Xi'an:Changan University,2014.
[23] 王克明. 共固定化多菌种酒精发酵条件的研究[J].中国调味品,2000,(12):10-12. WANG K M. Study on condition of alcoholic fermentation with co-immobilized multi-microoganisms[J]. China Condiment,2000(12):10-12.
[24] LEE S E,KIM Y O. Two-step process using immobilized Pichia stipitis for ethanol production from Ulva kjellman hydrolysate[J]. Journal of Microbiology and Biotechnology,2013,23(10):1434-1444.
[25] LEBEAU T,JOUENNE T,JUNTER G A. Continuous alcoholic fermentation of glucose/xylose mixtures by co-immobilized Saccharomyces cerevisiae and Candida shehatae[J]. Appl. Microbiol. Biotechnol.,1998(50):309-313.
[26] 李魁.酵母菌与糖化酶共固定化木薯酒精连续发酵工艺研究[J].中国粮油学报,2005,20(1):36-40. LI K. Alcoholic continuous fermentation by co-immobilization of saccharifying enzymes and yeasts[J].Journal of Chinese Cereals and Oils Association,2005,20(1):36-40.
[27] BANDARU V V R,SOMALANKA S R,MENDU D R,et al. Optimization of fermentation conditions for the production of ethanol from sago starch by co-immobilized amyloglucosidase and cells of Zymomonas mobilis using response surface methodology[J]. Enzyme and Microbial Technology,2006,38(1/2):209-214.
[28] EVRIM GÜNE? A. Ethanol production from starch by co-immobilized amyloglucosidase——Zymomonas Mobilis cells in a continuously-stirred bioreactor[J].Biotechnology & Biotechnological Equipment,2013,27(1):3506-3512.
[29] 侯红萍,闫跃文.吸附-包埋结合法共固定化糖化酶和酵母菌的研究[J]. 食品科学,2009,30(5):201-204. HOU H P,YAN Y W. Co-immobilization of glucoamylase and yeast by adsorption-embedment method[J]. Food Science,2009,30(5):201-204.
[30] 薄涛,侯红萍.共固定化技术研究进展[J]. 酿酒科技,2010(7):77-79. BO T,HOU H P. Research progress in co-immobilization technology[J]. Liquor-making Science&Technology,2010(7):77-79
[31] 李超敏,韩梅,张良,等.细胞固定化技术——海藻酸钠包埋法的研究进展[J]. 安徽农业科学,2006,34(7):1281-1284. LI C M,HAN M,ZHANG L,et al. Development of sodium aliginate immobilized cell technology[J]. Journal of Anhui Agricultural Science,2006,34(7):1281-1284.
[32] 张秀霞,秦丽姣.微生物固定化载体的选择及其性能[J].化工进展,2011,30(12):2781-2784. ZHANG X X,QIN L J. Study on the selection of immobilized carrier and its performance[J]. Chemical Industry and Engineering Progress,2011,30(12):2781-2784.