棉布支架固定化米根霉联产果胶酶及用于处理烟梗

doi:10.16085/j.issn.1000-6613.2016.05.34

化工进展 ›› 2016, Vol. 35 ›› Issue (05): 1494-1501.DOI: 10.16085/j.issn.1000-6613.2016.05.34

棉布支架固定化米根霉联产果胶酶及用于处理烟梗

何源, 郑羽西, 潘君, 王远亮

生物流变科学与技术教育部重点实验室生物材料与仿生工程中心重庆大学生物工程学院, 重庆 400044

收稿日期:2015-11-10 修回日期:2015-12-24 出版日期:2016-05-05 发布日期:2016-05-05
通讯作者: 王远亮,教授,博士生导师,从事组织工程与生物材料。E-mail wyl@cqu.edu.cn。
作者简介:何源(1990-),男,硕士研究生
基金资助:
国家自然科学基金(30870609)及重庆市自然科学基金重点项目(CSTC2012JJB90009)。

Pectinase production with Rhizopus oryzae mycelium immobilized on cotton matrix and treatment of tobacco stem

HE Yuan, ZHENG Yuxi, PAN Jun, WANG Yuanliang

Key Laboratory of Biorheological Science and Technology, Ministry of Education Center of Bioinspired Material Science and Engineering; Bioengineering College, Chongqing University, Chongqing 400044, China

Received:2015-11-10 Revised:2015-12-24 Online:2016-05-05 Published:2016-05-05

摘要/Abstract

摘要： 利用果胶酶处理烟梗纤维生产乳酸等对环境友好,而应用新的发酵组合方式和优化发酵条件是提高产酶量,降低成本的有效途径。本文采用实验室研发的棉布支架固定化米根霉细胞的技术,优化了底物为果胶或烟梗的发酵产果胶酶的培养条件,还优化了处理烟梗果胶的条件。底物为果胶的最佳产酶条件为:转速190r/min,装液量50mL/250mL,发酵温度30℃,初始pH值5,初始孢子浓度0.75×10⁶个/mL。底物为烟梗的最佳产酶条件为:初始pH值4.6、初始孢子浓度0.5×10⁶个/mL等。在优化处理烟梗果胶的条件下,固定化米根霉产果胶酶连续法比游离的果胶酶法降解烟梗果胶的效果好,果胶降解率提高18.5%,达到74.1%。棉布支架固定化米根霉利用果胶发酵产果胶酶量较高,利用烟梗发酵脱胶效果较好。

关键词: 米根霉, 果胶酶, 固定化, 生物脱胶, 烟梗

Abstract: The use of pectinases causes less environmental pollution in tobacco stem fiber processing for the production of lactic acid, etc. The effective way to improve pectinase production and reduce cost is to use a new combined fermentation mode and optimize its conditions. Mycelia of Rhizopus oryzaewere immobilized on the cotton matrix by immobilized cells technology developed in our laboratory. Conditions of pectinase production were optimized in culture medium of pectin or tobacco stem and the treatments of tobacco stem pectin were also optimized. The optimal fermentation conditions in pectin medium were rotary speed of 190r/min, liquid volume of 50mL/250mL, temperature of 30℃, pH value of 5, and spore concentration of 0.75×10⁶/mL. The optimal fermentation conditions in tobacco stem medium were, pH value of 4.6, spore concentration of 0.5×10⁶/mL, etc. Under the optimal treatment conditions of tobacco stem pectin, immobilized Rhizopus oryzaecontinuous processing method showed a better pectin degradation performance than the free pectinases processing method with the pectin degradation rate increased by 18.5%, up to 74.1%. Pectinase production with Rhizopus oryzae mycelium immobilized on the cotton matrix showed a higher pectinase production in pectin medium and better tobacco stem fiber degumming in tobacco stem fermentation.

Key words: Rhizopus oryzae, pectinase, immobilization, bio-degumming, tobacco stem

中图分类号:

TQ920.6

何源, 郑羽西, 潘君, 王远亮. 棉布支架固定化米根霉联产果胶酶及用于处理烟梗[J]. 化工进展, 2016, 35(05): 1494-1501.

HE Yuan, ZHENG Yuxi, PAN Jun, WANG Yuanliang . Pectinase production with Rhizopus oryzae mycelium immobilized on cotton matrix and treatment of tobacco stem[J]. Chemical Industry and Engineering Progree, 2016, 35(05): 1494-1501.

参考文献

[1] 任民, 王志德, 牟建民, 等. 我国烟草种质资源的种类与分布概况[J]. 中国烟草科学, 2009, 30(s1): 8-14.
[2] 董占能, 白聚川, 张皓东. 烟草废弃物资源化[J]. 中国烟草科学, 2008, 29(1): 39-42.
[3] 鲁蕾, 付敏, 郭宝星. 烟梗成分提取及其应用研究[J]. 四川化工, 2004, 7(1): 9-11.
[4] MOSIER N, WYMAN C, DALE B, et al. Features of promising technologies for pretreatment of lignocellulosic biomass[J]. Bioresource Technology, 2005, 96(6): 673-686.
[5] CAFFALL K H, MOHNEN D. The structure, function, and biosynthesis of plant cell wall pectic polysaccharides[J]. Carbohydrate Research, 2009, 344(14): 1879-1900.
[6] 陈洪章. 纤维素生物技术[M]. 北京: 化学工业出版社, 2015.
[7] BERLIN A, MAXIMENKO V, GILKES N, et al. Optimization of enzyme complexes for lignocellulose hydrolysis[J]. Biotechnology and Bioengineering, 2007, 97(2): 287-296.
[8] RUDOLF A, ALKASRAWI M, ZACCHI G, et al. A comparison between batch and fed-batch simultaneous saccharification and fermentation of steam pretreated spruce[J]. Enzyme Microb. Technol., 2005, 37(2): 195-204.
[9] 冯洪建, 黄国林. 果胶提取的研究进展[J]. 广东化工, 2013, 40(21): 84-85.
[10] GRAY K A, Zhao L S, EMPTAGE M. Bioethanol[J]. Current Opinion in Chemical Biology, 2006, 10(2): 141-146.
[11] PARIDAH M T, BASHER A B, SAIFULAZRY S, et al. Retting process of some bast plant fibers and its effect on fiber quality: a review[J]. Bioresources, 2011, 6(4): 5260-5281.
[12] ZHANG J, HENRIKSSON H, SZABO I J, et al. The active component in the flax-retting system of the zygomycete Rhizopus oryzae sb is a family 28 polygalacturonase[J]. J. Ind. Microbiol. Biotechnol., 2005, 32(10): 431-438.
[13] 李祖明, 张洪勋, 白志辉, 等. 微生物果胶酶研究进展[J]. 生物技术通报, 2010, 80(3): 42-49.
[14] GHOSH B, RAY R R. Current commercial perspective of Rhizopus oryzae: a review[J]. Journal of Applied Science, 2011, 11(14): 2470-2486.
[15] JAYANI R S, SAXENA S, GUPTA R. Microbial pectinolytic enzymes: a review[J]. Process Biochemistry, 2005, 40(9): 2931-2944.
[16] HART H E, PARISH M E, BUMS J K, et al. Orange finisher pulp as
substrate for polygalacturonase production by Rhizopus oryzae [J]. J. Food Sci., 1991, 56(2): 480-483.
[17] HAMDY H S. Purification and characterization of the pectin lyase secreted within the macerating fluid of Rhizopus oryzae(Went, Prinsen Geerlings)grown on orange peel[J]. Indian J. Biotechnol., 2006, 5(3): 284-291.
[18] KAREEM S O, ADEBOWALE A A. Clarification of orange juice by crud fungal pectinase from citrus peel[J]. Nig. Food J., 2007, 25(1): 130-137.
[19] LAN T Q, WEI D, YANG S T, et al. Enhanced cellulase production by Trichoderma viride in a rotating fibrous bed bioreactor[J]. Bioresource Technology, 2013, 133: 175-182.
[20] IBRAHIM D, WELOOSAMY H, SHEH-HONG L. Potential use of nylon scouring pad cubes attachment method for pectinase production by Aspergillus niger HFD5A-1[J]. Process Biochemistry, 2014, 49(4): 660-667.
[21] 王润光, 王远亮, 王珍, 等. 一种新型支架在乳酸发酵中的应用[J]. 化工进展, 2010, 29(4): 733-738.
[22] MARCIA M C N S, ROBERTO S, Eleni Gomes. Screening of bacterial strains for pectinolytic activity: characterization of the polygalacturonase produced byBacillus sp.[J].Revista de Microbiologia, 1999, 30: 299-303.
[23] TUTTOBELLO R, MILL P J. The pectic enzymes of Aspergillus niger: the production of active mixtures of pectic enzymes[J]. Biochemical Journal, 1961, 79(1): 51-57.
[24] MILLER G L. Use of dinitrosalicylic acid reagent for determination of reducing sugars[J]. Analytical Chemistry, 1959, 31(3): 426-428.
[25] 王小敏, 吴文龙, 闾连飞, 等. 分光光度计法测定果胶酶活力的方法研究[J]. 食品工业科技, 2007, 28(5): 227-229.
[26] 张凤仙, 刘梅芳. 15种植物果胶含量及甲氧基的测定[J]. 植物学通报, 1995(12): 69-70.
[27] 杨胜. 饲料分析及饲料质量检测技术[M]. 北京: 中国农业大学出版社, 1993.
[28] SAITO K, TAKAKUWA N, ODA Y. Purification of the extracellular pectinolytic enzyme from the fungus Rhizopus oryzae NBRC 4707[J]. Microbiological Research, 2004, 159(1): 83-86.
[29] HAMDY H S. Purification and characterization of the pectin lyase produced by Rhizopus oryzaegrown on orange peels[J]. Ann. Microbiol., 2005, 55(3): 205-211.
[30] ZENI J, CENCE K, GRANDO C E, et al. Screening of pectinase-producing microorganisms with polygalacturonase activity[J]. Applied Biochemistry and Biotechnology, 2011, 163(3): 383-392.
[31] CORDEIRO C A M, MARTINS M L L. Produção de poligalacturonase, pelo termofílico Bacillus sp. e algumas de suas propriedades[J]. Ciência e Tecnologia de Alimentos, 2009, 29(1): 135-141.
[32] KAPOOR M, BEG Q K, BHUSHAN B, et al. Production and partial purification and characterization of a thermo-alkalistable polygalacturonase fromBacillus sp. MG-cp-2[J]. Process Biochemistry, 2000, 36(5): 467-473.
[33] 刘耀飞, 常纪恒, 于川. 烟梗中果胶的酶法降解[J]. 烟草科技, 2013(8): 40-44.
[34] 杨慧芳. 降解烟梗果胶质微生物筛选及产果胶酶的研究[D]. 无锡: 江南大学, 2012.
[35] 阮昕, 李彪, 候读成, 等. 超声波耦合酶反应去除烟梗中果胶和纤维素的工艺优化[J]. 贵州农业科学, 2015, 43(2): 145-149.
[36] 王珍. 一种新型棉布载体在固定化米根霉发酵生产L-乳酸的研究[D]. 重庆: 重庆大学, 2010.

棉布支架固定化米根霉联产果胶酶及用于处理烟梗

Pectinase production with Rhizopus oryzae mycelium immobilized on cotton matrix and treatment of tobacco stem

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