Chemical Industry and Engineering Progress ›› 2023, Vol. 42 ›› Issue (6): 3157-3166.DOI: 10.16085/j.issn.1000-6613.2022-1404

• Materials science and technology • Previous Articles     Next Articles

Preparation and application of molecularly imprinted polymers of terbutylazine assisted by computer simulation

YANG Farong1(), GU Lili1(), LIU Yang1, LI Weixue1, CAI Jieyun2, WANG Huiping2   

  1. 1.Faculty of Chemical Engineering, Kunming University of Science and Technology, Kunming 650500, Yunnan, China
    2.Yunnan Tobacco Quality Supervision and Testing Station, Kunming 650106, Yunnan, China
  • Received:2022-07-26 Revised:2022-08-31 Online:2023-06-29 Published:2023-06-25
  • Contact: GU Lili

计算机模拟辅助特丁津分子印迹聚合物的制备及应用

杨发容1(), 顾丽莉1(), 刘洋1, 李伟雪1, 蔡洁云2, 王惠平2   

  1. 1.昆明理工大学化学工程学院,云南 昆明 650500
    2.云南省烟草质量监督检测站,云南 昆明 650106
  • 通讯作者: 顾丽莉
  • 作者简介:杨发容(1996—),女,硕士研究生,研究方向为天然产物的分离与提纯。E-mail:906397946@qq.com
  • 基金资助:
    云南省烟草公司科技计划(2021530000241009)

Abstract:

A method of molecularly imprinted solid phase extraction combined with high performance liquid chromatography (MISPE-HPLC) was developed for the determination of two triazine herbicide residues in tobacco leaves. An optimal functional monomer was firstly selected and then the optimum polymerization ratio of the template molecule (TER) to the functional monomer (AA) was determined by computer simulation technology. Based on the simulation results, the molecularly imprinted polymers of terbutylazine were prepared by precipitation polymerization. Comparing the infrared spectra of the computer-simulated complex with the experimentally prepared TER-MIPs, the analysis showed that the main absorbing groups of them had the same peak positions. The adsorption performances of MIPs to the template molecule and its structural analogs were investigated. It was found that MIPs had good specific adsorption capacities for TER and its structural analogs. TER-MIPs was used as a solid phase extraction filler to prepare a TER-MISPE column for pretreatment of spiked tobacco leaves and the samples were analyzed by HPLC. The results indicated that MISPE had better enrichment effect on terbutylazine and terbumeton. The average recoveries of the two triazine herbicides were between 84.29% and 96.64% and the relative standard deviations (RSD) were 1.19%—1.70% (n=3). The method can meet the requirements of simultaneous determination of terbutylazine and terbumeton triazine pesticide residues in tobacco leaves, providing support for the analysis and detection of triazine herbicides in tobacco leaves and crops with complex substrates.

Key words: terbutylazine, molecular simulation, polymers, adsorption, pesticide residues

摘要:

建立了烟叶中两种三嗪类除草剂残留的分子印迹固相萃取-高效液相色谱(MISPE-HPLC)检测方法。利用计算机模拟技术筛选最佳功能单体,确定模板(TER)与单体(AA)的最优结合比例,以模拟结果为依据,采用沉淀聚合制备了特丁津分子印迹聚合物。对比了计算机模拟复合物与实验制备的TER-MIPs红外光谱图,分析表明,两者的主要吸收基团出峰位置一致。考察了MIPs对模板分子的吸附性能,结果显示,MIPs对TER及其结构类似物具有良好的特异性吸附能力。将TER-MIPs作为固相萃取填料,制备TER-MISPE柱对加标烟叶进行前处理,用高效液相色谱(HPLC)分析前处理液。结果表明,MISPE对特丁津及特丁通的富集效果较好,两种三嗪类除草剂的回收率为84.29%~96.64%,RSD为1.19%~1.70%(n=3),该方法满足烟叶中特丁津及特丁通两种三嗪类农药残留的同时检测,为烟叶及复杂基质农作物中三嗪类除草剂的分析检测提供了支撑。

关键词: 特丁津, 分子模拟, 聚合物, 吸附, 农药残留

CLC Number: 

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