羧甲基纤维素-大豆分离蛋白农药缓释颗粒的制备及性能

doi:10.16085/j.issn.1000-6613.2020-1248

摘要/Abstract

摘要：

为提高农药利用率、精确控制农药释放，设计了一种pH响应型缓释颗粒。以3-氨丙基三乙氧基硅烷（APTES）为桥连接羧甲基纤维素（CMC）与大豆分离蛋白（SP）得到羧甲基纤维素-大豆分离蛋白（CMC-SP），然后利用分子自组装法负载阿维菌素（AVM）形成载药颗粒（CMC-SP@AVM）。采用红外光谱（FTIR）、扫描电镜（SEM）、热重分析（TGA）等手段对改性产物结构和形貌进行表征，并对CMC-SP@AVM的载药性能、缓释性能、抗紫外性能、杀虫活性进行了探究。结果表明，CMC-SP@AVM具有近似椭圆形的结构，CMC-SP@AVM的平均粒径为104nm；对AVM的包封率达41.9%，并赋予AVM优异的抗紫外光分解性能，强紫外光照射120h后，CMC-SP@AVM中AVM的残留率比未包封的AVM高117%，其药物释放具有pH响应特性，pH越大，释放速率越快；药物释放过程符合Elovich模型。在相同AVM浓度下CMC-SP@AVM的杀虫活性与原药无显著差异。

关键词: 载体, 蛋白质, 纳米结构, 阿维菌素, pH响应

Abstract:

In order to improve pesticide utilization and control pesticide release, a pH responsive particle was designed. 3-aminopropyl triethoxysilane (APTES) was used as a bridge to connect carboxymethyl cellulose (CMC) and soybean protein isolate (SP). Avermectin (AVM) was hydrophobically encapsulated as a model drug to obtain CMC-SP@AVM. The chemical interaction between SP and CMC was confirmed by Fourier transform infrared (FTIR), thermogravimetric analysis (TGA), and differential scanning calorimetric (DSC). Their structural characteristics and stability were investigated through scanning electron microscope (SEM) and zeta potential technique . It found that AVM encapsulated in CMC-SP@AVM particles with average size of 104nm. Ultraviolet-visible (UV-vis) spectroscopy was employed to explore the anti-UV property and encapsulation as well as release behavior, which revealed that the presence of CMC-SP@AVM protected AVM from UV photolysis with encapsulation efficiency of 41.9%. The residual rate of AVM in the CMC-SP@AVM was 117% higher than that of unsealed AVM after irradiating with strong ultraviolet light for 120h. The release rate of AVM was pH responsive. The higher the pH, the faster the release rate. It showed that the release of AVM from CMC-SP@AVM fit to the Elovich kinetic model. The insecticidal activity of CMC-SP@AVM is not significantly different from that of the original drug at the same AVM concentration.

Key words: carrier, protein, nanostructures, avermectin, pH response

中图分类号:

TQ450.6

李梓泳, 马憬希, 赵明, 陈龙, 周红军, 周新华. 羧甲基纤维素-大豆分离蛋白农药缓释颗粒的制备及性能[J]. 化工进展, 2021, 40(5): 2739-2746.

LI Ziyong, MA Jingxi, ZHAO Ming, CHEN Long, ZHOU Hongjun, ZHOU Xinhua. Preparation and performance of carboxymethyl cellulose-soybean protein isolate pesticides sustained-release particles[J]. Chemical Industry and Engineering Progress, 2021, 40(5): 2739-2746.

图/表 11

参考文献 33

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m_CMC∶m_SP	电位/mV	平均粒径/nm	包封率/%
2∶1	-15.02±0.93	238.13±25.06	41.9
1∶1	-20.79±0.25	267.48±12.63	39.6
1∶2	-22.91±0.04	361.49±10.29	33.1

m_CMC∶m_SP	电位/mV	平均粒径/nm	包封率/%
2∶1	-15.02±0.93	238.13±25.06	41.9
1∶1	-20.79±0.25	267.48±12.63	39.6
1∶2	-22.91±0.04	361.49±10.29	33.1

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