纳米纤维素晶须-壳聚糖/聚乙烯醇复合膜性能与细胞相容性

doi:10.16085/j.issn.1000-6613.2019-0642

摘要/Abstract

摘要：

以椰壳纤维为原料，制备了纳米纤维素晶须，用硅烷偶联剂对纳米纤维素晶须进行改性，将改性后纳米纤维素晶须与壳聚糖、聚乙烯醇共混，采用溶液浇铸法制备了改性纳米纤维素晶须-壳聚糖/聚乙烯醇复合膜。采用FTIR、DSC、TG、XRD和SEM对改性纳米纤维素晶须-壳聚糖/聚乙烯醇复合膜的结构、热性能、结晶行为和形貌进行表征与分析，对复合膜的力学性能和水接触角进行测试，将成纤维细胞L929接种到复合膜上，对其进行细胞相容性实验。结果表明，添加改性纤维素晶须，能够使壳聚糖/聚乙烯醇复合膜的热性能、结晶行为和力学性能提高，成纤维细胞在复合膜上具有较好的黏附和生长，制备的纳米纤维素晶须-壳聚糖/聚乙烯醇复合膜具有良好的综合性能和细胞相容性。

关键词: 椰壳纤维, 壳聚糖, 聚乙烯醇, 复合材料, 细胞相容性

Abstract:

Nano-cellulose whiskers were prepared from coconut shell fibers as raw material. The modified nano-cellulose whiskers were modified by silane coupling agent and dispersed in the mixture of chitosan and polyvinyl alcohol. The modified nano-cellulose whiskers-chitosan/polyvinyl alcohol composite membranes were prepared by solution casting method. The structure, thermal properties, crystallization behavior and morphology of the modified nano-cellulose whisker-chitosan/polyvinyl alcohol composite membrane were characterized and analyzed by FTIR, DSC, TG, XRD and SEM. The mechanical properties and dynamic water contact angle of the composite membrane were tested. The in vitro biocompatibility of the composite membrane was evaluated in this study using L929 fibroblasts. The results showed that the thermal properties, crystallization behavior and mechanical properties of chitosan/polyvinyl alcohol composite membranes were improved by adding modified cellulose whiskers. Fibroblasts had better adhesion and growth on the composite membranes. The nano-cellulose whisker-chitosan/polyvinyl alcohol composite membranes had good comprehensive properties and cell compatibility.

Key words: coconut shell fiber, chitosan, polyvinyl alcohol, composites, anocellulose whisker, cell compatibility

中图分类号:

TQ317.9

李桂娟,夏伟,罗海希,程桂茹. 纳米纤维素晶须-壳聚糖/聚乙烯醇复合膜性能与细胞相容性[J]. 化工进展, 2020, 39(1): 356-364.

Guijuan LI,Wei XIA,Haixi LUO,Guiru CHENG. Properties and in vitro cell compatibility of NCW-CS/PVA composite membranes[J]. Chemical Industry and Engineering Progress, 2020, 39(1): 356-364.

图/表 13

图1 NCW在溶剂中的分散情况

图2 改性后纤维素晶须透射电镜照片

图3 改性前后纤维素晶须红外光谱图

图4 四种膜的红外光谱图

图5 四种膜的XRD曲线

图6 四种膜的DSC曲线

图7 四种膜的TG-DTG曲线

表1 复合膜的力学性能数据

项目	CS/PVA	MNCW-CS/PVA
拉伸强度/MPa	21.96	23.28
弹性模量/MPa	332.36	487.77
断裂伸长率/%	204.85	148.36

图8 四种膜与水接触角的图像

图9 CS/PVA复合膜、MNCW-CS/PVA复合膜扫描电镜照片

图10 CS/PVA复合膜、MNCW-CS/PVA复合膜原子力显微镜照片

图11 成纤维细胞L929在四种膜上的分布和表面细胞形态

图12 细胞L929在四种膜材料上的黏附、增殖与时间的关系

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