聚己内酯/壳聚糖核壳结构纤维引导组织再生膜的制备及表征

doi:10.16085/j.issn.1000-6613.2018-1200

化工进展 ›› 2019, Vol. 38 ›› Issue (03): 1501-1508.DOI: 10.16085/j.issn.1000-6613.2018-1200

聚己内酯/壳聚糖核壳结构纤维引导组织再生膜的制备及表征

邓丹¹(),李玉宝¹,黄金会¹,孙富华¹,左奕¹,李吉东¹(),王亚宁²()

1. 四川大学纳米生物材料研究中心，四川成都 610064
2. 四川大学国家生物医学材料工程技术研究中心，四川成都 610065

收稿日期:2018-06-08 修回日期:2018-08-06 出版日期:2019-03-05 发布日期:2019-03-05
通讯作者: 李吉东,王亚宁
作者简介:邓丹（1993—），女，硕士研究生，研究方向为生物医学材料。E-mail：496097059@qq.com。|李吉东，博士，教授，主要从事生物医学材料研究。E-mail：nic1979@scu.edu.cn|王亚宁，硕士，助理研究员。主要从事生物医学材料评价。E-mail:wangyaning@scu.edu.cn
基金资助:
国家重点研发计划(2016YFA0201703/2016YFA021700);国家自然科学基金(31670965)

Preparation and characterization of a guided tissue regeneration membrane constructed by core-shell polycaprolactone/chitosan fibers

Dan DENG¹(), LIＹubao¹,Jinhui HUANG¹,Fuhua SUN¹,Yi ZUO¹,Jidong LI¹(),Yaning WANG²()

1. The Research Center for Nano-Biomaterial, Sichuan University, Chengdu 610064, Sichuan, China
2. National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610065, Sichuan, China

Received:2018-06-08 Revised:2018-08-06 Online:2019-03-05 Published:2019-03-05
Contact: Jidong LI,Yaning WANG

摘要/Abstract

摘要：

高性能的引导组织再生膜是牙周引导组织再生术成功的关键，静电纺丝法因可仿生制备类细胞外基质结构，在引导组织再生膜研制方面显示出巨大潜力。本研究通过同轴静电纺丝法，以聚己内酯（PCL）为核层，壳聚糖（CS）为壳层，制备核壳结构的纳米纤维，并用香草醛对制备的纤维膜进行交联。利用扫描电子显微镜（SEM）、透射电子显微镜（TEM）、X 射线衍射（XRD）、傅里叶变换红外光谱（FTIR）、力学测试及细胞培养等手段对制备的纤维膜进行形貌、内部结构、化学组成、力学性能和细胞相容性表征。结构分析表明本研究成功制备了核壳结构的PCL-CS纤维膜。力学测试和亲疏水性测试结果表明交联后的纤维膜具有较好的耐水性和力学性能，断裂强度高出文献报道值近两倍；体外细胞培养结果显示MG-63细胞能在交联后的纤维膜上黏附和持续增殖，表明纤维膜具有较好的细胞相容性，在引导组织再生领域有较好的应用前景。

关键词: 聚己内酯, 壳聚糖, 核-壳结构, 纳米纤维, 引导组织再生膜

Abstract:

High-performance guided tissue regeneration (GTR) membrane is a key factor for successful GTR treatment. Electrospinning has great potential for construction of a GTR membrane because this technique can prepare fibrous structural membrane similar to extracellular matrix, which is beneficial for cell adhesion and proliferation. In this study, the core-shell nanofibers were prepared by coaxial electrospinning technique with polycaprolactone (PCL) as the core and chitosan (CS) as the shell, and the resulting membrane was crosslinked by vanillin. Furthermore, the morphology, internal structure, chemical composition, mechanical properties and cytocompatibility of the fabricated membrane were characterized by scanning electron microscope (SEM), transmission electron microscopy (TEM), X ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), mechanical testing and cell culture experiments. Structure analysis showed a core-shell PCL-CS nanofiber constructed membrane was successfully prepared. The results of mechanical and contact angle test showed that the crosslinked fiber membrane had better water resistance, higher mechanical properties and a tensile strength nearly two times higher than the reported studies. Cell culture results showed that the crosslinked fibrous membrane was a good candidate for MG-63 cell adhesion and proliferation, indicating the fabricated membrane has good cytocompatibility. In general, the fibrous GTR membrane constructed by core-shell PCL-CS nanofibers holds promising application.

Key words: polycaprolactone, chitosan, core-shell structure, nanofiber, guided tissue regeneration membrane

中图分类号:

O631.2

邓丹,李玉宝,黄金会,孙富华,左奕,李吉东,王亚宁. 聚己内酯/壳聚糖核壳结构纤维引导组织再生膜的制备及表征[J]. 化工进展, 2019, 38(03): 1501-1508.

Dan DENG, LIＹubao,Jinhui HUANG,Fuhua SUN,Yi ZUO,Jidong LI,Yaning WANG. Preparation and characterization of a guided tissue regeneration membrane constructed by core-shell polycaprolactone/chitosan fibers[J]. Chemical Industry and Engineering Progress, 2019, 38(03): 1501-1508.

图/表 9

图1 纤维的扫描电镜图

图2 纤维的透射电镜图片

图3 纤维的X射线衍射图谱

图4 纤维膜红外光谱图

图5 纤维膜的应力-应变曲线

表1 纤维的平均断裂伸长率与平均断裂强度

样品	平均断裂伸长率/%	平均断裂强度/MPa
PCL	103.136±17.853	10.294±2.142
未交联的PCL-CS	8.418±0.993	2.641±0.157
交联的PCL-CS	5.517±1.032	3.740±0.590

图6 纤维膜的接触角测试图

图7 MG-63细胞在交联后的PCL-CS纤维膜上的形态

图8 MG-63细胞在交联后的PCL-CS纤维膜上的增殖情况

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聚己内酯/壳聚糖核壳结构纤维引导组织再生膜的制备及表征

Preparation and characterization of a guided tissue regeneration membrane constructed by core-shell polycaprolactone/chitosan fibers

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