化工进展 ›› 2015, Vol. 34 ›› Issue (1): 278-284.DOI: 10.16085/j.issn.1000-6613.2015.01.049

• 资源与环境化工 • 上一篇    下一篇

聚合氯化铝投加时间对好氧颗粒污泥的形成和胞外聚合物的影响

王亚利, 刘永军, 刘喆, 程祯, 李星, 杨贺棋   

  1. 西安建筑科技大学环境与市政工程学院, 陕西 西安 710055
  • 收稿日期:2014-04-16 修回日期:2014-06-04 出版日期:2015-01-05 发布日期:2015-01-05
  • 通讯作者: 刘永军,教授,主要研究领域为污水生物处理理论与技术、污染环境的生物修复。E-mail liuyongjun@xauat.edu.cn。
  • 作者简介:王亚利(1989-),女,硕士,主要研究方向为污水生物处理理论与技术。
  • 基金资助:

    国家自然科学基金项目(51178377)。

Influence of poly aluminium chloride dosing time on formation and EPS of aerobic granular sludge

WANG Yali, LIU Yongjun, LIU Zhe, CHENG Zhen, LI Xing, YANG Heqi   

  1. School of Environment and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, Shaanxi, China
  • Received:2014-04-16 Revised:2014-06-04 Online:2015-01-05 Published:2015-01-05

摘要: 通过在好氧颗粒污泥培养过程中分别在第1~7天和第8~14天投加聚合氯化铝(PAC)的方式,分析颗粒的形成过程,并研究胞外聚合物总量及其各组分的含量变化和空间分布。研究发现:第8~14天投加PAC的反应器形成颗粒时间提前了6天,且形成的颗粒外形规则,大小均匀,去除污染物性能良好;反应器中胞外多聚物和蛋白质含量总体较多,且随颗粒的形成而增加,之后略有下降并在颗粒成熟后维持稳定,蛋白质/多糖的值在颗粒形成期均增加超过2.5倍,有利于颗粒污泥的形成;多重荧光染色表明:两个反应器中PAC投加时间不同,β-D-呋喃葡萄糖、脂类和活细胞均呈现不同的分布情况,但对蛋白质和α-呋喃葡萄糖、α-甘露糖分布的影响较小。

关键词: 好氧颗粒污泥, 胞外聚合物, 空间分布, 操作条件, 荧光染色

Abstract: This paper analyzed the formation process of granules, and researched the content changes and the spatial distribution of extracellular polymeric substances by adding poly aluminium chloride in 1—7 days and 8—14 days respectively during the process of cultivating aerobic granular sludge. The granules formation time of the reactor adding PAC in 8—14 days was 6 days ahead of time, and the sludge granules had regular shape, uniform size, and good contaminants removal performance. Moreover, the reactor extracellular polymeric substances and proteins content was higher, and increased with the formation of granules and then decreased slightly, and stayed stable after the granules matured. The value of proteins/polysaccharides during the process of granules formation increased by more than 2.5 times, which was conducive to the formation of granular sludge. Multiple fluorescent staining showed that different PAC dosing times between the reactors led to different distributions of β-polysaccharides and lipids and live cells, but had smaller effect on the distribution of proteins and α-polysaccharides .

Key words: aerobic granular sludge, extracellular polymeric substances, spatial distribution, operating conditions, fluorescent staining

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