膨胀颗粒污泥的恢复及其基质降解动力学

doi:10.16085/j.issn.1000-6613.2017-2379

摘要/Abstract

摘要： 为实现丝状菌膨胀的好氧颗粒污泥（AGS）的修复，通过在序批式活性污泥（sequencing batch reactor activated sludge process，SBR）反应器中设置厌氧生物选择段以抑制丝状菌生长，并研究了修复过程中污泥基质降解动力学参数变化。膨胀AGS表面包裹了大量丝状菌（污泥体积指数SVI高达186.56mL/g），但恢复过程中膨胀AGS的比例逐渐减少，表面逐渐变得清晰、规则，21d时污泥膨胀趋势已完全得到遏止，最终AGS的污泥沉降比SV₃₀/SV₅、SVI和颗粒化率分别为0.92、48.74mL/g及92.79%，并表现出良好的污染物去除效果。通过双倒数法拟合得到丝状菌膨胀AGS的饱和常数和最大比增长速率分别为75.67mg/L、0.47h^–1，该值略高于普通活性污泥，但远低于恢复稳定后AGS的354.47mg/L、1.43h^–1。因此，在厌氧生物选择段创造的高底物浓度环境下，AGS中菌胶团细胞可优先获得基质并实现增殖，而丝状菌由于生长受到抑制而逐渐被淘汰，最终在22d内实现膨胀AGS的修复。

关键词: 好氧颗粒污泥, 丝状菌膨胀, 动力学, 稳定性, 修复

Abstract: In order to realize the remediation of filamentous bulking aerobic granular sludge (AGS), an anaerobic biological selector was employed in a SBR to inhibit filamentous bacteria growth. Variations of sludge substrate degradation kinetic parameters during the remediation process were investigated. A large amount of filamentous bacteria were entrapped on the surface of the bulking AGS (SVI was up to 186.56mL/g). However, the proportion of bulking AGS gradually decreased and its surface gradually became smooth and clear, and the trend of sludge bulking was completely inhibited on the 21st day. SV₃₀/SV₅, SVI and granulation rate of the AGS eventually reached to 0.92, 48.74mL/g and 92.79% respectively, and good pollutants removal performances were achieved. The maximum specific growth rate and saturated constant of filamentous bulking AGS were obtained by double reciprocal method, which were 75.67mg/L and 0.47h^-1, respectively. Although the two parameters were slightly higher than that of activated sludge, they were much lower than that of AGS recovered, which were 354.47mg/L and 1.43h^-1.Therefore, the zoogloea bacteria insides AGS was prior to obtain substrate and proliferate under high substrate concentration environment created by the anaerobic biological selector, while the filamentous fungi gradually eliminated due to the growth inhibition, and remediation of bulking AGS was finally realized within 22 days.

Key words: aerobic granular sludge, filamentous bulking, kinetics, stability, remediation

中图分类号:

X703

宣鑫鹏, 张立楠, 赵珏, 王智勇, 程媛媛, 龙焙. 膨胀颗粒污泥的恢复及其基质降解动力学[J]. 化工进展, DOI: 10.16085/j.issn.1000-6613.2017-2379.

XUAN Xinpeng, ZHANG Linan, ZHAO Jue, WANG Zhiyong, CHENG Yuanyuan, LONG Bei. Remediation of filamentous bulking granule and its substrate degradation kinetics[J]. Chemical Industry and Engineering Progress, DOI: 10.16085/j.issn.1000-6613.2017-2379.

参考文献

[1] ADAV S S, LEE D J, SHOW K Y, et al. Aerobic granular sludge:recent advances[J]. Biotechnology Advances, 2008, 26(5):411-423.
[2] 季斌, 陈威, 樊杰, 等. 好氧颗粒污泥的微生物研究进展[J]. 科学通报, 2017, 62(23):2639-2648. JI B, CHEN W, FAN J, et al. Research progress on microbes in aerobic granular sludge (in Chinese)[J]. Chinese Science Bulletin, 2017, 62(23):2639-2648.
[3] ZHANG Q, HU J, LEE D J. Aerobic granular processes:current research trends[J]. Bioresource Technology, 2016, 210:74-80.
[4] SHOW K Y, LEE D J, TAY J H. Aerobic granulation:advances and challenges[J]. Applied Biochemistry & Biotechnology, 2012, 167(6):1622-1640.
[5] LIU Y, LIU Q S. Causes and control of filamentous growth in aerobic granular sludge sequencing batch reactors[J]. Biotechnology Advances, 2006, 24(1):115-127.
[6] 唐朝春, 简美鹏, 刘名, 等. 强化好氧颗粒污泥稳定性的研究进展[J]. 化工进展, 2013, 32(4):919-924. TANG C C, JIAN M P, LIU M, et al. Research advances in aerobic granule stability enhancement[J]. Chemical Industry and Engineering Progress, 2013, 32(4):919-924.
[7] 赵霞, 赵阳丽, 陈忠林, 等. 好氧颗粒污泥发生丝状菌污泥膨胀的控制措施[J]. 中国给水排水, 2012, 28(3):15-19. ZHAO X, ZHAO Y L, CHEN Z L, et al. Control of filamentous sludge bulking in aerobic granular sludge SBR process[J]. China Water & Wastewater, 2012, 28(3):15-19.
[8] 龙焙, 濮文虹, 杨昌柱, 等. 失稳好氧颗粒污泥在SBR中的修复研究[J]. 中国给水排水, 2015, 31(7):29-33, 38. LONG B, PU W H, YANG C Z, et al. Research on remediation of instable aerobic granular sludge in sequencing batch reactor[J]. China Water & Wastewater, 2015, 31(7):29-33, 38.
[9] 张著, 高大文, 袁向娟, 等. 营养物质缺乏引起的好氧颗粒污泥膨胀及其恢复[J]. 环境科学, 2012, 33(9):3197-3201. ZHANG Z, GAO D W, YUAN X J, et al. Aerobic granular sludge bulking due to the lack of nutrient and its recovery[J]. Environmental Science, 2012, 33(9):3197-3201.
[10] 纪树兰, 李建婷, 秦振平, 等. 丝状膨胀好氧颗粒污泥细菌组成及丝状膨胀的控制[J]. 北京工业大学学报, 2011, 37(10):1530-1535. JI S L, LI J T, QIN Z P, et al. Bacteria composition of aerobic granular sludge under filamentous bulking and control method of filamentous bulking[J]. Journal of Beijing University of Technology, 2011, 37(10):1530-1535.
[11] 韩红桂, 伍小龙, 王丽丹, 等. 丝状菌污泥膨胀简化机理模型[J]. 化工学报, 2013, 64(12):4641-4648. HAN H G, WU X L, WANG L D, et al. Simplified mechanistic model of filamentous bulking sludge[J]. CIESC Journal, 2013, 64(12):4641-4648.
[12] 苏雪莹, 付昆明. 丝状菌在污水处理中的控制与应用[J]. 水处理技术, 2015, 41(9):19-23. SU X Y, FU K M. Control and application of filamentous bacteria in wastewater treatment[J]. Technology of Water Treatment, 2015, 41(9):19-23.
[13] 马智博, 李志华, 杨成建, 等. 低氧污泥丝状菌膨胀的呼吸图谱特征分析[J]. 环境科学, 2017, 38(9):3801-3806. MA Z B, LI Z H, YANG C J, et al. Analysis of respirogram characteristics of filamentous bulking caused by low dissolved oxygen[J]. Environmental Science, 2017, 38(9):3801-3806.
[14] LONG B, YANG C Z, PU W H, et al. Rapid cultivation of aerobic granular sludge in a pilot scale sequencing batch reactor[J]. Bioresource Technology, 2014, 166(8):57-63.
[15] 龙焙, 濮文虹, 杨昌柱, 等. 不同生物选择段的SBR中好氧颗粒污泥的特性[J]. 中国给水排水, 2015, 31(5):16-21. LONG B, PU W H, YANG C Z, et al. Characteristics of aerobic granular sludge in SBRS with different biological selectors[J]. China Water & Wastewater, 2015, 31(5):16-21.
[16] 程媛媛, 赵珏, 王家辰, 等. 设置生物选择段的SBR中好氧颗粒污泥的培养[J]. 中国给水排水, 2016, 32(21):17-22, 27. CHENG Y Y, ZHAO J, WANG J C, et al. Cultivation of aerobic granular sludge in SBR equipped with biological selector[J]. China Water & Wastewater, 2016, 32(21):17-22, 27.
[17] LIU L, WANG Z, JIE Y, et al. Investigation on the formation and kinetics of glucose-fed aerobic granular sludge[J]. Enzyme & Microbial Technology, 2005, 36(4):487-491.
[18] 李善评, 赵玉晓, 乔鹏, 等. 好氧颗粒污泥的培养及基质降解和污泥生长动力学分析[J]. 山东大学学报(工学版), 2008, 38(3):95-98. LI S P, ZHAO Y X, QIAO P, et al. Cultivation of aerobic granular sludge and the kinetics of substrate degradation and biomass growth[J]. Journal of Shandong University (Engineering Science), 2008, 38(3):95-98.
[19] 高大文, 刘琳. 不同饥饿时间下稳态好氧颗粒污泥系统的特性和动力学分析[J]. 中国科学(化学), 2011, 41(1)::97-104. GAO D W, LIU L. The characteristics and kinetic behaviors of steady-state aerobic granules under different aerobic starvation[J]. Scientia Sinica (Chimica), 2011, 41(1):97-104.
[20] 王硕, 时文歆, 于水利. 低温好氧颗粒污泥培养及其基质降解动力学[J]. 哈尔滨工业大学学报, 2012, 44(6):11-15. WANG S, SHI W X, YU S L. Cultivation of aerobic granules and substances degradation kinetics at low temperature[J]. Journal of Harbin Institute of Technology, 2012, 44(6):11-15.
[21] AWANG N A, SHAABAN M G. Effect of reactor height/diameter ratio and organic loading rate on formation of aerobic granular sludge in sewage treatment[J]. International Biodeterioration & Biodegradation, 2016, 112:1-11.
[22] 龙焙, 程媛媛, 赵珏, 等. 好氧颗粒污泥处理化工废水的应用研究[J]. 中国给水排水, 2017, 33(5):26-32. LONG B, CHENG Y Y, ZHAO J, et al. Treatment of chemical wastewater by aerobic granular sludge[J]. China Water & Wastewater, 2017, 33(5):26-32.
[23] 国家环境保护总局. 水和废水监测分析方法[M]. 4版. 北京:中国环境科学出版社, 2006. State Environmental Protection Administration. Standard methods for water and wastewater analysis[M]. 4th Ed. Beijing:China Environmental Science Publishing House, 2006.
[24] OCHOA J C, COLPRIM J, PALACIOS B, et al. Active heterotrophic and autotrophic biomass distribution between fixed and suspended systems in a hybrid biological reactor[J]. Water Science and Technology, 2002, 46:397-404.
[25] WANG Z W, LI Y, LIU Y. Mechanism of calcium accumulation in acetate-fed aerobic granule[J]. Applied Microbiology and Biotechnology, 2007, 74(2):467-473.
[26] OTHMAN I, ANUAR A N, UJANG Z, et al. Livestock wastewater treatment using aerobic granular sludge[J]. Bioresource Technology, 2013, 133(2):630-634.
[27] 彭赵旭, 彭永臻, 苏欣颖, 等. 黏性膨胀下活性污泥的脱氮除磷特点研究[J]. 北京大学学报(自然科学版), 2010, 46(3):417-421. PENG Z X, PENG Y Z, SU X Y, et al. Investigation of the characteristics of nitrogen and phosphorus removal under viscosity sludge bulking[J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2010, 46(3):417-421.
[28] 梅哈古丽·艾尼瓦尔, 姚俊芹, 宋文娟, 等. 膨胀污泥的胞外聚合物组分及其三维荧光光谱分析[J]. 中国给水排水, 2017, 33(7):109-112. MEIHAGULI A, YAO J Q, SONG W J, et al. Components and three-dimension excitation-emission matrix fluorescence spectral characteristics of EPS from bulking sludge[J]. China Water & Wastewater, 2017, 33(7):109-112.
[29] 彭永臻, 郭建华. 活性污泥膨胀机理、成因及控制[M]. 北京:科学出版社, 2012. PENG Y Z, GUO J H. Mechanism, causes and control of activated sludge[M]. Beijing:Science Press, 2012.
[30] 张兰河, 伏向宇, 张万友, 等. 序批式活性污泥法工艺处理含盐废水动力学模型研究[J]. 环境污染与防治, 2010, 32(11):69-73. ZHANG L H, FU X Y, ZHANG W Y, et al. Kinetic study on salinity wastewater treatment in SBR[J]. Environmental Pollution & Control, 2010, 32(11):69-73.
[31] 张秀霞, 张剑杰, 单宝来. 耐盐活性污泥驯化及其动力学实验研究[J]. 环境工程学报, 2011, 5(10):2283-2286. ZHANG X X, ZHANG J J, SHAN B L. Experimental study on acclimation of salt-tolerant activated sludge and its kinetics[J]. Chinese Journal of Environmental Engineering, 2011, 5(10):2283-2286.
[32] 薛涛, 俞开昌, 关晶, 等. MBR污水处理工艺中活性污泥动力学参数测定[J]. 环境科学, 2011, 32(4):1027-1033. XUE T, YU K C, GUAN J, et al. Determination of kinetic parameters of activated sludge in an MBR wastewater treatment plant[J]. Environmental Science, 2011, 32(4):1027-1033.
[33] 侯爱月, 李军, 王昌稳, 等. 不同好氧颗粒污泥中微生物群落结构特点[J]. 中国环境科学, 2016, 36(4):1136-1144. HOU A Y, LI J, WANG C W, et al. Characteristics of microbial community structure in different aerobic granular sludge[J]. China Environmental Science, 2016, 36(4):1136-1144.