酶制剂浓缩方法研究进展

doi:10.16085/j.issn.1000-6613.2015.06.004

化工进展 ›› 2015, Vol. 34 ›› Issue (06): 1526-1531.DOI: 10.16085/j.issn.1000-6613.2015.06.004

酶制剂浓缩方法研究进展

白净¹, 黄会杰¹, 陈俊英¹, 常春¹, 方书起¹, 李洪亮¹, 张璐¹, 闫德冉²

1 郑州大学化工与能源学院, 河南郑州 450001;
2 车用生物燃料技术国家重点实验室, 河南南阳 473000

收稿日期:2014-10-10 修回日期:2014-12-22 出版日期:2015-06-05 发布日期:2015-06-05
通讯作者: 陈俊英,副教授,研究方向为可再生能源。E-mail:chenjy@zzu.edu.cn。
作者简介:白净(1975—),男,博士,硕士生导师,从事新能源研究工作。E-mail:baijing@zzu.edu.cn
基金资助:
国家自然科学基金(U1404519)、河南省科技攻关计划(132102310042)及车用生物燃料技术国家重点实验室开放基金(KFKT2013012)项目。

Progress of concentrating enzyme

BAI Jing¹, HUANG Huijie¹, CHEN Junying¹, CHANG Chun¹, FANG Shuqi¹, LI Hongliang¹, ZHANG Lu¹, YAN Deran²

1 School of Chemical Engineering and Energy, Zhengzhou University, Zhengzhou 450001, Henan, China;
2 State Key Laboratory of Motor Vehicle Biofuel Technology, Nanyang 473000, Henan, China

Received:2014-10-10 Revised:2014-12-22 Online:2015-06-05 Published:2015-06-05

摘要/Abstract

摘要： 目前酶制剂生产过程中常用的浓缩方法存在工艺复杂、能耗较高、成本高昂等缺点, 并成为酶制剂工业进一步发展的关键步骤。本文综述了蒸发、超滤、吸附和冷冻等常用的酶制剂浓缩方法, 以及较新的离子液体和水合物浓缩酶制剂方法等, 并比较了这些方法的优缺点。蒸发浓缩酶制剂技术成熟, 但是能耗较高、酶失活率高。超滤和吸附浓缩酶制剂方法能耗较低, 但是膜和吸附材料成本较高、再生困难。冷冻酶制剂浓缩方法酶失活少, 但是浓缩率低。离子液体酶制剂浓缩方法具有保持酶活性、易于放大、可连续操作等优点, 但是技术仍不成熟。水合物酶制剂浓缩方法目前尚处于实验室研究阶段。因此, 开发出工艺简单、性能可靠、能耗较低、成本低廉的高纯度酶制剂浓缩方法依然是未来的研究方向。

关键词: 酶制剂, 浓缩, 分离, 进展

Abstract: Presently, the enzyme concentrating method as the critical procedure in production of enzyme has many disadvantages, including complex process and high energy consumption. The common methods, including evaporation, ultrafiltration, adsorption, freezing are compared with the latest methods, including ionic liquid, hydrate, and their advantages and disadvantages are analyzed. Evaporation technology is mature but consumes excessive energy and keeps less enzyme activity. Ultrafiltration and adsorption consume less energy but have high material costs and are difficult to regenerate. The disadvantage of freezing concentration is the limit of concentration, though it can keep more activity. Concentrating by the ionic liquid method is easy to scale up and has the advantage of continuous operation, but the technology is not mature enough. The hydrate method is still in the laboratory. So developing a method of simple process, reliable performance, low energy consumption and low cost will be a research direction in the future.

Key words: enzymes, concentrate, separation, progress

中图分类号:

TQ925

白净, 黄会杰, 陈俊英, 常春, 方书起, 李洪亮, 张璐, 闫德冉. 酶制剂浓缩方法研究进展[J]. 化工进展, 2015, 34(06): 1526-1531.

BAI Jing, HUANG Huijie, CHEN Junying, CHANG Chun, FANG Shuqi, LI Hongliang, ZHANG Lu, YAN Deran. Progress of concentrating enzyme[J]. Chemical Industry and Engineering Progree, 2015, 34(06): 1526-1531.

参考文献

[1] Liese A, Hilterhaus L. Evaluation of immobilized enzymes for industrial applications[J]. Chemical Society Reviews, 2013, 42(15):6236-6249.
[2] Kirk O, Borchert T V, Fuglsang C C. Industrial enzyme applications[J]. Current Opinion in Biotechnology, 2002, 13(4):345-351.
[3] Uhlig Helmut.Industrial Enzymes and Their Applications[M]. New York:John Wiley & Sons, 1998.
[4] 张伟, 詹志春. 饲用酶制剂研究进展与发展趋势[J]. 饲料工业, 2011(s1):11-19.
[5] 段钢. 新型工业酶制剂的进步对生物化学品工业生产过程的影响[J]. 生物工程学报, 2009, 25(12):1808-1818.
[6] Underkofler L A, Barton R R, Rennert S S. Production of microbial enzymes and their applications[J]. Applied Microbiology, 1958, 6(3):212.
[7] 严福红, 许喜林, 周凯翔. 碱性纤维素酶浓缩工艺的设计与应用[J]. 现代化工, 2007, 27(4):52-54.
[8] Cooney C L, Demain A L, Dunnill P, et al. Fermentation and Enzyme Technology[M]. New York:John Wiley & Sons, 1979.
[9] Ding Fengping, Noritomi H, Nagahama K. Concentration of alkaline pecticlyase with ultrafiltration process[J]. Membrane Science and Technology, 2001, 21(6):53-58.
[10] 刘宏波, 杨昌柱, 濮文虹, 等. 中空超滤在酶纯化浓缩中的应用[J]. 膜科学与技术, 2006, 26(5):81-85.
[11] 朱乐平, 杨娟, 高志明, 等. 喷射蒸煮结合超滤技术制备大豆分离蛋白及其表征[J]. 中国粮油学报, 2014, 29(9):29-33.
[12] 张建友, 柳敏, 林龙, 等. 鳀鱼蒸煮液膜浓缩与蒸发浓缩的比较分析[J]. 现代食品科技, 2014, 30(4):205-210.
[13] D'Alvise N, Lesueur-Lambert C, Fertin B, et al. Hydrolysis and large scale ultrafiltration study of alfalfa protein concentrate enzymatic hydrolysate[J]. Enzyme and Microbial Technology, 2000, 27(3):286-294.
[14] 杭华, 赵萌, 周榴明, 等. 超滤法浓缩菊糖果糖转移酶的初步研究[J]. 食品工业科技, 2011, 32(6):201-203.
[15] Eslie W D, Cheryan M. Continuous enzymatic modification of proteins in an ultrafiltration reactor[J]. Journal of Food Science, 1981, 46(4):1035-1042.
[16] 余旭聪. 微波真空浓缩设备的设计极其在酶溶液与食品浓缩中的应用研究[D]. 广州:华南理工大学, 2013.
[17] 杨宗正, 刘振义. 异形竖板降膜蒸发器浓缩耐温淀粉酶溶液的传热性能[J]. 食品与机械, 2001(1):15-17.
[18] 邹龙生, 陈德珍, 尹丽洁, 等. 稠油废水高倍蒸发浓缩污垢的形成和模拟[J]. 同济大学学报:自然科学版, 2014, 42(3):21-23.
[19] 施建杭, 陈小洁, 陈水超, 等. 太阳能集热真空膜蒸发系统及其盐溶液浓缩试验[J]. 膜科学与技术, 2014, 34(4):56-60.
[20] Huige N J J, Thijssen H A C. Production of large crystals by continuous ripening in a stirred tank[J]. Crystal Growth, 1972, 13-14:483-487.
[21] 陈锦权, 李彦杰, 孙沈鲁, 等. 高压脉冲电场结合冷冻浓缩生产浓缩绿茶汤工艺优化[J]. 农业工程学报, 2014, 30(2):260-268.
[22] Rane M V, Jabade S K. Freeze concentration of sugarcane juice in a jaggery making process[J]. Applied Thermal Engineering, 2005, 25(14):2122-2137.
[23] Flodin P, Gelotte B. A method for concentrating solutes of high molecular weight[J]. Nature, 1960, 188:493-494.
[24] Gehrke S H, Andrews G P, Cussler E L. Chemical aspects of gel extraction[J]. Chemical Engineering Science, 1986, 41(8):2153-2160.
[25] 王锦堂, 仲慧, 朱红军, 等. 温敏性水凝胶对蛋白质和酶浓缩分离性能[J]. 南京化工大学学报, 1998, 20(2):75-77.
[26] 黄健, 黄志明, 包永忠, 等. 表面强化交联聚(N-异丙基丙烯酰胺)水凝胶的温敏和浓缩分离性能[J]. 化工学报, 2006, 57(4):948-952.
[27] 李汝雄, 王建基. 绿色溶剂离子液体的制备与应用[J]. 化工进展, 2002, 21(1):43-48.
[28] Zhang S, Zhang Q, Zhang Z C. Extractive desulfurization and denitrogenation of fuels using ionic liquids[J]. Industrial & Engineering Chemistry Research, 2004, 43(2):614-622.
[29] Izák P, Köckerling M, Kragl U. Stability and selectivity of a multiphase membrane, consisting of dimethylpolysiloxane on an ionic liquid, used in the separation of solutes from aqueous mixtures by pervaporation[J]. Green Chemistry, 2006, 8(11):947-948.
[30] Pei Y, Wang J, Wu K, et al. Ionic liquid-based aqueous two-phase extraction of selected proteins[J]. Separation and Purification Technology, 2009, 64(3):288-295.
[31] Ruiz-Angel M J, Pino V, Carda-Broch S, et al. Solvent systems for countercurrent chromatography:An aqueous two phase liquid system based on a room temperature ionic liquid[J]. Journal of Chromatography A, 2007, 1151(1):65-73.
[32] 王军, 张艳, 时召俊, 等. N-乙基-N-丁基吗啉离子液体双水相体系萃取分离蛋白质[J]. 应用化工, 2009, 28(1):70-72.
[33] Cao Q, Quan L, He C, et al. Partition of horseradish peroxidase with maintained activity in aqueous biphasic system based on ionic liquid[J]. Talanta, 2008, 77(1):160-165.
[34] Shimojo K, Nakashima K, Kamiya N, et al. Crown ether-mediated extraction and functional conversion of cytochrome c in ionic liquids[J]. Biomacromolecules, 2006, 7(1):2-5.
[35] 邓凡政, 郭东方. 离子液体双水相体系萃取分离牛血清白蛋白[J]. 分析化学, 2006, 34(10):1451-1453.
[36] Du Z, Yu Y L, Wang J H. Extraction of proteins from biological fluids by use of an ionic liquid/aqueous two-phase system[J]. Chemistry:A European Journal, 2007, 13(7):2130-2137.
[37] Lund D B, Fennema O, Powrie W D. Effect of gas hydrates and hydrate formers on invertase activity[J]. Archives of Biochemistry and Biophysics, 1969, 129(1):181-188.
[38] Phillips J B, Huyen N, John V T. Protein recovery from reversed micellar solutions through contact with a pressurized gas phase[J]. Biotechnology Progress, 1991, 7(1):43-48.

酶制剂浓缩方法研究进展

Progress of concentrating enzyme

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	贺美晋. 分子管理在炼油领域分离技术中的应用和发展趋势[J]. 化工进展, 2023, 42(S1): 260-266.
[2]	崔守成, 徐洪波, 彭楠. 两种MOFs材料用于O₂/He吸附分离的模拟分析[J]. 化工进展, 2023, 42(S1): 382-390.
[3]	李世霖, 胡景泽, 王毅霖, 王庆吉, 邵磊. 电渗析分离提取高值组分的研究进展[J]. 化工进展, 2023, 42(S1): 420-429.
[4]	郭强, 赵文凯, 肖永厚. 增强流体扰动强化变压吸附甲硫醚/氮气分离的数值模拟[J]. 化工进展, 2023, 42(S1): 64-72.
[5]	廖志新, 罗涛, 王红, 孔佳骏, 申海平, 管翠诗, 王翠红, 佘玉成. 溶剂脱沥青技术应用与进展[J]. 化工进展, 2023, 42(9): 4573-4586.
[6]	潘宜昌, 周荣飞, 邢卫红. 高效分离同碳数烃的先进微孔膜：现状与挑战[J]. 化工进展, 2023, 42(8): 3926-3942.
[7]	周龙大, 赵立新, 徐保蕊, 张爽, 刘琳. 电场-旋流耦合强化多相介质分离研究进展[J]. 化工进展, 2023, 42(7): 3443-3456.
[8]	陈香李, 李倩倩, 张甜, 李彪, 李康康. 自愈合油水分离膜的研究进展[J]. 化工进展, 2023, 42(7): 3600-3610.
[9]	娄宝辉, 吴贤豪, 张驰, 陈臻, 冯向东. 纳米流体用于二氧化碳吸收分离研究进展[J]. 化工进展, 2023, 42(7): 3802-3815.
[10]	周磊, 孙晓岩, 陶少辉, 陈玉石, 项曙光. 基于分离因数法的简捷炼油塔模型开发及应用[J]. 化工进展, 2023, 42(6): 2819-2827.
[11]	吴和平, 曹宁, 徐圆圆, 曹云波, 李裕东, 杨强, 卢浩. 氢氟酸与烷基化油快速分离[J]. 化工进展, 2023, 42(6): 2845-2853.
[12]	孙鲁芹, 卢会霞, 王建友. 电渗析/超滤内在耦合过程分离蛋清中溶菌酶[J]. 化工进展, 2023, 42(5): 2262-2271.
[13]	宋民航, 赵立新, 徐保蕊, 刘琳, 张爽. 基于入口分散相重排序的旋流强化分离研究进展[J]. 化工进展, 2023, 42(5): 2219-2232.
[14]	赵尧, 周志辉, 吴红丹, 胡传智, 张国春, 吴睿鹏. Silicalite-1分子筛膜渗透蒸发条件的响应面分析与优化[J]. 化工进展, 2023, 42(5): 2586-2594.
[15]	庞楠炯, 王晓玲, 廖学品, 石碧. 胶原纤维固化黑荆树单宁对硼同位素的分离[J]. 化工进展, 2023, 42(5): 2616-2625.