严格厌氧发酵体系在线尾气监测系统的设计及在梭菌生产生物能源丁醇的应用

doi:10.16085/j.issn.1000-6613.2016.10.039

化工进展 ›› 2016, Vol. 35 ›› Issue (10): 3295-3300.DOI: 10.16085/j.issn.1000-6613.2016.10.039

严格厌氧发酵体系在线尾气监测系统的设计及在梭菌生产生物能源丁醇的应用

赵倩倩, 杜广庆, 陈丽杰, 薛闯, 白凤武

大连理工大学生命科学与技术学院, 辽宁大连 116023

收稿日期:2016-03-15 修回日期:2016-06-01 出版日期:2016-10-05 发布日期:2016-10-05
通讯作者: 薛闯,副教授,主要从事生物能源方面研究。E-mail:xue.1@dlut.edu.cn
作者简介:赵倩倩(1990-),女,硕士研究生。E-mail:519241612@qq.com。
基金资助:
国家自然科学基金（21576045,21306020）及辽宁省博士科研启动基金（20141197）项目。

The design of online off-gas analysis for anaerobic fermentation and its application in biobutanol production by Clostridium as advanced biofuels

ZHAO Qianqian, DU Guangqing, CHEN Lijie, XUE Chuang, BAI Fengwu

School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116023, Liaoning, China

Received:2016-03-15 Revised:2016-06-01 Online:2016-10-05 Published:2016-10-05

摘要/Abstract

摘要： 目前关于有氧或微氧的乙醇发酵的尾气检测系统已经建立，但是对于丙酮丁醇梭菌等严格厌氧菌发酵尾气的在线监测鲜见文献报道。因此，本研究以丙酮丁醇梭菌生产丁醇的严格厌氧发酵体系为研究对象，研究了传统有氧或微氧尾气检测方法在丙酮丁醇梭菌厌氧发酵体系中的检测结果，设计并建立了适合厌氧菌发酵的尾气在线监测系统，对丙酮丁醇梭菌的发酵过程实时监测。在建立有效的厌氧发酵尾气分析系统的条件下，比较了丙酮丁醇梭菌在游离细胞条件下和高细胞密度条件下的发酵性能。相比较于游离发酵，高细胞密度发酵的发酵周期明显缩短，丁醇生产强度显著提高。丁醇最高产量为15.4g/L，生产强度0.64g/（L·h），分别比对照组提高了12.4%和106%，CO₂和H₂最高产气速率分别提高60%和9%，产气量分别提高了20.7%和41.3%。尾气分析系统采集的气体数据为丙酮丁醇梭菌的代谢分析提供了重要依据。

关键词: 尾气在线监测, 厌氧发酵, 高密度细胞发酵, 丁醇, 生物燃料

Abstract: Currently,an online off-gas analysis for aerobic or micro-aerobic ethanol fermentation has been established,but the online off-gas analysis for Clostridium acetobutylicum and other strict anaerobic fermentation are rarely reported. Therefore,the study on anaerobic fermentation system is based on an online off-gas analysis for aerobic or micro-aerobic ethanol fermentation,and we take a strictly anaerobic fermentation system of C. acetobutylicum which produces butanol as our research target. Then we get a detection result for the C. acetobutylicum fermentation system by traditional aerobic or micro oxygen gas. Then a design of online off-gas analysis for anaerobic fermentation can be confirmed. Using the online off-gas analysis,the fermentation performance of C.acetobutylicum under the condition of high cell density was investigated. Compared with the batch fermentation,the fermentation time was shortened,and thus butanol productivity was markedly increased. C.acetobutylicum produced 15.40g/L butanol with a productivity of 0.64g/(L·h) when grown in high cell density. Meanwhile,the butanol production and the productivity were 12.4% and 106%,respectively,higher than those of the control. CO₂ and H₂ maximum gas rate increased by 60% and 9% respectively,while gas production increased by 20.7% and 41.3%,respectively,while gas data from online off-gas analysis provides an important basis for metabolic analysis of C.acetobutylicum.

Key words: online off-gas analysis, anaerobic fermentation, high cell density fermentation, butanol, biofuel

中图分类号:

TQ923

赵倩倩, 杜广庆, 陈丽杰, 薛闯, 白凤武. 严格厌氧发酵体系在线尾气监测系统的设计及在梭菌生产生物能源丁醇的应用[J]. 化工进展, 2016, 35(10): 3295-3300.

ZHAO Qianqian, DU Guangqing, CHEN Lijie, XUE Chuang, BAI Fengwu. The design of online off-gas analysis for anaerobic fermentation and its application in biobutanol production by Clostridium as advanced biofuels[J]. Chemical Industry and Engineering Progree, 2016, 35(10): 3295-3300.

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严格厌氧发酵体系在线尾气监测系统的设计及在梭菌生产生物能源丁醇的应用

The design of online off-gas analysis for anaerobic fermentation and its application in biobutanol production by Clostridium as advanced biofuels

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