碱联合超高压预处理对笋壳酶解效率的影响

doi:10.16085/j.issn.1000-6613.2021-2111

化工进展 ›› 2022, Vol. 41 ›› Issue (3): 1357-1363.DOI: 10.16085/j.issn.1000-6613.2021-2111

• 可再生能源的开发利用 • 上一篇下一篇

碱联合超高压预处理对笋壳酶解效率的影响

王延云¹^,²(), 胡强¹^,²(), 龚卫华³, 王燕¹^,², 吴蔚⁴

^1.乐山师范学院竹类病虫防控与资源开发四川省重点实验室，四川乐山 614000
^2.乐山师范学院生命科学学院，四川乐山 614000
^3.吉首大学杜仲综合利用技术国家地方联合工程实验室，湖南吉首 416000
^4.乐山市产品质量监督检验所，四川乐山 614000

收稿日期:2021-10-12 修回日期:2021-12-22 出版日期:2022-03-23 发布日期:2022-03-28
通讯作者: 胡强
作者简介:王延云（1979—），女，硕士，讲师，研究方向为竹资源开发与利用。E-mail：40516665@qq.com。
基金资助:
乐山市科技局重点研究项目(21NZD044)

Effects of alkaline combined with ultra-high pressure pretreatment on enzymatic hydrolysis efficiency of bamboo shell

WANG Yanyun¹^,²(), HU Qiang¹^,²(), GONG Weihua³, WANG Yan¹^,², WU Wei⁴

^1.Bamboo Diseases and Pests Control and Resources Development Key Laboratory of Sichuan Province, Leshan Normal University, Leshan 614000, Sichuan, China
^2.College of Life Science, Leshan Normal University, Leshan 614000, Sichuan, China
^3.Jishou University National and Local United Engineering Laboratory of Integrative Utilization Technology of Eucommia Ulmoides, Jishou University, Jishou 416000, Hunan, China
^4.Leshan Product Quality Supervision and Testing Institute, Leshan 614000, Sichuan, China

Received:2021-10-12 Revised:2021-12-22 Online:2022-03-23 Published:2022-03-28
Contact: HU Qiang

摘要/Abstract

摘要：

以竹笋壳（BS）为原料，采用单独超高压（UHP）、单独碱加热（AH）、超高压后碱加热（UHP+AT）和碱加热后超高压（AT+UHP）4种方法进行预处理，并对比分析了样品预处理前后的化学组成、扫描电镜（SEM）、晶体结构（XRD）、红外光谱（FTIR）、比表面积和孔结构及酶水解效率的变化规律。结果表明：与其他处理方法相比，AT+UHP在450MPa压强下处理效果最好。预处理后样品中木质素的脱除率为86.87%，微观表面结构松散和粗糙，结晶指数有所上升，比表面积和孔体积分别为2.590m²/g、0.010cm³/g，酶水解效率高达97.89%。UHP+AT与AT+UHP预处理效果差异不明显，其酶解效率达到96.94%。因此，碱联合超高压处理是生物燃料生产中生物质预处理的一种潜在选择。

关键词: 超高压, 笋壳, 生物质, 酶, 水解

Abstract:

Bamboo shell (BS) was pretreated by ultra-high pressure (UHP), alkaline-heating method (AH), UHP combined with AH (UHP+AH) and AH combined with UHP (AH+UHP). The chemical composition was determined. And scanning electron microscopy (SEM), crystal structure (XRD), infrared spectroscopy (FTIR), specific surface area and pore structure, and enzymatic hydrolysis were used to analyze the changes of samples before and after pretreatment. The results showed that AT+UHP pretreatment had the best effect under 450MPa pressure compared with other pretreatment methods. After pretreatment with AT+UHP at 450MPa, 86.87% of the lignin was removed, the surface was loose and coarse, and the crystallization index increased, the specific surface area and pore size were 2.590m²/g and 0.010cm³/g, respectively, while the enzymatic hydrolysis efficiency was 97.89%. There was no obvious difference between the pretreatment effects of UHP+AT and AT+UHP. The enzymatic hydrolysis efficiency of UHP+AT pretreatment sample was up to 96.94%. Therefore, alkaline combined with ultra-high pressure pretreatment can be a potential alternative for biomass pretreatment in biofuel production.

Key words: ultra-high pressure, bamboo shell, biomass, enzyme, hydrolysis

中图分类号:

TQ353.1

王延云, 胡强, 龚卫华, 王燕, 吴蔚. 碱联合超高压预处理对笋壳酶解效率的影响[J]. 化工进展, 2022, 41(3): 1357-1363.

WANG Yanyun, HU Qiang, GONG Weihua, WANG Yan, WU Wei. Effects of alkaline combined with ultra-high pressure pretreatment on enzymatic hydrolysis efficiency of bamboo shell[J]. Chemical Industry and Engineering Progress, 2022, 41(3): 1357-1363.

图/表 6

参考文献 33

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处理方式	压力/MPa	纤维素/%	半纤维素/%	AIL/%
未处理	0	34.42±0.67	23.07±0.37	19.58±0.24
UHP	150	41.85±0.52	20.42±0.35	15.50±0.22
	300	40.94±0.65	21.01±0.76	15.74±0.14
	450	41.23±0.82	21.02±0.68	15.06±0.20
	600	40.27±0.65	20.47±0.55	15.02±0.24
AH	0	59.57±0.72	18.84±0.24	2.85±0.15
UHP+AT	150	59.42±1.09	16.34±0.15	3.59±0.08
	300	59.92±0.55	16.47±0.44	3.47±0.11
	450	59.09±1.07	16.57±0.43	3.79±0.10
	600	60.08±0.92	16.23±0.40	3.88±0.09
AT+UHP	150	59.35±0.95	15.48±0.50	2.59±0.10
	300	59.63±1.20	15.93±0.32	2.70±0.15
	450	59.58±1.29	16.00±0.47	2.57±0.15
	600	60.39±0.96	16.04±0.65	3.50±0.11

处理方式	压力/MPa	纤维素/%	半纤维素/%	AIL/%
未处理	0	34.42±0.67	23.07±0.37	19.58±0.24
UHP	150	41.85±0.52	20.42±0.35	15.50±0.22
	300	40.94±0.65	21.01±0.76	15.74±0.14
	450	41.23±0.82	21.02±0.68	15.06±0.20
	600	40.27±0.65	20.47±0.55	15.02±0.24
AH	0	59.57±0.72	18.84±0.24	2.85±0.15
UHP+AT	150	59.42±1.09	16.34±0.15	3.59±0.08
	300	59.92±0.55	16.47±0.44	3.47±0.11
	450	59.09±1.07	16.57±0.43	3.79±0.10
	600	60.08±0.92	16.23±0.40	3.88±0.09
AT+UHP	150	59.35±0.95	15.48±0.50	2.59±0.10
	300	59.63±1.20	15.93±0.32	2.70±0.15
	450	59.58±1.29	16.00±0.47	2.57±0.15
	600	60.39±0.96	16.04±0.65	3.50±0.11

处理方式	比表面积/m2·g^-1	孔体积/cm3·g^-1
未处理	0.821	0.003
UHP	0.892	0.004
AH	1.385	0.006
UHP+AT	2.071	0.007
AT+UHP	2.590	0.010

处理方式	比表面积/m2·g^-1	孔体积/cm3·g^-1
未处理	0.821	0.003
UHP	0.892	0.004
AH	1.385	0.006
UHP+AT	2.071	0.007
AT+UHP	2.590	0.010

处理方式	压力值/MPa	酶水解率/%
未处理	0	13.51±1.42
UHP	150	49.13±2.19
	300	53.44±2.56
	450	53.74±1.29
	600	51.67±1.46
AH	0	75.35±3.65
UPH+AT	150	96.82±5.44
	300	96.71±4.12
	450	96.94±5.68
	600	95.33±4.53
AT+UHP	150	94.52±4.89
	300	96.73±5.22
	450	97.89±2.45
	600	95.31±3.12

碱联合超高压预处理对笋壳酶解效率的影响

Effects of alkaline combined with ultra-high pressure pretreatment on enzymatic hydrolysis efficiency of bamboo shell

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