关键词: 头孢菌素C, 综纤维素, 糖化液, 顶头孢霉, 发酵

Abstract: The cephalosporin C (CPC) and other antibiotics fermentation face the foodstuff competition problem with human beings, as the carbon sources derived from corn, wheat and sweet potato starch. It is a beneficial attempt to deal with this problem by using abundant lignocellulosic biomass as the substitution of starch. Rice straw holocellulose hydrolyte was used as available carbon source and the mixture of holocellulose and cellulase was continuous carbon source in fermentation medium. While holocellulose content, cellulase dosage, (NH₄)₂SO₄ amount, corn syrup addition and the initial pH were 40g/L, 0.4%, 10g/L, 60g/L and 6.0, respectively, the fermentation of Cephalosporium acremonium CPCC 400039 attained the maximum CPC yield, 382.12U/mL, 84.04% of the yield in the standard medium. Also, this study further confirmed that rice straw pretreated with SO₃ micro-thermal explosion could be utilized as an carbon source alternative to the cephalosporin C and other antibiotics fermentation as well as other industrial fermentation, due to the high glucose content and the negligible content of furfural and other by-products in holocellulose hydrolyte of the pretreated rice straw.

Key words: cephalosporin C, holocellulose, hydrolyte, Cephalosporium acremomium, fermentation