磁性核苷酸基MOFzyme的构建及检测肾上腺素

doi:10.16085/j.issn.1000-6613.2016.s2.052

化工进展 ›› 2016, Vol. 35 ›› Issue (S2): 301-304.DOI: 10.16085/j.issn.1000-6613.2016.s2.052

磁性核苷酸基MOFzyme的构建及检测肾上腺素

林菲菲, 梁浩

北京化工大学生命科学与技术学院制药工程系, 北京 100029

收稿日期:2016-09-26 出版日期:2016-12-31 发布日期:2016-12-22
通讯作者: 梁浩,博士,教授。E-mail:lianghao@mail.buct.edu.cn。
作者简介:林菲菲(1991-),女,硕士研究生。
基金资助:
国家自然科学基金面上项目（81373284）。

Construction of magnetic nucleotide-base MOFzyme applied to the detection of adrenaline

LIN Feifei, LIANG Hao

Beijing University of Chemical Technology, College of Life Science and Technology, Pharmaceutical Engineering, Beijing 100029

Received:2016-09-26 Online:2016-12-31 Published:2016-12-22

摘要/Abstract

摘要： 漆酶是一类含铜多酚氧化酶，能够催化空气中的氧气直接氧化各种酚类、芳胺类化合物。但由于漆酶稳定性差且无法重复利用限制其应用，本研究中利用鸟嘌呤核苷酸与Cu²⁺配位自组装并与Fe₃O₄形成具有磁性的Fe₃O₄@Cu/GMP复合物，可催化氧化酚类物质，并与4-氨基安替吡啉发生偶联反应得到红色产物，在510nm处有紫外吸收，由此说明其具有模拟漆酶活性。通过Fe₃O₄@Cu/GMP CPs与漆酶在不同的pH、温度以及储存稳定性的研究，表明Fe₃O₄@Cu/GMP CPs具有明显优于漆酶的稳定性。通过重复利用的实验表明Fe₃O₄@Cu/GMP CPs重复利用5次后活性还能保持在70%以上。比较Fe₃O₄@Cu/GMP CPs和游离漆酶应用于肾上腺素的检测效果，实验表明Fe₃O₄@Cu/GMP CPs对肾上腺素的催化性能显著优于漆酶，其检测限为0.375μg/mL。

关键词: 漆酶, 金属有机骨架, 磁性, 鸟嘌呤核苷酸, 二价铜

Abstract: Laccase is a kind of copper-containing polyphenol oxidase,and can catalyze oxidation of various phenolic and aromatic amines by the oxygen in the air directly.But the poor stability and the impossibility for reuse limit the application of laccase.Fe₃O₄@Cu/GMP complexes formed by coordination self-assembly with magnetic properties using Fe₃O₄ and guanine nucleotide and Cu²⁺ in this study,can catalyze the oxidation of phenols and 4-amino antipyrine to produce red product with ultraviolet absorption in 510nm.Therefore,it has simulated laccase activity.Compared Fe₃O₄@Cu/GMP CPs with laccase in different pH,temperature and studied their storage stability,the results showed that the stability of Fe₃O₄@Cu/GMP CPs is superior to laccase.The experiment of the repeated use showed that the activity of Fe₃O₄@Cu/GMP CPs still remained above 70% after 5 times reuse.The application to the detection of adrenaline showed that the catalytic performance of Fe₃O₄@Cu/GMP CPs was significantly better than that of laccase.The detection limit of Fe₃O₄@Cu/GMP CPs is 0.375g/mL.

Key words: laccase, metal organic framework, magnetic, guanine nucleotides, Cu²⁺

中图分类号:

TQ467

林菲菲, 梁浩. 磁性核苷酸基MOFzyme的构建及检测肾上腺素[J]. 化工进展, 2016, 35(S2): 301-304.

LIN Feifei, LIANG Hao. Construction of magnetic nucleotide-base MOFzyme applied to the detection of adrenaline[J]. Chemical Industry and Engineering Progree, 2016, 35(S2): 301-304.

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磁性核苷酸基MOFzyme的构建及检测肾上腺素

Construction of magnetic nucleotide-base MOFzyme applied to the detection of adrenaline

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