关键词: 丁二酸, 发酵, 纤维二糖, 纤维素水解液, Actinobacillus succinogenes NJ113

Abstract: Cellobiose was often found in cellulose hydrolysates. In this study，the ability to use cellobiose to produce succinic acid by A.succinogenes NJ113 and using cellobiose prepared from sugarcane bagasse cellulose hydrolysates as a carbon source to produce succinic acid were investigated. A final succinic acid concentration of 23.51 g/L with a yield of 67.17% was achieved from an initial cellobiose concentration of 35 g/L via batch fermentation. In batch fermentation with 18 g/L of cellobiose and 17 g/L of other sugars from sugarcane bagasse cellulose hydrolysates，a succinic acid concentration of 20.00 g/L was obtained，with a yield of 64.73%. This study suggested that A. succinogenes had a strong ability to convert cellobiose into succinic acid and cellobiose from cellulose hydrolysate could be a potential carbon source for economical and efficient succinic acid production by A. succinogenes.

Key words: succinic acid, fermentation, cellobiose, cellulose hydrolysate, Actinobacillus succinogenes NJ113