降解偶氮染料嗜盐菌的分离、降解特性及机制

doi:10.16085/j.issn.1000-6613.2022-1092

摘要/Abstract

摘要：

高盐限制了普通微生物处理印染废水的效果，分离嗜盐微生物对于提高高盐印染废水的处理效率，具有重要的应用价值。本研究从印染废水的活性污泥中，分离了一株降解酸性金黄G的菌株，通过16S rDNA对该菌进行鉴定，并研究了其降解机理。结果表明，该菌与Exiguobaterium strain ACCC11618同源性最高，属于微小杆菌属。该菌在5%盐度下，8h内对100mg/L的酸性金黄G脱色95%以上。最佳脱色条件是30℃下，pH=7，5%盐度，以酵母粉作为碳源。偶氮还原酶、NADH-DCIP酶是主要的降解酶，盐度抑制了这两种酶的活性。酸性金黄G的偶氮键对称断裂成4-氨基苯磺酸和对氨基二苯胺，进一步降解为二苯胺、苯胺、2-庚酮肟等，降解后产物毒性降低。菌株对不同浓度的酸性金黄G具有耐受性，具有良好的应用潜力。该研究以期为嗜盐菌处理高盐印染废水提供菌种资源和理论依据。

关键词: 偶氮染料, 分离, 脱色, 酸性金黄G, 嗜盐菌

Abstract:

High salinity in textile wastewater limited the application of biological method in textile wastewater. Isolation of halophilic microorganisms is important for improving the treatment efficiency of high salinity textile wastewater. A strain was isolated from active sludge of textile wastewater, which can decolorize metanil yellow. The bacteria were identified by 16S rDNA. The degradation mechanism was analyzed. The results showed the bacteria had the highest homology with Exiguobaterium strain ACCC11618 and belong to the genus. At 5% salinity, more than 95% metanil yellow was decolorized by S2 within 8h. The optimum decolorization condition was 5% salinity, pH 7, at 30℃, and yeast powder as carbon source. Azo reductase and NADH-DCIP are the main degrading enzymes. Salinity inhibits the activity of these two enzymes. The azo bond of metanil yellow was symmetrically broken into 4-aminobenzene sulfonic acid and p-aminobenzidine, which further degraded into diphenylamine, aniline, and 2-heptanone oxime. The toxicity was decreased after decolorization. The strain could decolorize metanil yellow at different concentrations, which showed good application potential. This study is expected to provide species resources and theoretical basis for treatment high salinity textile wastewater.

Key words: azo dye, separation, decolorization characteristics, metanil yellow G, halophilic

中图分类号:

X170

田芳, 郭光, 丁克强, 杨凤, 刘翀, 王慧雅. 降解偶氮染料嗜盐菌的分离、降解特性及机制[J]. 化工进展, 2023, 42(4): 2115-2121.

TIAN Fang, GUO Guang, DING Keqiang, YANG Feng, LIU Chong, WANG Huiya. Isolation of halophilic bacterium and their decolorization characteristics and mechanism of azo dyes[J]. Chemical Industry and Engineering Progress, 2023, 42(4): 2115-2121.

图/表 12

参考文献 26

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酶的种类	不同盐度下的酶活性
酶的种类	1%	3%	5%	7%	9%
漆酶/µmol·mg^-1·min^-1	0.031	0.75	0.71	0.21	0.10
锰过氧化物酶/µmol·mg^-1·min^-1	0.078	0.083	0.084	0.082	0.078
偶氮还原酶/µmol·mg^-1·min^-1	0.29	1.05	1.82	1.23	0.136
NADH-DCIP/µg^-1·mg ^-1 ·min^-1	5.22	10.45	16.08	4.34	1.182

酶的种类	不同盐度下的酶活性
酶的种类	1%	3%	5%	7%	9%
漆酶/µmol·mg^-1·min^-1	0.031	0.75	0.71	0.21	0.10
锰过氧化物酶/µmol·mg^-1·min^-1	0.078	0.083	0.084	0.082	0.078
偶氮还原酶/µmol·mg^-1·min^-1	0.29	1.05	1.82	1.23	0.136
NADH-DCIP/µg^-1·mg ^-1 ·min^-1	5.22	10.45	16.08	4.34	1.182

项目	黄瓜			水稻
项目	发芽率/%	胚芽/cm	胚根/cm	发芽率/%	胚芽/cm	胚根/cm
水	100	5.7±0.8	4.9±0.6	100	4.8±0.9	3.5±0.5
酸性金黄G	46.6	3.9±0.6	3.2±0.7	66.6	3.1±0.6	2.4±0.5
降解产物	100	5.0±0.7	4.5±0.9	100	4.7±0.7	3.3±0.6

项目	黄瓜			水稻
项目	发芽率/%	胚芽/cm	胚根/cm	发芽率/%	胚芽/cm	胚根/cm
水	100	5.7±0.8	4.9±0.6	100	4.8±0.9	3.5±0.5
酸性金黄G	46.6	3.9±0.6	3.2±0.7	66.6	3.1±0.6	2.4±0.5
降解产物	100	5.0±0.7	4.5±0.9	100	4.7±0.7	3.3±0.6

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