链格孢菌对库存喷气燃料性质的影响

doi:10.16085/j.issn.1000-6613.2019-0555

摘要/Abstract

摘要：

研究链格孢菌在生长过程中对库存喷气燃料总酸值等性质的影响。以菌丝干重为指标，通过三因素三水平正交试验，筛选了链格孢菌最佳培养条件；构建喷气燃料、培养基、菌液培养体系，研究了链格孢菌生长繁殖对喷气燃料总酸值以及银片腐蚀试验、水反应试验、表面张力等的影响；构建以喷气燃料替代物十二烷为唯一碳源的培养体系，通过气相色谱检测十二烷残留率，研究了链格孢菌生长繁殖与十二烷的降解特征。链格孢菌最佳培养条件为30℃、初始pH为8、钠元素浓度为0；链格孢菌在生长繁殖过程中，能产生酸性物质，增加喷气燃料总酸值、降低水相pH，能产生表面活性物质，降低喷气燃料表面张力、水相表面张力，但培养周期内对银片腐蚀试验、水反应试验无显著影响；链格孢菌能以十二烷为唯一碳源进行生长繁殖。链格孢菌在生长繁殖过程中，能增加喷气燃料总酸值，降低喷气燃料、水相表面张力以及水相pH。

关键词: 特征真菌, 链格孢菌, 喷气燃料, 性质, 降解

Abstract:

The objective of this study was to research the effect of Alternaria alternata on the total acid value and other properties of stock jet fuel during growth. With the dry weight of mycelium as the index, the optimal culture conditions of Alternaria alternata were selected by three-factor and three-level orthogonal experiments. The jet fuel, medium and fungus culture system were constructed to study the effects of Alternaria alternata growth and proliferation on the total acid value of jet fuel, the silver sheet corrosion test, water reaction test, and surface tension, etc. The culture system with dodecane as the sole carbon source was constructed and the residual rate of dodecane was detected by gas chromatography to study the growth and reproduction of Alternaria alternata and the degradation characteristics of dodecane. The optimal culture conditions of Alternaria alternata were 30℃, the initial pH was 8, and the sodium concentration was 0mg/L. With acidic substances and surface-active substances produced, the total acid value of jet fuel was increased, and the aqueous pH, the surface tension of jet fuel and water were reduced during Alternaria alternata growth and reproduction. Alternaria alternata could grow and multiply with dodecane as the sole carbon source. It was concluded that in the process of growth and reproduction, Alternaria alternata could increase the total acid value of jet fuel, reduce the surface tension of jet fuel, water phase and the aqueous pH.

Key words: characteristic fungi, Alternaria alternata, jet fuel, property, degradation

中图分类号:

TE 626.23

孙新枫,熊云,牛明明,许世海,苏鹏,范林君,毛澍坤. 链格孢菌对库存喷气燃料性质的影响[J]. 化工进展, 2019, 38(10): 4504-4510.

Xinfeng SUN,Yun XIONG,Mingming NIU,Shihai XU,Peng SU,Linjun FAN,Shukun MAO. Effect of Alternaria alternata on the properties of stock jet fuel[J]. Chemical Industry and Engineering Progress, 2019, 38(10): 4504-4510.

图/表 12

表1 正交试验因素及水平设计表

水平	温度（A）/℃	pH（B）	钠元素浓度（C）/mg·L^-1
1	25	6	0
2	30	7	100
3	35	8	200

图1 十二烷标准曲线

表2 正交试验结果及极差分析

组别	因素			菌丝干重/g
组别	A	B	C	菌丝干重/g
1	1	1	1	0.0203
2	1	2	2	0.0183
3	1	3	3	0.0274
4	2	1	2	0.0146
5	2	2	3	0.0139
6	2	3	1	0.0404
7	3	1	3	0.0131
8	3	2	1	0.0131
9	3	3	2	0.0266
T ₁	0.066	0.048	0.0738
T ₂	0.0689	0.0453	0.0595
T ₃	0.0528	0.0944	0.0544
$T ˉ$ $1$	0.022	0.016	0.0246	A ₂ B ₃ C ₁
$T ˉ$ $2$	0.022966667	0.0151	0.019833333
$T ˉ$ $3$	0.0176	0.031466667	0.018133333
R	0.005366667	0.016366667	0.006466667	B >C> A

表2 正交试验结果及极差分析

组别	因素			菌丝干重/g
组别	A	B	C	菌丝干重/g
1	1	1	1	0.0203
2	1	2	2	0.0183
3	1	3	3	0.0274
4	2	1	2	0.0146
5	2	2	3	0.0139
6	2	3	1	0.0404
7	3	1	3	0.0131
8	3	2	1	0.0131
9	3	3	2	0.0266
T ₁	0.066	0.048	0.0738
T ₂	0.0689	0.0453	0.0595
T ₃	0.0528	0.0944	0.0544
$T ˉ$ $1$	0.022	0.016	0.0246	A ₂ B ₃ C ₁
$T ˉ$ $2$	0.022966667	0.0151	0.019833333
$T ˉ$ $3$	0.0176	0.031466667	0.018133333
R	0.005366667	0.016366667	0.006466667	B >C> A

图2 因素水平趋势图

表3 方差分析表

因素	平方和SS	自由度DF	均方MS	F值
A	0.0000491378	2	0.0000245689	1.045
B	0.000508108	2	0.000254054	10.802
C	0.00006818	2	0.00003409	1.450
误差	0.0000470366	2	0.0000235183
总和	0.000672462	8

图3 验证实验图

图4 喷气燃料总酸值变化趋势

图5 水相表面张力变化

图6 喷气燃料表面张力变化

图7 水相pH变化趋势

图8 十二烷残留率曲线

图9 链格孢菌生长曲线

参考文献 19

1	李鹏 . 喷气燃料杀菌剂的应用基础研究[D]. 重庆：陆军勤务学院, 2017.
	LI Peng . Basic research on applications of different bactericides in jet fuel[D]. Chongqing： Army Logistics University of PLA, 2017.
2	许世海, 熊云, 刘晓 . 液体燃料的性质及应用[M]. 北京: 中国石化出版社, 2010.
	XU Shihai , XIONG Yun , LIU Xiao . The properties and application of liquid fuels[M]. Beijing: China Petrochemical Press, 2010.
3	LANGTON R , CLARK C , HEWIT M , et al . Aircraft fuel systems[M]. New Jersey: John Wiley & Sons, Ltd., 2009.
4	PASSMAN F J .Microbial contamination and its control in fuels and fuel systems since 1980: a review[J]. International Biodeterioration and Biodegradation, 2013(81): 88-104.
5	崔艳雨, 陈世一, 杜金杰 . 微生物对航空煤油影响的实验研究[J]. 中国民航大学学报, 2010(6): 22-25.
	CUI Yanyu , CHEN Shiyi , DU Jinjie . Experimental studies of aviation fuel’s indexes influenced by microorganism[J]. Journal of Civil Aviation University of China, 2010(6): 22-25.
6	牛明明, 熊云, 孙新枫 . 喷气燃料中微生物污染及检测方法概述[J]. 当代化工, 2019, 48(2): 410-414.
	NIU Mingming , XIONG Yun , SUN Xinfeng . Overview of microbial contamination and detection methods in jet fuel[J]. Contemporary Chemical Industry, 2019, 48(2): 410-414.
7	龙泉芝 . 特征真菌对喷气燃料的影响及对策研究[D]. 重庆：后勤工程学院, 2015.
	LONG Quanzhi . The influence of typical fungi on jet fuel and countermeasures research[D]. Chongqing: Logistical Engineering University, 2015.
8	龙泉芝, 熊云, 倪康康, 等 . Amorphotheca resinae对喷气燃料影响试验研究[J]. 后勤工程学院学报, 2015(5): 56-59.
	LONG Quanzhi , XIONG Yun , NI Kangkang , et al . Experimental study on effects of Amorphotheca resinae on jet fuel[J]. Journal of Logistical Engineering University, 2015(5): 56-59.
9	IATA . Guidance material on microbiological contamination in aircraft fuel tanks(5th) [S]. Canada: IATA, 2015.
10	FAZIO P C , Materials American Society for testing and standard guide for microbial contamination in fuels and fuel systems [S] . American: ASTM, 2012.
11	钱存柔, 黄仪秀 . 微生物学实验教程[M]. 2版. 北京: 北京大学出版社, 2008.1.
	QIAN Cunrou , HUANG Yixiu . Laboratory experiments in microbiology[M]. 2nd ed. Beijing: Peking University Press, 2008.1.
12	徐锐光, 赵恒, 郭钰坚 . 电子天平的不确定度对恒重试验的影响[J]. 中国医疗器械信息, 2010(4): 71-73.
	XU Ruiguang , ZHAO Heng , GUO Yujian . Effect of uncertainty of electronic balance on the experimentation of constant weight[J]. China Medical Device Information, 2010(4): 71-73.
13	国家技术监督局 . 喷气燃料总酸值测定法: GB/T 12574—1990[S]. 北京: 中国标准出版社, 1990
	General Administration of Technical Supervision of the People’s Republic of China . Jet fuels- Determination of total acid number: GB/T 12574—1990[S]. Beijing: Standards Press of China, 1990.
14	国家质量监督检验检疫总局 . 表面活性剂表面张力的测定: GB/T 22237—2008[S]. 北京: 中国标准出版社, 2008.
	General Administration of Quality Supervision, Inspection and Quarantine of the People’s Republic of China . Surface active agents determination of surface tension: GB/T 22237—2008[S]. Beijing: Standards Press of China, 2008.
15	国家质量监督检验检疫总局 . 航空燃料水反应试验法: GB/T 1793—2008[S]. 北京: 中国标准出版社, 2008.
	General Administration of Quality Supervision, Inspection and Quarantine of the People’s Republic of China . Standard test method for water reaction of aviation fuels: GB/T 1793—2008[S]. Beijing: Standards Press of China, 2008.
16	中国石油化工总公司 . 喷气燃料银片腐蚀试验法: SH/T 0023—1990[S]. 北京: 中国石化出版社, 1992.
	Sinopec Group of China . Test method for corrosiveness to silver strip: SH/T 0023—1990[S]. Beijing: China Petrochemical Press, 1992.
17	裴鑫岩 . 航空煤油超临界换热与氧化结焦理论与实验研究[D]. 北京: 清华大学, 2016.
	PEI Xinyan . Numerical and experimental study of heat transfer characteristics and oxidation deposition of aviation kerosene at supercritical pressure[D]. Beijing: Tsinghua University, 2016.
18	于维铭 . 航空煤油替代燃料火焰传播速度与反应动力学机理研究[D]. 北京: 清华大学, 2014.
	YU Weiming . Study on flame speed and chemical reaction mechanism for alternative fuels of aviation kerosene[D]. Beijing: Tsinghua University, 2014.
19	朱明华, 胡坪 . 仪器分析[M]. 北京: 高等教育出版社, 2011.
	ZHU Minghua , HU Ping . Instrumental analysis[M]. Beijing: Higher Education Press, 2011.

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