亚麻刺盘孢表面磁纳米修饰及其羟化去氢表雄酮

doi:10.16085/j.issn.1000-6613.2017-0279

化工进展 ›› 2017, Vol. 36 ›› Issue (11): 4194-4199.DOI: 10.16085/j.issn.1000-6613.2017-0279

亚麻刺盘孢表面磁纳米修饰及其羟化去氢表雄酮

李刚¹, 李恒¹, 陆震鸣¹, 李会¹, 龚劲松¹, 许正宏², 史劲松¹

1. 江南大学药学院, 江苏无锡 214122;
2. 江南大学生物工程学院, 江苏无锡 214122

收稿日期:2017-02-22 修回日期:2017-03-18 出版日期:2017-11-05 发布日期:2017-11-05
通讯作者: 史劲松,教授,博士,主要从事生物制药研究。
作者简介:李刚(1992-),男,硕士研究生。E-mail:leegang1992@163.com。
基金资助:
国家高技术研究发展计划重大项目（2011AA02A211）及国家自然科学基金项目（21206055、21406088）。

Magnetic nanoparticles modification of Colletotrichum lini and its application in bio-hydroxylation of dehydroepiandrosterone

LI Gang¹, LI Heng¹, LU Zhenming¹, LI Hui¹, GONG Jinsong¹, XU Zhenghong², SHI Jinsong¹

1. School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, Jiangsu, China;
2. School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China

Received:2017-02-22 Revised:2017-03-18 Online:2017-11-05 Published:2017-11-05

摘要/Abstract

摘要： 以阳离子聚电解质聚烯丙基胺盐酸盐（PAAH）对共沉淀法制备的Fe₃O₄磁性纳米颗粒进行包裹，再以此对亚麻刺盘孢Colletotrichum lini ST-1进行表面修饰，制备具有磁性的菌体复合物。通过SEM、XRD、VSM和zeta电位分析表明，PAAH修饰的Fe₃O₄能够稳定均一地结合在C.lini ST-1菌体表面，形成的磁性复合物具有超顺磁性，可利用外加磁场对菌体进行回收。本研究利用该磁性复合物对去氢表雄酮（DHEA）进行生物羟化，制备三羟基雄甾烯酮（7α，15α-diOH-DHEA），通过回收菌体完成了4个批次的转化，产物7α，15α-diOH-DHEA的浓度为10.83g/L，约为游离菌体转化效果的3倍。

关键词: 磁纳米颗粒, 阳离子聚电解质, 三羟基雄甾烯酮, 生物转化

Abstract: The cationic polyelectrolyte modified magnetic nanoparticles were prepared through surface modification of Fe₃O₄ nanoparticles that were prepared by co-precipitation method with poly(allylamine hydrochloride)(PAAH). The fungi of Colletotrichum lini ST-1 was then coated with the modified Fe₃O₄ nanoparticles to form magnetic composites. The magnetic composites were characterized by SEM,XRD,VSM,and zeta potential. The results demonstrated that the modified Fe₃O₄ nanoparticles adsorbed onto C. lini ST-1 cell surface stably and uniformly by electrostatic adsorption. The magnetic composites exhibited superparamagnetic properties, which could be utilized for strain recovery. During the biotransformation of dehydroepiandrosterone (DHEA)to 3β, 7α, 15α-trihydroxy-5-androsten-17-one(7α,15α-diOH-DHEA),the magnetic composites could be recovered by the application of external magnetic field for 4 batches. The concentration of 7α, 15α-diOH-DHEA was 10.83g/L, which was about 3 times higher than that of the free cells.

Key words: magnetic nanoparticle, cationic polyelectrolyte, 3β,7α,15α-trihydroxy-5-androsten-17-one, biotransformation

中图分类号:

Q814.9

李刚, 李恒, 陆震鸣, 李会, 龚劲松, 许正宏, 史劲松. 亚麻刺盘孢表面磁纳米修饰及其羟化去氢表雄酮[J]. 化工进展, 2017, 36(11): 4194-4199.

LI Gang, LI Heng, LU Zhenming, LI Hui, GONG Jinsong, XU Zhenghong, SHI Jinsong. Magnetic nanoparticles modification of Colletotrichum lini and its application in bio-hydroxylation of dehydroepiandrosterone[J]. Chemical Industry and Engineering Progress, 2017, 36(11): 4194-4199.

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亚麻刺盘孢表面磁纳米修饰及其羟化去氢表雄酮

Magnetic nanoparticles modification of Colletotrichum lini and its application in bio-hydroxylation of dehydroepiandrosterone

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