ClostridiumsaccharobutylicumDSM13864细胞表面理化特性及固定化细胞产丁醇的性能

doi:10.16085/j.issn.1000-6613.2015.12.014

化工进展 ›› 2015, Vol. 34 ›› Issue (12): 4214-4219.DOI: 10.16085/j.issn.1000-6613.2015.12.014

ClostridiumsaccharobutylicumDSM13864细胞表面理化特性及固定化细胞产丁醇的性能

陈强, 董晋军, 许国超, 韩瑞枝, 倪晔

江南大学工业生物技术教育部重点实验室, 江南大学生物工程学院, 江苏无锡 214122

收稿日期:2015-03-18 修回日期:2015-04-16 出版日期:2015-12-05 发布日期:2015-12-05
通讯作者: 倪晔,教授,主要研究方向为生物催化和酶工程。E-mailyni@jiangnan.edu.cn。
作者简介:陈强(1989—),男,硕士研究生。
基金资助:
国家973计划(2011CB710800)及国家自然科学基金(21276112,31401634)项目。

Enhanced butanol production by Clostridium saccharobutylicum DSM 13864: Physicochemical property and immobilization

CHEN Qiang, DONG Jinjun, XU Guochao, HAN Ruizhi, NI Ye

The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China

Received:2015-03-18 Revised:2015-04-16 Online:2015-12-05 Published:2015-12-05

摘要/Abstract

摘要： 糖丁基梭菌Clostridium saccharobutylicum DSM 13864能利用多种糖类为底物发酵产丁醇。本文研究了该菌体细胞表面的理化特性,并以砖块作为细胞固定化材料进行丁醇发酵。采用细菌吸附有机溶剂(MATS)法证明糖丁基梭菌细胞表面有强烈的亲水性,并且等电点在pH值为3左右,这些特性有利于菌体与表面亲水多孔的砖块吸附。在60g/L葡萄糖发酵培养基中,以5～8目砖块作为固定化材料,流速为1.1L/min,发酵48h后,丁醇的浓度、得率和生产率分别达到11.02g/L、0.18g/g和0.23g/(L·h),相比悬浮细胞发酵分别提高了10.53%、5.88%和9.52%。结果表明:砖块作为一种固定化材料可有效提高糖丁基梭菌的发酵产丁醇水平。

关键词: 糖丁基梭菌, 丁醇, 固定化, 细菌吸附有机溶剂, 砖块

Abstract: Clostridium saccharobutylicum DSM 13864 is a solventogenic strain capable of producing ABE solvents (acetone,butanol and ethanol) from various saccharides. To validate its potential in ABE fermentation with cell immobilization,the physicochemical properties of C. saccharobutylicum DSM 13864 cells were investigated. The microbial adhesion to solvents (MATS) analysis shows that the cell surface is hydrophilic due to its strong electron-donating property,and negatively charged at pH above 3. Brick was used as an immobilization material in ABE fermentation with C. saccharobutylicum. At a flow rate of 1.1L/min through the immobilized C. saccharobutylicum,the titer and yield of butanol reached 11.02g/L and 0.18g/g respectively,and butanol productivity was 0.23g/(L·h) in batch fermentation,representing improvements of 10.53%,5.88% and 9.52% compared with free cell fermentation,respectively. The results suggest that cell immobilization with brick is efficacious in enhancing butanol production by C. saccharobutylicum.

Key words: Clostridium saccharobutylicum, butanol, immobilization, microbial adhesion to solvents (MATS), brick

中图分类号:

Q815

陈强, 董晋军, 许国超, 韩瑞枝, 倪晔. ClostridiumsaccharobutylicumDSM13864细胞表面理化特性及固定化细胞产丁醇的性能[J]. 化工进展, 2015, 34(12): 4214-4219.

CHEN Qiang, DONG Jinjun, XU Guochao, HAN Ruizhi, NI Ye. Enhanced butanol production by Clostridium saccharobutylicum DSM 13864: Physicochemical property and immobilization[J]. Chemical Industry and Engineering Progree, 2015, 34(12): 4214-4219.

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ClostridiumsaccharobutylicumDSM13864细胞表面理化特性及固定化细胞产丁醇的性能

Enhanced butanol production by Clostridium saccharobutylicum DSM 13864: Physicochemical property and immobilization

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